Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarate hydratase (fumarase C),aerobic Class II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,685,5370C.100.0% 305.2 / NA 73coding (1052/1404 nt)fumCfumarate hydratase (fumarase C),aerobic Class II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (39/34);  total (39/34)

TTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCAC  >  NC_000913/1685408‑1685669
                                                                                                                                 |                                                                                                                                    
ttCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGtt                                                                                                                            >  2:957654/1‑139 (MQ=255)
   ggTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGAt                                                                                                                         >  2:504201/1‑139 (MQ=255)
        aaTACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCa                                                                                                                    <  2:811200/139‑1 (MQ=255)
          tACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCAcg                                                                                                                  >  1:973762/1‑139 (MQ=255)
              cACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCAcgtcgt                                                                                                              >  1:157850/1‑139 (MQ=255)
               aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGtt                                                                                                             >  2:1794146/1‑139 (MQ=255)
               aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGtt                                                                                                             >  2:1268025/1‑139 (MQ=255)
                 tGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGccccc                                                                                                                                  >  1:1087268/1‑117 (MQ=255)
                 tGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGccccc                                                                                                                                  <  2:1087268/117‑1 (MQ=255)
                  gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCa                                                                                                                    <  1:678528/129‑1 (MQ=255)
                  gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCa                                                                                                                    <  1:717717/129‑1 (MQ=255)
                  gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCa                                                                                                                    >  2:678528/1‑129 (MQ=255)
                  gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCa                                                                                                                    >  2:717717/1‑129 (MQ=255)
                  gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTccc                                                                                                          <  1:504201/139‑1 (MQ=255)
                   cgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTcccc                                                                                                         >  2:451674/1‑139 (MQ=255)
                   cgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTcccc                                                                                                         >  1:1758552/1‑139 (MQ=255)
                    gcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCa                                                                                                        >  2:342090/1‑139 (MQ=255)
                           ttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTg                                                                                                 >  1:82637/1‑139 (MQ=255)
                           ttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTg                                                                                                 <  1:451674/139‑1 (MQ=255)
                               tAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                             <  2:1628962/139‑1 (MQ=255)
                               tAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                             <  2:585393/139‑1 (MQ=255)
                                  aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                          >  1:499809/1‑139 (MQ=255)
                                  aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                          >  1:296579/1‑139 (MQ=255)
                                  aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                          >  1:619227/1‑139 (MQ=255)
                                    cTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCagag                                                                                        <  2:157850/139‑1 (MQ=255)
                                          aTGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGt                                                                                  <  2:1758552/139‑1 (MQ=255)
                                            gCCATCTGCCAGCAAGCTCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                >  2:1146229/1‑139 (MQ=255)
                                            gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                <  1:1794146/139‑1 (MQ=255)
                                                 cTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGcc                                                                           >  2:50967/1‑139 (MQ=255)
                                                  tGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCt                                                                          <  1:50967/139‑1 (MQ=255)
                                                        cAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCa                                                                                                                    >  1:1681483/1‑91 (MQ=255)
                                                        cAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCa                                                                                                                    <  2:1681483/91‑1 (MQ=255)
                                                        cAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACt                                                                    <  2:409571/139‑1 (MQ=255)
                                                          aGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACtgt                                                                  >  2:223320/1‑139 (MQ=255)
                                                            cgcACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTgtgt                                                                >  2:611017/1‑139 (MQ=255)
                                                              cACCGATTGCAGGAAGTTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCa                                                                                                                    >  2:867355/1‑85 (MQ=255)
                                                              cACCGATTGCAGGAAGTTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCa                                                                                                                    <  1:867355/85‑1 (MQ=255)
                                                              cACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTg                                                              >  2:1177836/1‑139 (MQ=255)
                                                                           aaaTTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCACCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTccc                                                 <  2:1210221/139‑1 (MQ=255)
                                                                              ttGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCgt                                              <  1:1146229/139‑2 (MQ=255)
                                                                              ttGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCgg                                              <  1:1268025/139‑1 (MQ=255)
                                                                                     tCACCATTTGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGAtt                                       >  1:272759/1‑139 (MQ=255)
                                                                                      cACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCa                                                                                      >  1:1108597/1‑91 (MQ=255)
                                                                                      cACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCa                                                                                      <  2:1108597/91‑1 (MQ=255)
                                                                                      cACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGcc                                                                           >  2:737949/1‑102 (MQ=255)
                                                                                      cACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGcc                                                                           >  1:1654792/1‑102 (MQ=255)
                                                                                      cACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGcc                                                                           <  2:1654792/102‑1 (MQ=255)
                                                                                      cACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGcc                                                                           <  1:737949/102‑1 (MQ=255)
                                                                                      cACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTg                                      >  2:1155118/1‑139 (MQ=255)
                                                                                      cACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTg                                      <  2:296579/139‑1 (MQ=255)
                                                                                            tGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGc                                <  1:957654/139‑1 (MQ=255)
                                                                                              gACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                      >  1:1380229/1‑99 (MQ=255)
                                                                                              gACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                      <  2:1380229/99‑1 (MQ=255)
                                                                                              gACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTc                                                   >  1:1690045/1‑118 (MQ=255)
                                                                                              gACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTc                                                   <  2:1690045/118‑1 (MQ=255)
                                                                                              gACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGccc                              <  2:82637/139‑1 (MQ=255)
                                                                                              gACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGccc                              >  1:540081/1‑139 (MQ=255)
                                                                                              gACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGccc                              <  2:499809/139‑1 (MQ=255)
                                                                                                   aaGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTg                                                                                                                           <  2:1510635/41‑1 (MQ=255)
                                                                                                   aaGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTg                                                                                                                           >  1:1510635/1‑41 (MQ=255)
                                                                                                   aaGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                          <  2:46637/74‑1 (MQ=255)
                                                                                                   aaGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                          >  1:46637/1‑74 (MQ=255)
                                                                                                   aaGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTg                                                              <  2:253064/102‑1 (MQ=255)
                                                                                                   aaGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTg                                                              >  1:253064/1‑102 (MQ=255)
                                                                                                         ttcagttcaAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAAAGCAGAGCAGGGTTAATGCATCACACGGTGTTGGGTTCACTTTCCTCGGCATGATTGAGCTGCCCGGCTCATTTTc                   >  1:438264/1‑139 (MQ=255)
                                                                                                             gttcaAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                      <  1:956171/84‑1 (MQ=255)
                                                                                                             gttcaAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCggg               >  2:596287/1‑139 (MQ=255)
                                                                                                             gttcaAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGAAGAGCATGGTTAATGCCTcaca                                                                      >  2:956171/1‑84 (MQ=255)
                                                                                                              ttcaAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCt                                                                                                  >  1:258126/1‑55 (MQ=255)
                                                                                                              ttcaAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCt                                                                                                  <  2:258126/55‑1 (MQ=255)
                                                                                                                        ccGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                      <  2:583310/73‑1 (MQ=255)
                                                                                                                        ccGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                      >  1:583310/1‑73 (MQ=255)
                                                                                                                        ccGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcac                                                                       >  2:552890/1‑72 (MQ=255)
                                                                                                                        ccGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcac                                                                       <  1:552890/72‑1 (MQ=255)
                                                                                                                          ggAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCAc  <  1:1177836/139‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                    
TTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCAC  >  NC_000913/1685408‑1685669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: