Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,214,339 (C)6→5 coding (306/1686 nt) yehU ← inner membrane putative sensory kinase in two‑component system with YehT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,214,3340C.98.2% 219.9 / ‑3.0 55coding (311/1686 nt)yehUinner membrane putative sensory kinase in two‑component system with YehT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base . (29/25);  minor base A (1/0);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAC  >  NC_000913/2214212‑2214468
                                                                                                                          |                                                                                                                                      
cACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATACCCCCATCGACTAACGa                                                                                                                       >  1:1348803/1‑139 (MQ=255)
   cggcggTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGt                                                                                                                    >  1:401984/1‑139 (MQ=255)
        gTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAgcc                                                                                                               >  1:518417/1‑139 (MQ=255)
             ggggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                          <  1:1164918/139‑1 (MQ=255)
             ggggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                          <  1:566462/139‑1 (MQ=255)
             ggggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                          <  2:401984/139‑1 (MQ=255)
                gTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCa                                                                                                       >  1:604213/1‑139 (MQ=255)
                                   cgcCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCGGCCCAACCAGCCCACcgac                                                                                    >  1:1336075/1‑139 (MQ=255)
                                     cccGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcgacga                                                                                  >  2:1215872/1‑139 (MQ=255)
                                     cccGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcgacga                                                                                  >  2:117651/1‑139 (MQ=255)
                                               aTCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGAccgcc                                                                        >  2:685093/1‑139 (MQ=255)
                                                      tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCGGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 <  2:1336075/139‑1 (MQ=255)
                                                         tgtgTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGc                                                                                                                                        >  2:1167310/1‑66 (MQ=255)
                                                         tgtgTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGc                                                                                                                                        <  1:1167310/66‑1 (MQ=255)
                                                         tgtgTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGa                                                                                                                              >  2:220790/1‑75 (MQ=255)
                                                         tgtgTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGa                                                                                                                              <  1:220790/75‑1 (MQ=255)
                                                                 aGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAc                                                      <  2:85482/139‑1 (MQ=255)
                                                                     cACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGccc                                                           >  2:1808773/1‑130 (MQ=255)
                                                                     cACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGccc                                                           <  1:1808773/130‑1 (MQ=255)
                                                                     cACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcgcg                                                  >  2:1508322/1‑139 (MQ=255)
                                                                       ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGcc                                                >  2:545148/1‑139 (MQ=255)
                                                                       ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGcc                                                <  2:604213/139‑1 (MQ=255)
                                                                       ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGcc                                                >  2:885232/1‑139 (MQ=255)
                                                                            tAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCg                                           <  2:829460/139‑1 (MQ=255)
                                                                              aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  1:1215872/139‑1 (MQ=255)
                                                                              aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  2:518417/139‑1 (MQ=255)
                                                                                 cTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                          >  2:189515/1‑71 (MQ=255)
                                                                                 cTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                          <  1:189515/71‑1 (MQ=255)
                                                                                    cAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTa                                   >  1:976781/1‑139 (MQ=255)
                                                                                          tGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCa                             <  1:685093/139‑1 (MQ=255)
                                                                                            gTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  1:763042/125‑1 (MQ=255)
                                                                                            gTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         >  2:763042/1‑125 (MQ=255)
                                                                                            gTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAAt                           >  2:1513512/1‑139 (MQ=255)
                                                                                             tCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGc                                          >  1:1193032/1‑123 (MQ=255)
                                                                                             tCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGc                                          <  2:1193032/123‑1 (MQ=255)
                                                                                                    cATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 <  1:1078749/93‑1 (MQ=255)
                                                                                                    cATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 >  2:1078749/1‑93 (MQ=255)
                                                                                                      tGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         >  2:1607133/1‑115 (MQ=255)
                                                                                                      tGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  1:1607133/115‑1 (MQ=255)
                                                                                                      tGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTc                  <  1:538165/138‑1 (MQ=255)
                                                                                                      tGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTc                  >  2:538165/1‑138 (MQ=255)
                                                                                                      tGCAACTTAACACGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa                 >  2:1302018/1‑139 (MQ=255)
                                                                                                       gCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCAcc                                                                                       <  1:209116/68‑1 (MQ=255)
                                                                                                       gCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCAcc                                                                                       >  2:209116/1‑68 (MQ=255)
                                                                                                           cTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcg                                                    >  2:217345/1‑99 (MQ=255)
                                                                                                           cTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcg                                                    <  1:217345/99‑1 (MQ=255)
                                                                                                            ttAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccggacc                                                                           <  1:1616589/75‑1 (MQ=255)
                                                                                                            ttAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccggacc                                                                           >  2:1616589/1‑75 (MQ=255)
                                                                                                            ttAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAAt                      <  2:265178/128‑1 (MQ=255)
                                                                                                            ttAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAAt                      >  1:265178/1‑128 (MQ=255)
                                                                                                               aCGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAAc        <  1:1513512/139‑1 (MQ=255)
                                                                                                               aCGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAAc        >  1:951678/1‑139 (MQ=255)
                                                                                                                cgcgGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAAt                      >  1:1741432/1‑124 (MQ=255)
                                                                                                                cgcgGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAAt                      <  2:1741432/124‑1 (MQ=255)
                                                                                                                     tCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGc                                                                                            <  2:1311665/49‑1 (MQ=255)
                                                                                                                     tCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGc                                                                                            >  1:1311665/1‑49 (MQ=255)
                                                                                                                     tCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAc  >  1:80313/1‑139 (MQ=255)
                                                                                                                          |                                                                                                                                      
CACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAC  >  NC_000913/2214212‑2214468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: