Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,769,478	(G)7→8	coding (405/1113 nt)	ydiK →	UPF0118 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,769,471	1	.	G	100.0%	147.4 / NA	43	M133R (ATG→AGG)	ydiK	UPF0118 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (18/25);  total (18/25)

GTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTG  >  NC_000913/1769336‑1769596                                                                                                                                         |                                                                                                                                gTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑‑ggg                                                                                                                            >  2:858915/1‑139 (MQ=255)    tcgtcgACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑‑gggggg                                                                                                                         <  1:1588671/139‑1 (MQ=255)       tcgACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑‑gggggggga                                                                                                                      >  2:1727158/1‑137 (MQ=255)       tcgACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑‑gggggggga                                                                                                                      >  1:148558/1‑137 (MQ=255)                          aaTTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGt                                                                                                    <  2:152616/139‑1 (MQ=255)                           aTTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTc                                                                                                   <  1:1054687/139‑1 (MQ=255)                            ttAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTcc                                                                                                  <  1:906414/139‑1 (MQ=255)                                     tttCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTtata                                                                                         >  2:134469/1‑139 (MQ=255)                                     tttCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑‑gggg                                                                                                                           >  1:1491636/1‑104 (MQ=255)                                     tttCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑‑gggg                                                                                                                           <  2:1491636/104‑1 (MQ=255)                                         cAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTgg                                                                                     <  1:293335/139‑1 (MQ=255)                                                 aCATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcac                                                                                  <  1:1660950/134‑1 (MQ=255)                                                 aCATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcac                                                                                  >  2:1660950/1‑134 (MQ=255)                                                           aCCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCggg                                                                     >  1:1781028/1‑139 (MQ=255)                                                           aCCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATAtt                                                                                       <  2:1273432/120‑1 (MQ=255)                                                           aCCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATAtt                                                                                       >  1:1273432/1‑120 (MQ=255)                                                           aCCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGtt                                                                   >  2:492499/1‑139 (MQ=255)                                                                     gCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAg                                                         <  1:492499/139‑1 (MQ=255)                                                                     gCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAg                                                         <  1:499732/139‑1 (MQ=255)                                                                     gCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATACG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCGCCTGGTTCGTTGGGCAg                                                         <  1:1050072/139‑1 (MQ=255)                                                                          gCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCg                                                                                                 >  1:665001/1‑94 (MQ=255)                                                                          gCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCg                                                                                                 <  2:665001/94‑1 (MQ=255)                                                                             tAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCa                                                 >  2:745752/1‑139 (MQ=255)                                                                                    aTTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTcc                                                                                                  <  1:1085620/83‑1 (MQ=255)                                                                                    aTTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTcc                                                                                                  >  2:1085620/1‑83 (MQ=255)                                                                                    aTTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCggg                                          <  1:858915/139‑1 (MQ=255)                                                                                     ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTgg                                                                                     <  2:707625/95‑1 (MQ=255)                                                                                     ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTgg                                                                                     >  1:707625/1‑95 (MQ=255)                                                                                        cGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCt                                      <  1:1727158/139‑1 (MQ=255)                                                                                        cGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCt                                      <  1:134469/139‑1 (MQ=255)                                                                                          gTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCt                                      <  1:1333118/137‑1 (MQ=255)                                                                                          gTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCt                                      >  2:1333118/1‑137 (MQ=255)                                                                                            gATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTAt                                  <  2:148558/139‑1 (MQ=255)                                                                                             aTTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATg                                 >  2:593518/1‑139 (MQ=255)                                                                                                   gcgAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCTCCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCTCTTTATGGTGCAt                           <  1:168001/139‑1 (MQ=255)                                                                                                            tATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTg                  >  2:556972/1‑139 (MQ=255)                                                                                                                      ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGt                                                                                                    <  1:1137506/47‑1 (MQ=255)                                                                                                                      ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGt                                                                                                    >  2:1137506/1‑47 (MQ=255)                                                                                                                      ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTc                                                                                                   >  2:609619/1‑48 (MQ=255)                                                                                                                      ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTc                                                                                                   >  2:670872/1‑48 (MQ=255)                                                                                                                      ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTc                                                                                                   <  1:609619/48‑1 (MQ=39)                                                                                                                      ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTc                                                                                                   <  1:670872/48‑1 (MQ=39)                                                                                                                      ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcaccaccaccac                                                                         >  2:274646/1‑74 (MQ=255)                                                                                                                      ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcaccaccaccac                                                                         <  1:274646/74‑1 (MQ=255)                                                                                                                      ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgcg                                        >  2:1024466/1‑107 (MQ=255)                                                                                                                      ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgcg                                        <  1:1024466/107‑1 (MQ=255)                                                                                                                      ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTctct        >  2:509983/1‑139 (MQ=255)                                                                                                                            aCTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTg  <  1:556972/139‑1 (MQ=255)                                                                                                                            aCTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTg  <  1:593518/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                GTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTG  >  NC_000913/1769336‑1769596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑