Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 582,152 C→T pseudogene (653/653 nt) ybcY ← pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913582,1520CT100.0% 358.7 / NA 110pseudogene (653/653 nt)ybcYpseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (55/55);  total (55/55)

Only 100 of 110 total aligned reads displayed.

GGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCT  >  NC_000913/582025‑582282
                                                                                                                               |                                                                                                                                  
ggTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTctg                                                                                                                        >  1:1267451/1‑137 (MQ=255)
 gtgtTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTg                                                                                                                       <  2:1401492/139‑1 (MQ=255)
         gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGaa                                                                                                               <  1:1149554/139‑1 (MQ=255)
              gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                          <  1:813127/139‑1 (MQ=255)
              gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                          >  2:1061271/1‑139 (MQ=255)
              gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTCAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                          <  1:1061271/139‑1 (MQ=255)
               tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGaa                                                                                                               <  1:1356532/133‑1 (MQ=255)
               tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGaa                                                                                                               >  2:1356532/1‑133 (MQ=255)
               tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                         >  2:1237920/1‑139 (MQ=255)
                   gATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATtacta                                                                                                     <  2:1707738/139‑1 (MQ=255)
                                cACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGa                                                                                                                >  1:110529/1‑115 (MQ=255)
                                cACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGa                                                                                                                <  2:110529/115‑1 (MQ=255)
                                  ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                          <  2:1443855/119‑1 (MQ=255)
                                  ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                          >  1:1443855/1‑119 (MQ=255)
                                  ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                          >  1:977484/1‑119 (MQ=255)
                                  ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                      >  1:1196840/1‑139 (MQ=255)
                                  ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGACTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                          <  2:977484/119‑1 (MQ=255)
                                   cTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCtt                                                                                     <  2:1622237/139‑1 (MQ=255)
                                     gATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                             <  1:1052627/113‑1 (MQ=255)
                                     gATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                             >  2:1052627/1‑113 (MQ=255)
                                             gTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAcc                                                                                       <  2:991539/127‑1 (MQ=255)
                                                gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                        >  2:606509/1‑139 (MQ=255)
                                                gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                        >  2:112474/1‑139 (MQ=255)
                                                 ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtct                                                                       >  1:1425939/1‑139 (MQ=255)
                                                   tACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCg                                                                     >  1:374692/1‑139 (MQ=255)
                                                    aCGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                   <  2:436074/108‑1 (MQ=255)
                                                    aCGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                   >  1:436074/1‑108 (MQ=255)
                                                     cGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTT‑CTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                  <  2:1267451/139‑1 (MQ=255)
                                                      gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                             <  2:100858/128‑1 (MQ=255)
                                                      gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                             >  1:100858/1‑128 (MQ=255)
                                                       aaCCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCCTGAACCTTGGTTTTAACATTCTCGaaa                                                                  <  1:1520221/138‑1 (MQ=255)
                                                          ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                              >  2:1803381/1‑139 (MQ=255)
                                                          ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                              <  2:1320430/139‑1 (MQ=255)
                                                          ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                              <  1:40008/139‑1 (MQ=255)
                                                          ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                              >  2:1709978/1‑139 (MQ=255)
                                                           cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGa                                                                                                                      >  1:89924/1‑82 (MQ=255)
                                                           cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGa                                                                                                                      <  2:89924/82‑1 (MQ=255)
                                                            tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                            <  1:1709978/139‑1 (MQ=255)
                                                              tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                          <  1:1803381/139‑1 (MQ=255)
                                                               cAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                         <  1:627274/91‑1 (MQ=255)
                                                                    aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                    <  1:659358/139‑1 (MQ=255)
                                                                            ttGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAcc                                                                                              >  1:322988/1‑89 (MQ=255)
                                                                            ttGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAcc                                                                                              <  2:322988/89‑1 (MQ=255)
                                                                              gggATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaa                                                                   <  2:1724041/114‑1 (MQ=255)
                                                                              gggATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaa                                                                   >  1:1724041/1‑114 (MQ=255)
                                                                              gggATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                    >  2:31545/1‑113 (MQ=255)
                                                                              gggATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                              >  2:721476/1‑119 (MQ=255)
                                                                              gggATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                              <  1:721476/119‑1 (MQ=255)
                                                                                gATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAcc                                                                                       <  2:495886/92‑1 (MQ=255)
                                                                                gATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAcc                                                                                       >  1:495886/1‑92 (MQ=255)
                                                                                    aTGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttct                                    >  2:1610781/1‑139 (MQ=255)
                                                                                      gCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                             <  1:1702014/96‑1 (MQ=255)
                                                                                      gCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                             >  2:1702014/1‑96 (MQ=255)
                                                                                          cagcaCGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATta                                                                                                        >  2:294917/1‑65 (MQ=255)
                                                                                          cagcaCGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATta                                                                                                        <  1:294917/65‑1 (MQ=255)
                                                                                          cagcaCGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                             >  1:1835453/1‑92 (MQ=255)
                                                                                          cagcaCGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                             <  2:1835453/92‑1 (MQ=255)
                                                                                          cagcaCGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTaa                                           <  2:690668/126‑1 (MQ=255)
                                                                                          cagcaCGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTaa                                           >  1:690668/1‑126 (MQ=255)
                                                                                          cagcaCGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCgg                                >  2:1259300/1‑137 (MQ=255)
                                                                                          cagcaCGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCgg                                <  1:1259300/137‑1 (MQ=255)
                                                                                               cGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGtttt                                                                               <  2:1097704/85‑1 (MQ=255)
                                                                                               cGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGtttt                                                                               >  1:1097704/1‑85 (MQ=255)
                                                                                               cGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                          <  1:1561908/106‑1 (MQ=255)
                                                                                               cGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                          >  2:1561908/1‑106 (MQ=255)
                                                                                               cGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCttt                         >  2:1166669/1‑139 (MQ=255)
                                                                                               cGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCttt                         <  1:1237920/139‑1 (MQ=255)
                                                                                                gTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                    <  2:935361/95‑1 (MQ=255)
                                                                                                gTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                    >  1:935361/1‑95 (MQ=255)
                                                                                                  cTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                            >  1:411112/1‑101 (MQ=255)
                                                                                                  cTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                            <  2:411112/101‑1 (MQ=255)
                                                                                                  cTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                              >  2:62294/1‑99 (MQ=255)
                                                                                                  cTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                              <  1:62294/99‑1 (MQ=255)
                                                                                                       gCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGaa                                                                                                               <  2:72015/45‑1 (MQ=37)
                                                                                                       gCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGaa                                                                                                               >  1:72015/1‑45 (MQ=38)
                                                                                                         aaGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                             >  1:760368/1‑77 (MQ=255)
                                                                                                         aaGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                             <  2:760368/77‑1 (MQ=255)
                                                                                                          aGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGa                               <  1:771401/122‑1 (MQ=255)
                                                                                                          aGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGa                               >  2:771401/1‑122 (MQ=255)
                                                                                                          aGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaa              >  2:812579/1‑139 (MQ=255)
                                                                                                          aGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaa              >  1:5441/1‑139 (MQ=255)
                                                                                                           gAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                    >  2:1425112/1‑84 (MQ=255)
                                                                                                           gAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                    <  1:1425112/84‑1 (MQ=255)
                                                                                                            aaCATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCg                                                                                                                  >  1:1437226/1‑37 (MQ=25)
                                                                                                            aaCATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCg                                                                                                                  <  2:1437226/37‑1 (MQ=25)
                                                                                                            aaCATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                             >  1:1404313/1‑42 (MQ=37)
                                                                                                            aaCATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                             <  2:1404313/42‑1 (MQ=37)
                                                                                                            aaCATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGaa                                                                                                               <  1:239768/40‑1 (MQ=25)
                                                                                                                tgctgcGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGc        >  2:275495/1‑139 (MQ=255)
                                                                                                                  ctgcGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCt      >  1:1794599/1‑139 (MQ=255)
                                                                                                                    gcGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt    <  1:275495/139‑1 (MQ=255)
                                                                                                                    gcGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt    <  2:1425939/139‑1 (MQ=255)
                                                                                                                     gctgtttaTGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGCTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt    <  1:1122014/132‑1 (MQ=255)
                                                                                                                     cGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                  <  1:996362/76‑1 (MQ=255)
                                                                                                                     cGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                          <  2:1726368/84‑1 (MQ=255)
                                                                                                                     cGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                          >  1:1726368/1‑84 (MQ=255)
                                                                                                                     cGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGAt          <  2:32427/132‑1 (MQ=255)
                                                                                                                     cGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGAt          >  1:32427/1‑132 (MQ=255)
                                                                                                                       ggttgATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                    >  1:1105168/5‑73 (MQ=255)
                                                                                                                       ggttgATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                    <  2:1105168/69‑1 (MQ=255)
                                                                                                                               |                                                                                                                                  
GGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCT  >  NC_000913/582025‑582282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑