Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,330,357 +T coding (1395/1434 nt) dacB → D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,330,3531.T100.0% 230.0 / NA 72R464L (CGT→CTT) dacBD‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (39/33);  total (39/33)

GGTTCGTTGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCC  >  NC_000913/3330217‑3330486
                                                                                                                                         |                                                                                                                                     
ggTTCGTTGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑tt                                                                                                                                     >  2:270045/1‑139 (MQ=255)
       tGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑tttttgaaa                                                                                                                              <  1:1240491/139‑6 (MQ=255)
       tGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑tttttgaaa                                                                                                                              <  1:645799/139‑6 (MQ=255)
       tGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑tttttgaaa                                                                                                                              <  1:1388939/139‑6 (MQ=255)
        gCAGGGGGTATATAACCTGGCGGGATTCATTACCAC‑GCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGc                                                                                                                             <  2:629059/139‑1 (MQ=255)
            ggggTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                                                          <  2:543271/139‑1 (MQ=255)
                 atataACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGta                                                                                                                     <  1:1007887/139‑1 (MQ=255)
                      aCCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                                                          >  2:1381417/1‑129 (MQ=255)
                      aCCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAAGATCTTTCTGGCTAAGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                                                          <  1:1381417/129‑1 (MQ=255)
                        cTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGc                                                                                                                             >  1:725450/1‑124 (MQ=255)
                        cTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGc                                                                                                                             <  2:725450/124‑1 (MQ=255)
                              ggATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                                                          >  2:1289280/1‑121 (MQ=255)
                              ggATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                                                          <  1:1289280/121‑1 (MQ=255)
                                 ttCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGtt                                                                                                                         <  2:1821339/119‑1 (MQ=255)
                                 ttCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGtt                                                                                                                         >  1:1821339/1‑119 (MQ=255)
                                           caGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTaat                                                                                           >  2:1452268/1‑137 (MQ=255)
                                            agcgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTaatc                                                                                          <  1:1596002/139‑4 (MQ=255)
                                             gcgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGc                                                                                                                             <  2:1812143/103‑1 (MQ=255)
                                             gcgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGc                                                                                                                             >  1:1812143/1‑103 (MQ=255)
                                              cgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCg                                                                                                                           >  2:628261/1‑104 (MQ=255)
                                              cgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCg                                                                                                                           <  1:628261/104‑1 (MQ=255)
                                              cgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGttt                                                                                                                        <  2:871599/107‑1 (MQ=255)
                                              cgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGttt                                                                                                                        >  1:871599/1‑107 (MQ=255)
                                              cgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATaa                                                                                                                  <  1:1270345/113‑1 (MQ=255)
                                              cgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATaa                                                                                                                  >  2:1270345/1‑113 (MQ=255)
                                              cgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGata                                                                                                             >  2:717189/1‑118 (MQ=255)
                                              cgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGata                                                                                                             <  1:717189/118‑1 (MQ=255)
                                              cgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCaa                                                                                        >  1:901004/1‑139 (MQ=255)
                                              cgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑tttttgaa                                                                                                                               <  1:510673/99‑5 (MQ=255)
                                              cgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑tttttgaa                                                                                                                               >  2:510673/1‑95 (MQ=255)
                                                agcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCaaaa                                                                                      >  1:594924/1‑139 (MQ=255)
                                                agcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCaaaa                                                                                      >  2:888143/1‑139 (MQ=255)
                                                   gggCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGa                                                                                   <  1:1452268/139‑1 (MQ=255)
                                                                    tgtgCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGGTCAGCGTAATCGCCGTATTATGTTAGTGCGTTTTTGAAAGCCGTTTGTATaa                                                                                                                  <  2:375214/91‑1 (MQ=255)
                                                                    tgtgCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATaa                                                                                                                  >  1:375214/1‑91 (MQ=255)
                                                                          atatCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGc                                                                                                                             <  1:1686579/74‑1 (MQ=255)
                                                                          atatCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGc                                                                                                                             >  2:1686579/1‑74 (MQ=255)
                                                                                ttCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                                                          <  2:1828451/71‑1 (MQ=255)
                                                                                ttCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                                                          >  1:1828451/1‑71 (MQ=255)
                                                                                ttCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCaaaaa                                                                                     >  1:1039154/1‑108 (MQ=255)
                                                                                ttCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCaaaaa                                                                                     <  2:1039154/108‑1 (MQ=255)
                                                                                ttCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCgg                                                                           >  2:1482984/1‑118 (MQ=255)
                                                                                ttCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCgg                                                                           <  1:1482984/118‑1 (MQ=255)
                                                                                     gCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATaa                                                                                                                  >  2:210312/1‑74 (MQ=255)
                                                                                     gCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATaa                                                                                                                  <  1:210312/74‑1 (MQ=255)
                                                                                     gCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTg                                                 >  1:1258093/1‑139 (MQ=255)
                                                                                       tATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                                                          <  2:115871/64‑1 (MQ=255)
                                                                                       tATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGt                                                                                                                          >  1:115871/1‑64 (MQ=255)
                                                                                       tATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGa                                                                                                               <  1:1354097/75‑1 (MQ=255)
                                                                                       tATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGa                                                                                                               >  2:1354097/1‑75 (MQ=255)
                                                                                        aTGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACa                                              >  2:1747313/1‑139 (MQ=255)
                                                                                           cAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGa                                                                                   >  2:1570592/1‑99 (MQ=255)
                                                                                           cAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGa                                                                                   <  1:1570592/99‑1 (MQ=255)
                                                                                           cAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAg                                           >  1:1437611/1‑139 (MQ=255)
                                                                                               aGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGata                                                                                                             <  2:1274999/69‑1 (MQ=255)
                                                                                               aGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGata                                                                                                             >  1:1274999/1‑69 (MQ=255)
                                                                                                gAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTgcgc                                      <  1:888143/139‑1 (MQ=255)
                                                                                                   ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac                                                                        >  2:257495/1‑102 (MQ=255)
                                                                                                   ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac                                                                        <  1:257495/102‑1 (MQ=255)
                                                                                                   ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCttt                                   >  2:802310/1‑139 (MQ=255)
                                                                                                    cTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTg                                  >  2:503144/1‑139 (MQ=255)
                                                                                                      gCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGtt                                >  2:925630/1‑139 (MQ=255)
                                                                                                       cGGATCAGCGTAATCGCCGTATTCCGTTAGTGTGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGttt                               >  2:1767682/1‑139 (MQ=255)
                                                                                                       cGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGttt                               >  1:278058/1‑139 (MQ=255)
                                                                                                        ggATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGata                                                                                                             <  1:670014/60‑1 (MQ=255)
                                                                                                        ggATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGata                                                                                                             >  2:670014/1‑60 (MQ=255)
                                                                                                               cGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAg                                                                                                     <  1:606156/61‑1 (MQ=255)
                                                                                                               cGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAg                                                                                                     >  2:606156/1‑61 (MQ=255)
                                                                                                                    tCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTAt                                                                                                        >  1:1104174/1‑53 (MQ=255)
                                                                                                                    tCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTAt                                                                                                        <  2:1104174/53‑1 (MQ=255)
                                                                                                                    tCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCg                                                                 >  2:1203011/1‑92 (MQ=255)
                                                                                                                    tCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCg                                                                 <  1:1203011/92‑1 (MQ=255)
                                                                                                                         gTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa             <  1:503144/139‑1 (MQ=255)
                                                                                                                         gTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa             >  2:1750047/1‑139 (MQ=255)
                                                                                                                                  tAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGat                                                      <  1:1522785/89‑1 (MQ=255)
                                                                                                                                  tAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGat                                                      >  2:1522785/1‑89 (MQ=255)
                                                                                                                                   aGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc   <  1:802310/139‑1 (MQ=255)
                                                                                                                                    gTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATcc  >  1:1365939/1‑139 (MQ=255)
                                                                                                                                         |                                                                                                                                     
GGTTCGTTGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCC  >  NC_000913/3330217‑3330486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑