Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,068,285 Δ1 bp intergenic (+31/+226) ymdF → / ← rutG KGG family protein/pyrimidine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,068,2850T.100.0% 306.4 / NA 73intergenic (+31/+226)ymdF/rutGKGG family protein/pyrimidine permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (36/37);  total (36/37)

CGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACT  >  NC_000913/1068167‑1068412
                                                                                                                      |                                                                                                                               
cGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCaa                                                                                                                   <  2:1609916/131‑1 (MQ=255)
cGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCaa                                                                                                                   >  1:1609916/1‑131 (MQ=255)
 gggggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATc                                                                                                          >  1:1346266/1‑139 (MQ=255)
     gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCAccg                                                                                                      >  1:1176281/1‑139 (MQ=255)
     gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCAccg                                                                                                      >  1:1313529/1‑139 (MQ=255)
           tCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGAGCTCGTCAAAAAGCCATCACCGCCGGTTa                                                                                               <  1:1109181/139‑1 (MQ=255)
               gAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                                       <  2:1193271/128‑1 (MQ=255)
               gAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                                       >  1:1193271/1‑128 (MQ=255)
                aaTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                           <  1:1728617/139‑1 (MQ=255)
                aaTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                           <  1:138577/139‑1 (MQ=255)
                   gACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtg                                                                                        >  1:1009660/1‑139 (MQ=255)
                    aCCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtgg                                                                                       >  1:1777204/1‑139 (MQ=255)
                            cgcgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACt                                                                               >  2:517143/1‑139 (MQ=255)
                            cgcgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACt                                                                               <  2:1176281/139‑1 (MQ=255)
                            cgcgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACt                                                                               <  2:1313529/139‑1 (MQ=255)
                                  tCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGAc                                                                         <  1:517143/139‑1 (MQ=255)
                                        gCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGt                                                                   <  1:269997/139‑1 (MQ=255)
                                           ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAg                                                                >  2:702036/1‑139 (MQ=255)
                                            gTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                           <  1:410922/111‑1 (MQ=255)
                                            gTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                           >  2:410922/1‑111 (MQ=255)
                                             tAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAcc                                                                                             <  2:1440714/108‑1 (MQ=255)
                                             tAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAcc                                                                                             >  1:1440714/1‑108 (MQ=255)
                                             tAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCt                                                              >  2:362243/1‑139 (MQ=255)
                                                aaaaGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACt                                                                               <  2:1109262/119‑1 (MQ=255)
                                                aaaaGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACt                                                                               >  1:1109262/1‑119 (MQ=255)
                                                    ggtggtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAcc                                                                                             <  2:1590276/101‑1 (MQ=255)
                                                    ggtggtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCCGTTAcc                                                                                             >  1:1590276/1‑101 (MQ=255)
                                                       ggtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                           <  2:495058/100‑1 (MQ=255)
                                                       ggtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                           >  1:495058/1‑100 (MQ=255)
                                                       ggtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgc                                                    >  1:1210876/1‑139 (MQ=255)
                                                        gtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                           >  1:1795143/1‑99 (MQ=255)
                                                        gtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                           <  2:1795143/99‑1 (MQ=255)
                                                          aaGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATGAATGACGAATCATTTTTGCTGTAg                                                                                                                           <  1:1242548/65‑1 (MQ=255)
                                                          aaGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAg                                                                                                                           >  2:1242548/1‑65 (MQ=255)
                                                             agCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATg                                              <  2:1777204/139‑1 (MQ=255)
                                                                aGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATg                                              <  1:148684/136‑1 (MQ=255)
                                                                aGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATg                                              >  2:148684/1‑136 (MQ=255)
                                                                  tCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTAt                                         <  1:362243/139‑1 (MQ=255)
                                                                  tCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTAt                                         <  2:1210876/139‑1 (MQ=255)
                                                                    aCGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCa                                       >  1:1620504/1‑139 (MQ=255)
                                                                     cGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCaa                                                                                                                   >  2:467924/1‑62 (MQ=255)
                                                                     cGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCaa                                                                                                                   <  1:467924/62‑1 (MQ=255)
                                                                      ggCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAgg                                     <  1:764070/139‑1 (MQ=255)
                                                                           aaGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                           >  2:1121130/1‑80 (MQ=255)
                                                                           aaGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                           <  1:1121130/80‑1 (MQ=255)
                                                                           aaGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTAc                                >  2:1391917/1‑139 (MQ=255)
                                                                            aGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTg                                                        <  1:1663555/114‑1 (MQ=255)
                                                                            aGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTg                                                        >  2:1663555/1‑114 (MQ=255)
                                                                             gCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCa                              <  2:1009660/139‑1 (MQ=255)
                                                                                       accGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCAt                                                                                                           <  1:819535/51‑1 (MQ=255)
                                                                                       accGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCAt                                                                                                           >  2:819535/2‑52 (MQ=255)
                                                                                            gCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCaaa                                                                                                                  <  2:262008/40‑1 (MQ=255)
                                                                                            gCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCaaa                                                                                                                  >  1:262008/1‑40 (MQ=255)
                                                                                            gCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtggt                                                                                      >  1:896256/1‑68 (MQ=255)
                                                                                            gCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtggt                                                                                      <  2:896256/68‑1 (MQ=255)
                                                                                             cTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGATTGCAatatat              >  2:1003050/1‑139 (MQ=255)
                                                                                                aTCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGcc                                                                                                             >  2:1618191/1‑41 (MQ=255)
                                                                                                aTCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGcc                                                                                                             <  1:1618191/41‑1 (MQ=255)
                                                                                                     tGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTg      >  1:924717/1‑139 (MQ=255)
                                                                                                       aCGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGGAGATTGCAATATATTGAATTTGCa    <  1:1243359/139‑1 (MQ=255)
                                                                                                         cgaatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaaga                         <  1:1306975/112‑1 (MQ=255)
                                                                                                         cgaatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaaga                         >  2:1306975/6‑117 (MQ=255)
                                                                                                          gaatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACt  <  2:376890/135‑1 (MQ=255)
                                                                                                           aatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                           >  2:826770/4‑49 (MQ=255)
                                                                                                           aatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                           <  2:208618/46‑1 (MQ=255)
                                                                                                           aatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                           <  1:826770/46‑1 (MQ=255)
                                                                                                           aatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                                           >  1:208618/4‑49 (MQ=255)
                                                                                                           aatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAgg                                     <  2:40635/100‑1 (MQ=255)
                                                                                                           aatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAgg                                     >  1:40635/4‑103 (MQ=255)
                                                                                                           aatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGATTGCa                    >  2:855329/4‑120 (MQ=255)
                                                                                                           aatcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGATTGCa                    <  1:855329/117‑1 (MQ=255)
                                                                                                             tcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaaga                         <  2:1069469/112‑1 (MQ=255)
                                                                                                             tcATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATCTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaaga                         >  1:1069469/2‑113 (MQ=255)
                                                                                                                      |                                                                                                                               
CGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACT  >  NC_000913/1068167‑1068412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: