Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,862,518 Δ1 bp pseudogene (1086/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,862,5160C.100.0% 350.9 / NA 84pseudogene (1088/1617 nt)glvCpseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (37/47);  total (37/47)

GAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGC  >  NC_000913/3862379‑3862647
                                                                                                                                         |                                                                                                                                   
gAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGcc                                                                                                                                    <  2:404938/139‑1 (MQ=255)
     aGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGtt                                                                                                                              <  1:1513212/139‑1 (MQ=255)
       tgtgCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTca                                                                                                                            <  1:756205‑M1/139‑3 (MQ=255)
        gtgCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcac                                                                                                                           >  2:161525‑M1/1‑136 (MQ=255)
        gtgCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcac                                                                                                                           <  1:161525‑M1/139‑4 (MQ=255)
          gCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcacca                                                                                                                         >  1:917347‑M1/1‑134 (MQ=255)
           cGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccac                                                                                                                        <  1:429544‑M1/139‑7 (MQ=255)
               aaaCCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacacca                                                                                                                    <  2:1520396‑M1/139‑11 (MQ=255)
               aaaCCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacacca                                                                                                                    <  1:160153‑M1/139‑11 (MQ=255)
                 aCCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaa                                                                                                                  <  2:911572‑M1/139‑13 (MQ=255)
                 aCCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaa                                                                                                                  <  1:1262174‑M1/139‑13 (MQ=255)
                 aCCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcacc                                                                                                                          >  1:1701472‑M1/1‑127 (MQ=255)
                 aCCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcacc                                                                                                                          <  2:1701472‑M1/131‑5 (MQ=255)
                  ccACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaag                                                                                                                 <  2:917347‑M1/139‑14 (MQ=255)
                                  ggTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcg                                                                                                 >  1:440252‑M1/1‑110 (MQ=255)
                                    tAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCTCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggt                                                                                                   >  1:1604794‑M1/1‑108 (MQ=255)
                                    tAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCTCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggt                                                                                                   <  2:1604794‑M1/135‑28 (MQ=255)
                                    tAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGccatg                                                                                                                                 >  2:880519/1‑103 (MQ=255)
                                    tAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGccatg                                                                                                                                 <  1:880519/106‑4 (MQ=255)
                                    tAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGtt                                                                                                                              >  2:23357/1‑108 (MQ=255)
                                    tAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGtt                                                                                                                              <  1:23357/108‑1 (MQ=255)
                                    tAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacgttacggtcgac                                                                                               >  2:1192963‑M1/1‑108 (MQ=255)
                                    tAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgac                                                                                               >  2:220867‑M1/1‑108 (MQ=255)
                                       aGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaa                                                                                                                  >  2:425877‑M1/1‑105 (MQ=255)
                                       aGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaa                                                                                                                  <  1:425877‑M1/117‑13 (MQ=255)
                                          acaACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgag                                                                                         <  1:629671‑M1/139‑38 (MQ=255)
                                            aaCCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGtt                                                                                                                              >  2:1345719/1‑100 (MQ=255)
                                            aaCCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGtt                                                                                                                              <  1:1345719/100‑1 (MQ=255)
                                            aaCCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaag                                                                                                                 >  2:853601‑M1/1‑100 (MQ=255)
                                            aaCCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaag                                                                                                                 <  1:853601‑M1/113‑14 (MQ=255)
                                            aaCCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacac                                                                                                                      <  1:947502‑M1/108‑9 (MQ=255)
                                            aaCCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGGCAATCAGACCTCCGCCATGTTcaccacac                                                                                                                      >  2:947502‑M1/1‑100 (MQ=255)
                                             aCCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGcc                                                                                                                                    >  2:711212/1‑94 (MQ=255)
                                             aCCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGcc                                                                                                                                    <  1:711212/94‑1 (MQ=255)
                                              cccAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGcc                                                                                                                                    >  2:850644/1‑93 (MQ=255)
                                              cccAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGcc                                                                                                                                    <  1:850644/93‑1 (MQ=255)
                                              cccAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccg                                                                                     >  2:1511298‑M1/1‑98 (MQ=255)
                                               ccAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgc                                                                                    <  1:1511298‑M1/139‑43 (MQ=255)
                                                   tGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGccat                                                                                                                                  >  1:905166/1‑88 (MQ=255)
                                                   tGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGccat                                                                                                                                  <  2:905166/90‑3 (MQ=255)
                                                   tGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagc                                                                                >  1:616944‑M1/1‑93 (MQ=255)
                                                    ggCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGCATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagca                                                                               <  1:1192963‑M1/139‑48 (MQ=255)
                                                      cGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacg                                                                             <  1:111223‑M1/139‑50 (MQ=255)
                                                        aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggc                                                                           >  2:635434‑M1/1‑88 (MQ=255)
                                                        aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggc                                                                           >  1:1801084‑M1/1‑88 (MQ=255)
                                                        aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcga                                                                                                <  2:1536862‑M1/118‑31 (MQ=255)
                                                        aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcga                                                                                                >  1:1536862‑M1/1‑88 (MQ=255)
                                                              gTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccg                                                                                     <  2:766286‑M1/123‑42 (MQ=255)
                                                              gTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccg                                                                                     >  1:766286‑M1/1‑82 (MQ=255)
                                                                   catcatATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaa                                                                >  2:1431083‑M1/1‑77 (MQ=255)
                                                                         atCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagca                                                          <  2:440252‑M1/139‑69 (MQ=255)
                                                                          tCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaa                                                         <  2:1221289‑M1/139‑70 (MQ=255)
                                                                            cgcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacg                                                       <  1:220867‑M1/139‑72 (MQ=255)
                                                                             gcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagc                                                                                >  1:378596‑M1/1‑67 (MQ=255)
                                                                             gcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagc                                                                                <  2:378596‑M1/113‑47 (MQ=255)
                                                                              cgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTCAAACTTAGTCAATCAGACCTCCGccat                                                                                                                                  <  1:1092183/63‑3 (MQ=255)
                                                                              cgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGccac                                                                                                                                  >  2:1092183/1‑61 (MQ=255)
                                                                              cgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggt                                                     <  2:616944‑M1/139‑74 (MQ=255)
                                                                                 ggTTGCTGAACATCGGGATCCAGTTTTGCGGTAAATCTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaa                                                  <  2:1153886‑M1/139‑77 (MQ=255)
                                                                                  gTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaa                                                  >  2:1073660‑M1/1‑62 (MQ=255)
                                                                                  gTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaa                                                  <  1:1073660‑M1/138‑77 (MQ=255)
                                                                                   ttGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacag                                                            <  2:794408‑M1/127‑67 (MQ=255)
                                                                                   ttGCTGAACATCGGGATCCAGTTTTGAGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacag                                                            >  1:794408‑M1/1‑61 (MQ=255)
                                                                                             tCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagca                                                          <  1:1809488‑M1/119‑69 (MQ=255)
                                                                                             tCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagca                                                          >  2:1809488‑M1/1‑51 (MQ=255)
                                                                                             tCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacag                                                            <  1:1714827‑M1/117‑67 (MQ=255)
                                                                                             tCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacag                                                            >  2:1714827‑M1/1‑51 (MQ=255)
                                                                                                ggATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGcc                                                                                                                                    <  2:1749572/43‑1 (MQ=255)
                                                                                                ggATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGcc                                                                                                                                    >  1:1749572/1‑43 (MQ=255)
                                                                                                ggATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgcctgcacggc                                                                           <  1:1202565‑M1/99‑52 (MQ=255)
                                                                                                ggATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggc                                                                           >  2:1202565‑M1/1‑48 (MQ=255)
                                                                                                   tCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTT‑‑‑CACCACACCaaa                                                                                                               <  1:1428382/57‑1 (MQ=255)
                                                                                                   tCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTT‑‑‑CACCACACCaaa                                                                                                               >  2:1428382/1‑57 (MQ=255)
                                                                                                        ttttGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaaggtaaattccag                           >  1:283317‑M1/1‑40 (MQ=255)
                                                                                                        ttttGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaaggtaaattccag                           <  1:1431083‑M1/139‑100 (MQ=255)
                                                                                                        ttttGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacg                                                       <  1:757313‑M1/111‑72 (MQ=255)
                                                                                                        ttttGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacg                                                       >  2:757313‑M1/1‑40 (MQ=255)
                                                                                                             cGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggc                                                                                       >  2:332943‑M1/1‑35 (MQ=255)
                                                                                                             cGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggc                                                                                       <  1:332943‑M1/74‑40 (MQ=255)
                                                                                                             cGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagagacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaaggtaaattccagcggtt                      >  1:1132126‑M1/1‑35 (MQ=255)
                                                                                                               gTAAAACTTAGTCAATCAGACCTCCGCCATGTT‑‑‑CACCACACCAAAGAGATACa                                                                                                       <  2:885782/53‑1 (MQ=255)
                                                                                                               gTAAAACTTAGTCAATCAGACCTCCGCCATGTT‑‑‑CACCACACCAAAGAGATACa                                                                                                       >  1:885782/1‑53 (MQ=255)
                                                                                                                  aaaCTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggc                                                                           <  1:411355‑M1/81‑52 (MQ=255)
                                                                                                                  aaaCTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggc                                                                           >  2:411355‑M1/1‑30 (MQ=255)
                                                                                                                   aacttagtcAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaaggtaaattccagcggttcggtaa                <  2:283317‑M1/135‑111 (MQ=255)
                                                                                                                        aGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagc                                                                                >  2:788282‑M1/1‑24 (MQ=255)
                                                                                                                        aGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagc                                                                                <  1:788282‑M1/70‑47 (MQ=255)
                                                                                                                          tCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacagg                                         <  2:1625033‑M1/107‑86 (MQ=255)
                                                                                                                          tCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacagg                                         >  1:1625033‑M1/1‑22 (MQ=255)
                                                                                                                          tCAATCAGACCTCCGCCATGTT‑‑‑CACCACACCAAAgaga                                                                                                           >  2:773404/1‑38 (MQ=255)
                                                                                                                          tCAATCAGACCTCCGCCATGTT‑‑‑CACCACACCAAAgaga                                                                                                           <  1:773404/38‑1 (MQ=12)
                                                                                                                              tCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaa                                             <  2:891062‑M1/99‑82 (MQ=255)
                                                                                                                              tCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaa                                             >  1:891062‑M1/1‑18 (MQ=255)
                                                                                                                               cAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaaggtaaattccagcggttcggtaattcccaccagca    <  1:635434‑M1/139‑123 (MQ=255)
                                                                                                                                aGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgcc                                                                                   <  2:1734888‑M1/59‑44 (MQ=255)
                                                                                                                                aGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgcc                                                                                   >  1:1734888‑M1/1‑16 (MQ=255)
                                                                                                                                 gACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagctcggcgtgtaccgcaaacagcaacggtgaaatgaacaggaaggtaaattccagcggttcggtaattcccaccagcatg  <  2:1132126‑M1/139‑125 (MQ=255)
                                                                                                                                         |                                                                                                                                   
GAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGC  >  NC_000913/3862379‑3862647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: