Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	522,463	T→C	V683A (GTG→GCG)	ybbP →	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	522,463	0	T	C	100.0%	258.7 / NA	72	V683A (GTG→GCG)	ybbP	putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/34);  total (38/34)

AGTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCAC  >  NC_000913/522327‑522595                                                                                                                                         |                                                                                                                                     aGTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGa                                                                                                                                    >  2:390314/1‑139 (MQ=255)          tGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTAc                                                                                                                           <  2:474590/139‑1 (MQ=255)                    gCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGc                                                                                                                 <  1:210271/139‑1 (MQ=255)                        cGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGc                                                                                                                 >  2:907381/1‑135 (MQ=255)                        cGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGc                                                                                                                 <  1:907381/135‑1 (MQ=255)                                   cacaACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTAtgttgct                                                                                                  <  2:628801/139‑1 (MQ=255)                                        cTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTAtgttgctgttgc                                                                                             >  2:1055004/1‑139 (MQ=255)                                        cTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTAtgttgctgttgc                                                                                             >  1:1692330/1‑139 (MQ=255)                                                ccAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCAcag                                                                                     <  1:390314/139‑1 (MQ=255)                                                            cATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGa                                                                         <  1:850347/139‑1 (MQ=255)                                                                      ttAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGGCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgtcagc                                                               >  2:1633656/1‑139 (MQ=255)                                                                             ttGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGtcatca                                                        <  1:374824/139‑1 (MQ=255)                                                                              tGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAAAGCGTCAGCGTCATCAg                                                       >  2:1821545/1‑139 (MQ=255)                                                                                gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCAcaggtg                                                                                  >  1:1570009/1‑110 (MQ=255)                                                                                gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCAcaggtg                                                                                  <  2:1570009/110‑1 (MQ=255)                                                                                gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGa                                                     >  1:516926/1‑139 (MQ=255)                                                                                gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGa                                                     >  1:327922/1‑139 (MQ=255)                                                                                gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGa                                                     >  2:1136216/1‑139 (MQ=255)                                                                                gcgcGATTTTAAAACAGGTAGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGa                                                     >  2:613629/1‑139 (MQ=255)                                                                                   cgATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGAtgg                                                                                                                                 <  2:1122174/60‑1 (MQ=255)                                                                                   cgATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGAtgg                                                                                                                                 >  1:1122174/1‑60 (MQ=255)                                                                                               aGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGgcg                                      <  1:1633656/139‑1 (MQ=255)                                                                                                ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTAc                                                                                                                           <  2:333614/53‑1 (MQ=255)                                                                                                ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTAc                                                                                                                           >  1:333614/1‑53 (MQ=255)                                                                                                ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTAc                                                                                                                           <  2:1338317/53‑1 (MQ=255)                                                                                                ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTAc                                                                                                                           >  1:1338317/1‑53 (MQ=255)                                                                                                ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGc                                                                                                                 <  1:1425875/63‑1 (MQ=255)                                                                                                ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGc                                                                                                                 >  2:1425875/1‑63 (MQ=255)                                                                                                ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCg                                                                                                            >  2:1620742/1‑68 (MQ=255)                                                                                                ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCg                                                                                                            <  1:1620742/68‑1 (MQ=255)                                                                                                ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCAcaggtg                                                                                  >  1:833330/1‑94 (MQ=255)                                                                                                ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCAcaggtg                                                                                  <  2:833330/94‑1 (MQ=255)                                                                                                ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGgcgc                                     >  2:800931/1‑139 (MQ=255)                                                                                                ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGgcgc                                     >  2:1482096/1‑139 (MQ=255)                                                                                                ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGgcgc                                     >  2:851926/1‑139 (MQ=255)                                                                                                ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCACTGGAAGCGa                                                                                                                                    >  2:1111099/1‑44 (MQ=38)                                                                                                ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCACTGGAAGCGa                                                                                                                                    <  1:1111099/44‑1 (MQ=255)                                                                                                 gtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGa                                                                         >  1:1466177/1‑102 (MQ=255)                                                                                                 gtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGa                                                                         <  2:1466177/102‑1 (MQ=255)                                                                                                 gtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgtcagcg                                                              <  2:272829/113‑1 (MQ=255)                                                                                                 gtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgtcagcg                                                              >  1:272829/1‑113 (MQ=255)                                                                                                 gtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTACGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgt                                                                   >  1:713132/1‑108 (MQ=255)                                                                                                 gtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTACGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgt                                                                   <  2:713132/108‑1 (MQ=255)                                                                                                  tcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCa                                                                                                                     >  2:1470380/1‑57 (MQ=255)                                                                                                  tcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCa                                                                                                                     <  1:1470380/57‑1 (MQ=255)                                                                                                     gtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACt                                <  2:1692330/139‑1 (MQ=255)                                                                                                        aGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTa                                                                                                                            >  2:182996/1‑44 (MQ=255)                                                                                                        aGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTa                                                                                                                            <  1:182996/44‑1 (MQ=255)                                                                                                          gTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTg                           <  2:516926/139‑1 (MQ=255)                                                                                                           tGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTggtggt                                            >  1:675565/1‑121 (MQ=255)                                                                                                           tGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTggtggt                                            <  2:675565/121‑1 (MQ=255)                                                                                                           tGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGc                          <  1:1311901/139‑1 (MQ=255)                                                                                                                gAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCt                                                                                                               >  1:249064/1‑49 (MQ=255)                                                                                                                gAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCt                                                                                                               <  2:249064/49‑1 (MQ=255)                                                                                                                gAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGca                                                                                        <  1:579021/72‑1 (MQ=255)                                                                                                                gAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGca                                                                                        >  2:579021/1‑72 (MQ=255)                                                                                                                    aGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATgttgctgttgct                                                                                            <  2:1410132/64‑1 (MQ=255)                                                                                                                    aGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATgttgctgttgct                                                                                            >  1:1410132/1‑64 (MQ=255)                                                                                                                     ggTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTa                                                                                                         >  2:32439/1‑50 (MQ=255)                                                                                                                     ggTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTa                                                                                                         <  1:32439/50‑1 (MQ=255)                                                                                                                     ggTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGaaaaaa                >  1:1823581/1‑139 (MQ=255)                                                                                                                     ggTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGaaaaaa                >  1:1526654/1‑139 (MQ=255)                                                                                                                      gTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGc                                                                                         >  2:950980/1‑65 (MQ=255)                                                                                                                      gTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGc                                                                                         <  1:950980/65‑1 (MQ=255)                                                                                                                          gTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAActgct           <  2:327922/139‑1 (MQ=255)                                                                                                                           tCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCtggtg                                              <  1:1792061/103‑1 (MQ=255)                                                                                                                           tCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCtggtg                                              >  2:1792061/1‑103 (MQ=255)                                                                                                                                cgcTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTggtggt                                            <  2:581215/100‑1 (MQ=255)                                                                                                                                cgcTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTggtggt                                            >  1:581215/1‑100 (MQ=255)                                                                                                                                cgcTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTAc     >  1:1388337/1‑139 (MQ=255)                                                                                                                                   tGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCAc  <  2:1823581/139‑1 (MQ=255)                                                                                                                                   tGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCAc  >  2:1093722/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                     AGTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTACCAC  >  NC_000913/522327‑522595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑