Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,012,553 Δ1 bp intergenic (+160/+61) yqeA → / ← yqeB putative amino acid kinase/XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,012,5520G.100.0% 268.2 / NA 69intergenic (+159/+62)yqeA/yqeBputative amino acid kinase/XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (35/34);  total (35/34)

TCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTAC  >  NC_000913/3012419‑3012685
                                                                                                                                     |                                                                                                                                     
tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAAtt                                                                                                                                 >  1:268141/1‑139 (MQ=255)
tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAAtt                                                                                                                                 >  2:649107/1‑139 (MQ=255)
tCCGGCCAAAGATCCTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGACGCGAGCCCCTTATCCTCCCTACAAAACATCGCAAATTCAATTGAGTGCAATGCACTTGTAGGCCTGGTAATCGAAGCGCATCAGCAAtt                                                                                                                                 >  1:1670928/1‑139 (MQ=25)
     ccAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCg                                                                                                                            <  2:493453/139‑1 (MQ=255)
      cAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGt                                                                                                                           <  2:1393064/139‑1 (MQ=255)
                      agtGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAg                                                                                                           >  1:1021962/1‑139 (MQ=255)
                           gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                      <  2:68501/139‑1 (MQ=255)
                           gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                      <  1:649107/139‑1 (MQ=255)
                           gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                      <  2:1400013/139‑1 (MQ=255)
                                gttgATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGtcat                                                                                                                    <  2:1662160/120‑1 (MQ=255)
                                gttgATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGtcat                                                                                                                    >  1:1662160/1‑120 (MQ=255)
                                      gCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                      >  2:244212/1‑128 (MQ=255)
                                      gCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                      <  1:244212/128‑1 (MQ=255)
                                       ccGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgggt                                                                                                    >  1:258932/1‑129 (MQ=255)
                                       ccGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgggt                                                                                                    <  2:258932/129‑1 (MQ=255)
                                                                   ccTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                      <  1:1256841/99‑1 (MQ=255)
                                                                   ccTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                      >  2:1256841/1‑99 (MQ=255)
                                                                    cTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGt                                                                                                                <  1:90619/88‑1 (MQ=255)
                                                                    cTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGt                                                                                                                >  2:90619/1‑88 (MQ=255)
                                                                    cTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTcc                                                             >  1:919574/1‑139 (MQ=255)
                                                                             aTCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCActtct                                                    >  1:1805278/1‑139 (MQ=255)
                                                                               cGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                      >  2:1560531/1‑87 (MQ=255)
                                                                               cGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                      <  1:1560531/87‑1 (MQ=255)
                                                                                 cAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGtcat                                                                                                                    <  2:11627/71‑1 (MQ=255)
                                                                                 cAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGtcat                                                                                                                    >  1:11627/1‑71 (MQ=255)
                                                                                 cAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTg                                                >  2:585993/1‑139 (MQ=255)
                                                                                          tAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                      <  1:156356/76‑1 (MQ=255)
                                                                                          tAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                      >  2:156356/1‑76 (MQ=255)
                                                                                              ttGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGcc                                   <  2:1805278/139‑1 (MQ=255)
                                                                                               tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAAtt                                                                                                                                 <  1:774281/44‑1 (MQ=17)
                                                                                               tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAAtt                                                                                                                                 >  2:774281/1‑44 (MQ=17)
                                                                                                gCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACg                                     >  2:720306/1‑135 (MQ=255)
                                                                                                gCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACg                                     <  1:720306/135‑1 (MQ=255)
                                                                                                    tGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATg                             <  2:919574/139‑1 (MQ=255)
                                                                                                      cACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgggt                                                                                                    <  1:33119/66‑1 (MQ=255)
                                                                                                      cACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgggt                                                                                                    >  2:33119/1‑66 (MQ=255)
                                                                                                      cACTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCa                           >  2:1252229/1‑139 (MQ=255)
                                                                                                       aCTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCAt                          <  2:312293/139‑1 (MQ=255)
                                                                                                       aCTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCAt                          >  2:978513/1‑139 (MQ=255)
                                                                                                       aCTTGTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCAt                          <  1:1252229/139‑1 (MQ=255)
                                                                                                           gTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGtt                                                                                                                          <  1:1084235/39‑1 (MQ=14)
                                                                                                           gTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGtt                                                                                                                          >  2:1084235/1‑39 (MQ=14)
                                                                                                           gTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCa                                       >  2:56957/1‑122 (MQ=255)
                                                                                                           gTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCa                                       <  1:56957/122‑1 (MQ=255)
                                                                                                           gTAGGCCTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAAc                      >  1:1748977/1‑139 (MQ=255)
                                                                                                                ccTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGc                                                                                                         >  2:1309733/1‑51 (MQ=17)
                                                                                                                ccTGATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGc                                                                                                         <  1:1309733/51‑1 (MQ=17)
                                                                                                                   gATAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTaa              >  2:1665708/1‑139 (MQ=255)
                                                                                                                    aTAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                      <  2:921868/50‑1 (MQ=18)
                                                                                                                    aTAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                      >  1:921868/1‑50 (MQ=18)
                                                                                                                     tAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGACCATTTTCAttt                                                                        >  2:1575835/1‑79 (MQ=37)
                                                                                                                     tAAGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGACCATTTTCAttt                                                                        <  1:1575835/79‑1 (MQ=37)
                                                                                                                      aaGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCAttt                                                                        <  2:1704202/78‑1 (MQ=255)
                                                                                                                      aaGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCAttt                                                                        >  1:1704202/1‑78 (MQ=255)
                                                                                                                      aaGCGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGc           <  2:532649/139‑1 (MQ=255)
                                                                                                                         cGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAg                                                                     >  2:264995/1‑78 (MQ=255)
                                                                                                                         cGTAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAg                                                                     <  1:264995/78‑1 (MQ=255)
                                                                                                                           tAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgggtggg                                                                                                 <  1:867751/48‑1 (MQ=25)
                                                                                                                           tAGCGCATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgggtggg                                                                                                 >  2:867751/1‑48 (MQ=25)
                                                                                                                              cgcATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                      >  1:1378209/1‑40 (MQ=21)
                                                                                                                              cgcATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                      <  2:1378209/40‑1 (MQ=21)
                                                                                                                              cgcATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCgg                                                                                    >  1:1407587/1‑58 (MQ=255)
                                                                                                                              cgcATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCgg                                                                                    <  2:1407587/58‑1 (MQ=255)
                                                                                                                              cgcATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGc                                                                    >  1:1298058/1‑74 (MQ=255)
                                                                                                                              cgcATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGc                                                                    <  2:1298058/74‑1 (MQ=255)
                                                                                                                              cgcATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGAt                              >  2:1434489/1‑112 (MQ=255)
                                                                                                                              cgcATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGAt                              <  1:1434489/112‑1 (MQ=255)
                                                                                                                              cgcATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTaa              <  1:1830608/128‑1 (MQ=255)
                                                                                                                              cgcATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTaa              >  2:1830608/1‑128 (MQ=255)
                                                                                                                               gcATCAGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGAGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTAc  >  1:1766830/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                     
TCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTAC  >  NC_000913/3012419‑3012685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: