Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,250,629	(C)5→6	coding (210/1071 nt)	dhaK ←	dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,250,624	1	.	C	100.0%	203.6 / NA	59	A72G (GCC→GGC)	dhaK	dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (28/31);  total (28/31)

TCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATG  >  NC_000913/1250486‑1250754                                                                                                                                            |                                                                                                                                   tCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAgg                                                                                                                                     <  2:402017/139‑1 (MQ=255)   ggTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑cc                                                                                                                                  >  1:1203696/1‑139 (MQ=255)       gCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑cccccc                                                                                                                              >  1:1002380/1‑138 (MQ=255)             tCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGc                                                                                                                         <  1:77941/139‑1 (MQ=255)                       gAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGAc                                                                                                               <  1:482479/139‑1 (MQ=255)                       gAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGAc                                                                                                               <  2:1638654/139‑1 (MQ=255)                        aaTATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGAcc                                                                                                              <  2:447204/139‑1 (MQ=255)                             cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGatat                                                                                                         <  1:574189/139‑1 (MQ=255)                             cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑c                                                                                                                                   <  1:1557249/112‑1 (MQ=255)                             cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑c                                                                                                                                   >  2:1557249/1‑112 (MQ=255)                               ccGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATaa                                                                                                       >  1:361708/1‑139 (MQ=255)                                  gtgtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACca                                                                                                    <  2:45075/139‑1 (MQ=255)                                    gtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACcaca                                                                                                  <  1:1188442/139‑1 (MQ=255)                                    gtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACcaca                                                                                                  <  2:361708/139‑1 (MQ=255)                                                  tCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCgg                                                                                         >  1:980909/1‑134 (MQ=255)                                                  tCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCgg                                                                                         <  2:980909/134‑1 (MQ=255)                                                           cacCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgc                                                                           <  2:922543/139‑1 (MQ=255)                                                            acCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCAT‑‑CCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc                                                                        >  1:890570/1‑139 (MQ=255)                                                              cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc                                                                        >  2:1522675/1‑139 (MQ=255)                                                              cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc                                                                        <  1:1522675/139‑1 (MQ=255)                                                              cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTG‑AAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgcc                                                                       >  2:764346/1‑139 (MQ=255)                                                                tCGCCGCCATCAACTTGCATTGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCa                                                                      >  1:1080269/1‑139 (MQ=255)                                                                 cgccgcCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGCTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCAc                                                                     >  1:1318586/1‑139 (MQ=255)                                                                   ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGGAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGAc                                                                                                               >  2:667671/1‑95 (MQ=255)                                                                   ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGGAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGAc                                                                                                               <  1:667671/95‑1 (MQ=255)                                                                   ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCAccc                                                                   <  2:809516/139‑1 (MQ=255)                                                                        aTCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACcaca                                                                                                  >  1:1107848/1‑103 (MQ=255)                                                                        aTCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACcaca                                                                                                  <  2:1107848/103‑1 (MQ=255)                                                                        aTCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCgg                                                                                         >  1:1640738/1‑112 (MQ=255)                                                                        aTCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCgg                                                                                         <  2:1640738/112‑1 (MQ=255)                                                                                 cATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑c                                                                                                                                   <  1:615847/60‑1 (MQ=255)                                                                                 cATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑c                                                                                                                                   >  2:615847/1‑60 (MQ=255)                                                                                     gcgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACtttt                                                 >  2:450722/1‑139 (MQ=255)                                                                                      cgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc                                                                        <  2:1025649/115‑1 (MQ=255)                                                                                      cgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc                                                                        >  1:1025649/1‑115 (MQ=255)                                                                                      cgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTc                                                >  1:1021419/1‑139 (MQ=255)                                                                                      cgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTc                                                >  2:26137/1‑139 (MQ=255)                                                                                           tCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCa                                           >  1:1273668/1‑139 (MQ=255)                                                                                             aaaGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAAc                                         <  1:1676123/139‑1 (MQ=255)                                                                                                   tttATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGc                                   <  1:450722/139‑1 (MQ=255)                                                                                                     tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATCTAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt                                 >  1:866802/1‑139 (MQ=255)                                                                                                     tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACca                                                                                                    <  2:1019180/72‑1 (MQ=255)                                                                                                     tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACca                                                                                                    >  1:1019180/1‑72 (MQ=255)                                                                                                     tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGGATCGGCTCGTGTCCGCTGCCGCCCCCCGACAGCATGGCGACTTTTCCTGCAACAGGGGCAt                                 >  1:1168211/1‑139 (MQ=255)                                                                                                     tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc                                                                        <  2:103778/100‑1 (MQ=255)                                                                                                     tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc                                                                        >  1:103778/1‑100 (MQ=255)                                                                                                     tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc                                                                        <  1:317342/100‑1 (MQ=255)                                                                                                     tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc                                                                        >  2:317342/1‑100 (MQ=255)                                                                                                     tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt                                 >  1:238614/1‑139 (MQ=255)                                                                                                         gggCGTCGGTGAGGTGAAAATATCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGc                             <  2:1080269/139‑1 (MQ=255)                                                                                                             gTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCgg                         <  2:238614/139‑1 (MQ=255)                                                                                                              tCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCggg                        >  2:1547633/1‑139 (MQ=255)                                                                                                                ggtgaggtgaAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTg                      <  1:26137/139‑1 (MQ=255)                                                                                                                     ggtgaAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCAt                                                                                            >  2:279666/1‑64 (MQ=255)                                                                                                                     ggtgaAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCAt                                                                                            <  1:279666/64‑1 (MQ=255)                                                                                                                      gtgaAAATTTCGCCCGGACAGGCCCCCCGAAAGCa                                                                                                                        >  2:327571/1‑35 (MQ=255)                                                                                                                      gtgaAAATTTCGCCCGGACAGGCCCCCCGAAAGCa                                                                                                                        <  1:327571/35‑1 (MQ=255)                                                                                                                      gtgaAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATac                >  1:1146217/1‑139 (MQ=255)                                                                                                                          aaaTTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCACGGCGACTTTTTCTGCAACAGGGGCATCAGCTCGGGTGACATACACCg            <  2:1168211/139‑1 (MQ=255)                                                                                                                               tCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCAt                                                                                            <  2:383449/54‑1 (MQ=255)                                                                                                                               tCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCAt                                                                                            >  1:383449/1‑54 (MQ=255)                                                                                                                                cGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGatat                                                                                                         >  2:742448/1‑40 (MQ=255)                                                                                                                                cGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGatat                                                                                                         <  1:742448/40‑1 (MQ=255)                                                                                                                                    cGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTACCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATg  <  2:1318586/139‑1 (MQ=255)                                                                                                                                     ggACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACa                                        <  2:1385969/100‑1 (MQ=255)                                                                                                                                     ggACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACa                                        >  1:1385969/1‑100 (MQ=255)                                                                                                                                            |                                                                                                                                   TCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATG  >  NC_000913/1250486‑1250754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑