Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,821,867 (A)8→7 intergenic (‑248/+51) chbB ← / ← osmE N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS/osmotically‑inducible lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,821,8600A.100.0% 220.1 / NA 53intergenic (‑241/+58)chbB/osmEN,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS/osmotically‑inducible lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (29/24);  total (29/24)

GGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTA  >  NC_000913/1821740‑1821990
                                                                                                                        |                                                                                                                                  
ggTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATgg                                                                                                                 <  1:981979/139‑1 (MQ=255)
            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                     <  1:1339084/139‑1 (MQ=255)
            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                     >  2:1339084/1‑139 (MQ=255)
            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCATCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                     >  2:938295/1‑139 (MQ=255)
                      tttCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAg                                                                                           >  1:722267/1‑139 (MQ=255)
                         cTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCtt                                                                                        >  2:1168175/1‑139 (MQ=255)
                              aCCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAGACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGa                                                                                   >  1:1190697/1‑139 (MQ=255)
                              aCCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGCGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGa                                                                                   >  2:291244/1‑139 (MQ=255)
                                   ataAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCt                                                                                         >  2:1529809/1‑128 (MQ=255)
                                   ataAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCt                                                                                         <  1:1529809/128‑1 (MQ=255)
                                             tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGcc                                                                                                               >  1:180095/1‑96 (MQ=255)
                                             tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGcc                                                                                                               <  2:180095/96‑1 (MQ=255)
                                              ctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                   <  1:1804544/139‑1 (MQ=255)
                                                ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGTCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                 <  1:1787645/139‑1 (MQ=255)
                                                  ttGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGc                                                               <  1:1168175/139‑1 (MQ=255)
                                                     aCCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCtg                                                            >  2:350047/1‑139 (MQ=255)
                                                         gtgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGAt                                                                          <  2:190223/121‑1 (MQ=255)
                                                         gtgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGAt                                                                          >  1:190223/1‑121 (MQ=255)
                                                          tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                       >  1:554754/1‑139 (MQ=255)
                                                               aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCa                                                                                             <  1:606362/96‑1 (MQ=255)
                                                               aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCa                                                                                             >  2:606362/1‑96 (MQ=255)
                                                               aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                  >  2:57350/1‑139 (MQ=255)
                                                               aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                  <  1:640781/139‑1 (MQ=255)
                                                               aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                  >  2:1358511/1‑139 (MQ=255)
                                                                    ccTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTa                                             >  2:1330256/1‑139 (MQ=255)
                                                                     ctctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAg                                                                                    >  1:188892/1‑99 (MQ=255)
                                                                     ctctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAg                                                                                    <  2:188892/99‑1 (MQ=255)
                                                                       ctTCACATTTCGGGCAAATAATTGGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                  <  2:873375/131‑1 (MQ=255)
                                                                       ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                         <  1:1351940/76‑1 (MQ=255)
                                                                       ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                         >  2:1351940/1‑76 (MQ=255)
                                                                       ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                  <  1:212483/131‑1 (MQ=255)
                                                                       ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                  >  2:212483/1‑131 (MQ=255)
                                                                       ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTc                                          >  1:944207/1‑139 (MQ=255)
                                                                       ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCCTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                  >  1:873375/1‑131 (MQ=255)
                                                                        ttCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                         <  1:1482093/75‑1 (MQ=255)
                                                                        ttCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                         >  2:1482093/1‑75 (MQ=255)
                                                                               ttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                       >  1:979952/1‑118 (MQ=255)
                                                                               ttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                       <  2:979952/118‑1 (MQ=255)
                                                                                  gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGc                                                                  <  2:1057885/104‑1 (MQ=255)
                                                                                  gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGc                                                                  >  1:1057885/1‑104 (MQ=255)
                                                                                  gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCt                               >  1:1793325/1‑139 (MQ=255)
                                                                                          aatTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCg                       <  1:1358511/139‑1 (MQ=255)
                                                                                              cgcgAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACt                                                                                               >  2:1812714/1‑63 (MQ=255)
                                                                                              cgcgAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACt                                                                                               <  1:1812714/63‑1 (MQ=255)
                                                                                                  aaGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTtgat               <  2:1793325/139‑1 (MQ=255)
                                                                                                     aTAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc            <  1:1000967/139‑1 (MQ=255)
                                                                                                               aCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAg                                                                                    <  1:674541/57‑1 (MQ=255)
                                                                                                               aCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAg                                                                                    >  2:674541/1‑57 (MQ=255)
                                                                                                               aCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGaa                                                                               <  2:1320923/62‑1 (MQ=255)
                                                                                                               aCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGaa                                                                               >  1:1320923/1‑62 (MQ=255)
                                                                                                               aCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTa  >  2:1195052/1‑139 (MQ=255)
                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGAc                                                                                  >  2:312921/1‑55 (MQ=255)
                                                                                                                   aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGAc                                                                                  <  1:312921/55‑1 (MQ=255)
                                                                                                                        |                                                                                                                                  
GGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTA  >  NC_000913/1821740‑1821990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: