Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,980,957 (G)6→7 coding (71/1386 nt) yifK → putative APC family amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,980,9511.G100.0% 302.1 / NA 85L22R (CTG→CGG) yifKputative APC family amino acid transporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (43/42);  total (43/42)

CTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAA  >  NC_000913/3980824‑3981082
                                                                                                                                |                                                                                                                                   
cTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTggggggg                                                                                                                              <  1:707216/135‑2 (MQ=255)
cTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTggggggg                                                                                                                              >  2:707216/1‑134 (MQ=255)
   gATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCAtt                                                                                                                        >  1:323395/1‑139 (MQ=255)
       tACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGTTAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCg                                                                                                                    <  2:1806688/139‑1 (MQ=255)
        aCCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGt                                                                                                                   >  2:1582569/1‑139 (MQ=255)
        aCCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGt                                                                                                                   <  1:1582569/139‑1 (MQ=255)
        aCCCATAACGATAACCGGAAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGt                                                                                                                   >  2:1691954/1‑139 (MQ=255)
          ccATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCg                                                                                                                 <  2:1722999/139‑1 (MQ=255)
             tAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGcc                                                                                                              >  1:1027361/1‑139 (MQ=255)
             tAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTggggggg                                                                                                                              >  2:407077/1‑121 (MQ=255)
             tAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTggggggg                                                                                                                              <  1:407077/122‑2 (MQ=255)
              aaCGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCt                                                                                                             >  1:1079474/1‑139 (MQ=255)
               aCGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTg                                                                                                            >  2:34187/1‑139 (MQ=255)
                   tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCg                                                                                                                    >  2:761360/1‑127 (MQ=255)
                   tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCg                                                                                                                    <  1:761360/127‑1 (MQ=255)
                   tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCgg                                                                                                                >  1:121650/1‑131 (MQ=255)
                   tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCgg                                                                                                                <  2:121650/131‑1 (MQ=255)
                   tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTa                                                                                                        >  2:107532/1‑139 (MQ=255)
                    aaCCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTAt                                                                                                       >  2:446378/1‑139 (MQ=255)
                     aCCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTggg                                                                                                                                  >  2:409514/1‑110 (MQ=255)
                     aCCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTggg                                                                                                                                  <  1:409514/110‑1 (MQ=255)
                      ccGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGTGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATgg                                                                                                     >  1:978486/1‑139 (MQ=255)
                      ccGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATgg                                                                                                     <  1:1672629/139‑1 (MQ=255)
                        ggTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATgggg                                                                                                   <  2:323395/139‑1 (MQ=255)
                         gTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGAGTCGGCCTGTTTATggggg                                                                                                  >  2:822803/1‑139 (MQ=255)
                           aaCACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgcc                                                                                                >  1:783620/1‑139 (MQ=255)
                               ccGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGTGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa                                                                                            <  2:978486/139‑1 (MQ=255)
                               ccGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTGGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa                                                                                            <  1:1691954/139‑1 (MQ=255)
                                  gAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTa                                                                                         >  2:1647992/1‑139 (MQ=255)
                                  gAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTa                                                                                         >  1:838149/1‑139 (MQ=255)
                                        aTGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGa                                                                                   >  2:322929/1‑139 (MQ=255)
                                         tGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGAGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaa                                                                                  <  1:822803/139‑1 (MQ=255)
                                          gCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaaa                                                                                 >  1:688223/1‑139 (MQ=255)
                                          gCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaaa                                                                                 >  2:380273/1‑139 (MQ=255)
                                                acaacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggcc                                                                           <  2:688223/139‑1 (MQ=255)
                                                 caacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc                                                                                       >  2:9495/1‑126 (MQ=255)
                                                 caacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc                                                                                       <  1:9495/126‑1 (MQ=255)
                                                  aacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc                                                                                       >  1:517263/1‑125 (MQ=255)
                                                  aacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc                                                                                       <  2:517263/125‑1 (MQ=255)
                                                  aacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgg                                                                         <  2:1551669/139‑1 (MQ=255)
                                                    cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                                       >  2:13552/1‑139 (MQ=255)
                                                    cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                                       <  1:34187/139‑1 (MQ=255)
                                                    cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                                       <  1:411004/139‑1 (MQ=255)
                                                    cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                                       >  2:411004/1‑139 (MQ=255)
                                                    cacGAGGATTTATGGCAGACAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACGCATCGCCCTGGGGGGGCACCATTGGCGTCGCCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                                       <  1:709309/139‑1 (MQ=255)
                                                     acGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCAt                                                                                                                         <  2:699272/88‑1 (MQ=255)
                                                     acGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCAt                                                                                                                         >  1:699272/1‑88 (MQ=255)
                                                       gAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCAt                                                                    <  2:838149/139‑1 (MQ=255)
                                                        aGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATc                                                                   >  1:764678/1‑139 (MQ=255)
                                                          gATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCg                                                                 >  2:1694907/1‑139 (MQ=255)
                                                                     gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGc                                                                                                               >  2:841553/1‑82 (MQ=255)
                                                                     gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGc                                                                                                               <  1:841553/82‑1 (MQ=255)
                                                                     gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCAGTATTGTTGGcc                                                      <  1:380273/139‑1 (MQ=255)
                                                                      aTAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggcc                                                                      <  1:674159/122‑1 (MQ=255)
                                                                      aTAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggcc                                                                      >  2:674159/1‑122 (MQ=255)
                                                                       tAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCt                                                     >  2:1147626/1‑138 (MQ=255)
                                                                       tAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCt                                                     <  1:1147626/138‑1 (MQ=255)
                                                                          cAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTatat                                                 <  1:1647992/139‑1 (MQ=255)
                                                                          cAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTatat                                                 <  2:1607519/139‑1 (MQ=255)
                                                                          cAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTggggg                                                                                                                                >  1:1554246/1‑59 (MQ=255)
                                                                          cAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTggggg                                                                                                                                <  2:1554246/59‑1 (MQ=255)
                                                                             aCCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                                       >  2:858373/1‑114 (MQ=255)
                                                                             aCCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                                       <  1:858373/114‑1 (MQ=255)
                                                                                 gagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGc                                                                                                               <  2:1500337/70‑1 (MQ=255)
                                                                                 gagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGc                                                                                                               >  1:1500337/1‑70 (MQ=255)
                                                                                  agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCg                                         >  2:1776931/1‑139 (MQ=255)
                                                                                  agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTggggggg                                                                                                                              >  1:1737182/1‑52 (MQ=255)
                                                                                  agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTggggggg                                                                                                                              <  2:1737182/53‑2 (MQ=255)
                                                                                  agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTggggg                                                                                                                                >  2:1332884/1‑51 (MQ=255)
                                                                                  agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTggggg                                                                                                                                <  1:1332884/51‑1 (MQ=255)
                                                                                    cTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCggg                                       <  2:766810/139‑1 (MQ=255)
                                                                                            tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGc                                                                                                               >  2:1517686/1‑59 (MQ=255)
                                                                                            tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGc                                                                                                               <  1:1517686/59‑1 (MQ=255)
                                                                                            tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGt                               >  2:1274546/1‑139 (MQ=255)
                                                                                            tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGt                               <  1:1694907/139‑1 (MQ=255)
                                                                                               gCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa                                                                                            >  1:370667/1‑75 (MQ=255)
                                                                                               gCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa                                                                                            <  2:370667/75‑1 (MQ=255)
                                                                                               gCTGGAAGCTCGACATATCGAACTCATCGCCCTggggggg                                                                                                                              >  1:1371579/1‑39 (MQ=255)
                                                                                               gCTGGAAGCTCGACATATCGAACTCATCGCCCTggggggg                                                                                                                              <  2:1371579/40‑2 (MQ=255)
                                                                                                     aGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcat                      <  1:1776931/139‑1 (MQ=255)
                                                                                                      gCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcatc                     <  2:1079474/139‑1 (MQ=255)
                                                                                                         cGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCg                                                                                                                    <  1:689525/41‑1 (MQ=255)
                                                                                                         cGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCg                                                                                                                    >  2:689525/1‑41 (MQ=255)
                                                                                                         cGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGc                                                                                                               <  2:1794132/46‑1 (MQ=255)
                                                                                                         cGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGc                                                                                                               >  1:1794132/1‑46 (MQ=255)
                                                                                                         cGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa                                                                                            >  1:1066200/1‑65 (MQ=255)
                                                                                                         cGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa                                                                                            <  2:1066200/65‑1 (MQ=255)
                                                                                                         cGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATg                  >  1:1144972/1‑139 (MQ=255)
                                                                                                           aCATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCg                <  2:1136913/139‑1 (MQ=255)
                                                                                                              tatCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt                                                           >  1:212329/1‑93 (MQ=255)
                                                                                                              tatCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt                                                           <  2:212329/93‑1 (MQ=255)
                                                                                                                 cGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAAt          <  1:446378/139‑1 (MQ=255)
                                                                                                                        aTCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgtt                                                          >  1:831685/1‑84 (MQ=255)
                                                                                                                        aTCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgtt                                                          <  2:831685/84‑1 (MQ=255)
                                                                                                                        aTCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt                                                           >  1:1194701/1‑83 (MQ=255)
                                                                                                                        aTCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt                                                           <  2:1194701/83‑1 (MQ=255)
                                                                                                                         tCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGaaa  >  2:1031471/1‑139 (MQ=255)
                                                                                                                          cGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt                                                           <  1:1307996/81‑1 (MQ=255)
                                                                                                                          cGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt                                                           >  2:1307996/1‑81 (MQ=255)
                                                                                                                                |                                                                                                                                   
CTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAA  >  NC_000913/3980824‑3981082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: