Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 4,474,138 | (A)6→7 | coding (15/594 nt) | bdcR → | transcriptional repressor for divergent bdcA |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,474,132 | 1 | . | A | 98.4% | 206.0 / ‑4.1 | 64 | coding (9/594 nt) | bdcR | transcriptional repressor for divergent bdcA |
Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (36/27); total (37/27) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTT > NC_000913/4474001‑4474261 | aTACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑aa > 1:1105857/1‑134 (MQ=255) aTACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑aa < 2:1105857/134‑1 (MQ=255) tGCCACCGAGGATGAGAACTGTCTTACCTGTACAAGCGCCCATAAGGACTCCTTGATTGATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCATTAAA‑AAAACAATCAcgcg > 2:1731747/1‑139 (MQ=255) gaACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑aa‑aaaaaca < 1:1120751/116‑4 (MQ=255) gaACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑aa‑aaaaaca > 2:1120751/1‑113 (MQ=255) tCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGtt > 1:64807/1‑139 (MQ=255) ttACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTc > 1:1232466/1‑139 (MQ=255) ccTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑aa‑aaaaaca < 1:1245328/105‑4 (MQ=255) ccTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑aa‑aaaaaca > 2:1245328/1‑102 (MQ=255) aaaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGgt > 1:1815803/1‑116 (MQ=255) aaaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGgt < 2:1815803/116‑1 (MQ=255) aaaGCGCCCATAAGGACTACTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg < 2:1232466/139‑1 (MQ=255) tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAAtctc > 1:1015507/1‑87 (MQ=255) tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAAtctc < 2:1015507/87‑1 (MQ=255) cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAAtctc < 2:1228350/85‑1 (MQ=255) cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAg < 2:785855/104‑1 (MQ=255) cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAg > 1:785855/1‑104 (MQ=255) cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg < 2:1320253/120‑1 (MQ=255) cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg > 1:1320253/1‑120 (MQ=255) cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑Tctctctgcggc > 2:1760584/1‑132 (MQ=255) cTTGATTTATCATATAACATGCATTACAAAACTGTTTAAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAAtctc > 1:1228350/1‑85 (MQ=255) attatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑tctc > 1:184646/1‑123 (MQ=255) attatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑tctc < 2:184646/123‑1 (MQ=255) tatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg < 1:1146786/110‑1 (MQ=255) tatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg > 2:1146786/1‑110 (MQ=255) tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCAc > 2:1613768/1‑139 (MQ=255) tAACATACATTCCAAAACTGTCTTCACTTTCTGTAACCAGGCTTTAAAATGCTCACT‑AACAAAACAATCCCGCGCTCCAGCTCGTCCCAAACGTTTCGCTCCTGAGCAGGCAA‑‑TCACCCCACCAAAAGTGCTATTTCAc > 1:679928/1‑139 (MQ=255) aTGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCaaa < 1:1613768/139‑1 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCaaaa > 2:1484485/1‑139 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCaaaa > 1:1688157/1‑139 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTATAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCaaaa > 2:1117909/1‑139 (MQ=255) gCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTCTGCGGCAAAAGTGCTTTTTCACCaaa < 1:1760584/139‑1 (MQ=255) gCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCtttt > 1:436168/1‑129 (MQ=255) gCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCtttt < 2:436168/129‑1 (MQ=255) aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCAcc > 2:309993/1‑132 (MQ=255) aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCAcc < 1:309993/132‑1 (MQ=255) tACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGtt > 1:1704761/1‑139 (MQ=255) aaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGa < 1:1117909/139‑1 (MQ=255) aaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGAt > 1:1375689/1‑139 (MQ=255) cTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAAtctc < 1:518425/54‑1 (MQ=255) cTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAAtctc > 2:518425/1‑54 (MQ=255) cTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGc > 1:1734173/1‑139 (MQ=255) ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGttt < 2:844472/76‑1 (MQ=255) ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGttt > 1:844472/1‑76 (MQ=255) ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGAt > 1:1628616/1‑134 (MQ=255) ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGAt < 2:1628616/134‑1 (MQ=255) ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt > 1:1446722/1‑139 (MQ=255) ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt > 1:1444413/1‑139 (MQ=255) ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑aa < 2:969917/39‑1 (MQ=255) ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑aa > 1:969917/1‑39 (MQ=255) ttttAACTTTCTGTCAACA‑GTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGGTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTc > 2:794107/1‑139 (MQ=255) ttCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTg > 2:936491/1‑139 (MQ=255) cTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGttt > 1:1353224/1‑66 (MQ=255) cTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGttt < 2:1353224/66‑1 (MQ=255) aaCAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGt > 2:1757405/1‑139 (MQ=255) aaCAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGt < 1:1484485/139‑1 (MQ=255) aCAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGtt < 2:1704761/139‑1 (MQ=255) tttGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAt < 2:1734173/139‑1 (MQ=255) tttGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAt < 1:936491/139‑1 (MQ=255) ttGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTa < 1:520996/133‑1 (MQ=255) ttGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTa > 2:520996/1‑133 (MQ=255) ttGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAtt > 1:1817000/1‑139 (MQ=255) ttGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAtt > 2:576929/1‑139 (MQ=255) aTGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCtt < 2:1817000/139‑1 (MQ=255) ggTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGa > 2:755856/1‑41 (MQ=255) ggTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGa < 1:755856/41‑1 (MQ=255) ggTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg < 1:1638030/56‑1 (MQ=255) ggTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg > 2:1638030/1‑56 (MQ=255) ggTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCttttt < 1:241925/86‑1 (MQ=255) ggTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCttttt > 2:241925/1‑86 (MQ=255) ggTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCtt > 2:811878/1‑137 (MQ=255) ggTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCtt < 1:811878/137‑1 (MQ=255) | ATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAA‑‑TCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTT > NC_000913/4474001‑4474261 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |