Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,240,754 (C)5→6 coding (915/1041 nt) galS ← galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,240,7491.C97.1% 232.7 / ‑2.8 70A307G (GCA→GGA) galSgalactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor
Reads supporting (aligned to +/- strand):  ref base . (0/1);  major base C (33/34);  minor base T (1/1);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.06e-01

CACAGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAAT  >  NC_000913/2240612‑2240882
                                                                                                                                          |                                                                                                                                      
cacaGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATCAAACAGTGGCTGGCACGAGGATCAATATTGACtgctgc                                                                                                                                       >  1:539772/1‑139 (MQ=255)
 acaGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGcc                                                                                                                                      <  1:886550/139‑1 (MQ=255)
       tGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGcccccctg                                                                                                                               <  1:1203518/139‑4 (MQ=255)
         aaaGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGcccccctgca                                                                                                                             >  1:1416823/1‑134 (MQ=255)
           cgCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAg                                                                                                                            >  1:1643594/2‑139 (MQ=255)
               gTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCa                                                                                                                        >  1:1589741/1‑139 (MQ=255)
                    aTCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCg                                                                                                                   <  1:1014710/139‑1 (MQ=255)
                    aTCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCg                                                                                                                   >  1:860352/1‑139 (MQ=255)
                    aTCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCg                                                                                                                   <  1:606665/139‑1 (MQ=255)
                    aTCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCg                                                                                                                   <  1:1599439/139‑1 (MQ=255)
                        ccTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGtt                                                                                                                     >  2:168298/1‑133 (MQ=255)
                        ccTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGtt                                                                                                                     <  1:168298/133‑1 (MQ=255)
                        ccTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTgg                                                                                                               >  2:636229/1‑139 (MQ=255)
                                      ttAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCAtt                                                                                                 >  2:1021412/1‑139 (MQ=255)
                                        aGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa                                                                                               <  2:982958/139‑1 (MQ=255)
                                              cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAAttt                                                                                                        <  1:953138/124‑1 (MQ=255)
                                              cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAAttt                                                                                                        >  2:953138/1‑124 (MQ=255)
                                              cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAg                                                                                             >  1:1577850/1‑135 (MQ=255)
                                              cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAg                                                                                             <  2:1577850/135‑1 (MQ=255)
                                              cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGccc                                                                                                                                     >  2:609179/1‑95 (MQ=255)
                                              cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGccc                                                                                                                                     <  1:609179/95‑1 (MQ=255)
                                               gccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCctgctg                                                                                                                                         >  1:951711/1‑91 (MQ=255)
                                               gccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCctgctg                                                                                                                                         <  2:951711/91‑1 (MQ=255)
                                                ccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATgg                                                                                       >  2:960862/1‑139 (MQ=255)
                                                         tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                              <  2:539772/139‑1 (MQ=255)
                                                                tgtTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTc                                                                                                       >  2:429036/1‑107 (MQ=255)
                                                                tgtTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTc                                                                                                       <  1:429036/107‑1 (MQ=255)
                                                                tgtTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                              <  1:759445/132‑1 (MQ=255)
                                                                tgtTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                              >  2:759445/1‑132 (MQ=255)
                                                                  ttGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGTCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa                                                                                          <  2:235654/118‑1 (MQ=255)
                                                                  ttGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGTCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa                                                                                          >  1:235654/1‑118 (MQ=255)
                                                                       aCAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                              <  2:1739307/125‑1 (MQ=255)
                                                                       aCAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                              >  1:1739307/1‑125 (MQ=255)
                                                                          gAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTc                                                                                                       <  2:868397/97‑1 (MQ=255)
                                                                          gAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTc                                                                                                       >  1:868397/1‑97 (MQ=255)
                                                                             tGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTg                                                                                                <  1:397419/101‑1 (MQ=255)
                                                                             tGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTg                                                                                                >  2:397419/1‑101 (MQ=255)
                                                                                  aCGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGc                                                                                                                          >  1:424293/1‑70 (MQ=255)
                                                                                  aCGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGc                                                                                                                          <  2:424293/70‑1 (MQ=255)
                                                                                  aCGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCg                                                                                                                   >  1:474878/1‑77 (MQ=255)
                                                                                  aCGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCg                                                                                                                   >  1:1336696/1‑77 (MQ=255)
                                                                                  aCGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCg                                                                                                                   <  2:1336696/77‑1 (MQ=255)
                                                                                  aCGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCg                                                                                                                   <  2:474878/77‑1 (MQ=255)
                                                                                     cACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAGTATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCa                                                                                                   >  2:1088349/1‑90 (MQ=255)
                                                                                     cACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAGTATTGCCTGCTGCCCCTCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCa                                                                                                   <  1:1088349/90‑1 (MQ=255)
                                                                                     cACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACg                                                  >  1:1806784/1‑139 (MQ=255)
                                                                                     cACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACg                                                  >  1:1725638/1‑139 (MQ=255)
                                                                                       cTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACggg                                                <  1:960862/139‑1 (MQ=255)
                                                                                        tAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGc                                               <  2:860352/139‑1 (MQ=255)
                                                                                         aaCGTCGGCATAAAACAGTGGCTGGCACGAGTATCAATATTGCCTGCTGccc                                                                                                                                     <  1:1079856/52‑1 (MQ=255)
                                                                                         aaCGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGccc                                                                                                                                     >  2:1079856/1‑52 (MQ=255)
                                                                                          aCGTGGGCATAAAACAGTGGGTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCACTGAAGCAATGGGATAACGCACGGTCGTTAATTGAGGGTCGGTGTAACGGGCGa                                             >  1:1076876/1‑139 (MQ=255)
                                                                                           cGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGAt                                            <  2:1416823/139‑1 (MQ=255)
                                                                                                gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCgg                                                              >  2:616869/1‑116 (MQ=255)
                                                                                                gCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCgg                                                              <  1:616869/116‑1 (MQ=255)
                                                                                                        cAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCg                               <  2:116344/139‑1 (MQ=255)
                                                                                                           tggctggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa                                                                                               >  2:1525810/1‑72 (MQ=255)
                                                                                                           tggctggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa                                                                                               <  1:1525810/72‑1 (MQ=255)
                                                                                                           tggctggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                              <  2:521476/89‑1 (MQ=255)
                                                                                                           tggctggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                              >  1:521476/1‑89 (MQ=255)
                                                                                                           tggctggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGaaa                            >  1:115837/1‑139 (MQ=255)
                                                                                                              ctggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAAt                                                                                                          >  2:1541716/1‑58 (MQ=255)
                                                                                                              ctggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAAt                                                                                                          <  1:1541716/58‑1 (MQ=255)
                                                                                                              ctggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCg                         <  1:1012267/139‑1 (MQ=255)
                                                                                                                ggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACg                                                                                >  1:561127/1‑82 (MQ=255)
                                                                                                                ggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACg                                                                                <  2:561127/82‑1 (MQ=255)
                                                                                                                ggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGc                                               <  1:1377052/115‑1 (MQ=255)
                                                                                                                ggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGc                                               >  2:1377052/1‑115 (MQ=255)
                                                                                                                ggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCgat                       >  1:1036538/1‑139 (MQ=255)
                                                                                                                       ggATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgag                <  2:1036538/139‑1 (MQ=255)
                                                                                                                       ggATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgag                <  1:1021412/139‑1 (MQ=255)
                                                                                                                       ggATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgag                <  1:636229/139‑1 (MQ=255)
                                                                                                                        gATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgaga               <  2:114727/139‑1 (MQ=255)
                                                                                                                                 tGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGc                                                                                                     >  1:94697/1‑44 (MQ=255)
                                                                                                                                 tGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGc                                                                                                     <  2:94697/44‑1 (MQ=255)
                                                                                                                                  gCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                              <  2:456472/66‑1 (MQ=255)
                                                                                                                                  gCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                              >  1:456472/1‑66 (MQ=255)
                                                                                                                                  gCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACgg     >  2:1670915/1‑139 (MQ=255)
                                                                                                                                   ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCga                        >  2:1387711/1‑119 (MQ=255)
                                                                                                                                   ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCga                        <  1:1387711/119‑1 (MQ=255)
                                                                                                                                     tgctgcCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCg                               >  1:176742/1‑110 (MQ=255)
                                                                                                                                     tgctgcCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCg                               <  2:176742/110‑1 (MQ=255)
                                                                                                                                     tgctgcCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgag                >  2:821027/1‑125 (MQ=255)
                                                                                                                                     tgctgcCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAAt  >  1:288917/1‑139 (MQ=255)
                                                                                                                                     tgctgcCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGAGCGATGGGAATATCATCGAAACCGATGATTgag                <  1:821027/125‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                      
CACAGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGAAT  >  NC_000913/2240612‑2240882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: