Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 586,280 T→C N210S (AAC→AGC)  envY ← porin thermoregulatory transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913586,2800TC100.0% 382.6 / NA 106N210S (AAC→AGC) envYporin thermoregulatory transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (51/55);  total (51/55)

Only 100 of 106 total aligned reads displayed.

TTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTGGGGCTTAAACATAGCGAACTGGCGACAATTCGCA  >  NC_000913/586146‑586415
                                                                                                                                      |                                                                                                                                       
ttCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATc                                                                                                                                     >  1:1682832/1‑139 (MQ=255)
 tCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATcc                                                                                                                                    <  1:443704/139‑1 (MQ=255)
  caccacATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCa                                                                                                                                   <  2:26929/139‑1 (MQ=255)
  caccacATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCa                                                                                                                                   <  1:627467/139‑1 (MQ=255)
     cacaTCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCa                                                                                                                                <  2:686316/139‑1 (MQ=255)
       caTCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTa                                                                                                                                       >  2:1346014/1‑130 (MQ=255)
       caTCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTa                                                                                                                                       <  1:1346014/130‑1 (MQ=255)
               tttGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCAATCAATAACATCTg                                                                                                                      >  1:1502674/1‑139 (MQ=255)
                 tGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGGTAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGGGTGATATTTTTGCTTGCCAGCAATAACATCTGTa                                                                                                                    <  1:1803060/139‑1 (MQ=255)
                 tGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCa                                                                                                                                   >  1:501301/1‑124 (MQ=255)
                 tGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCa                                                                                                                                   <  2:501301/124‑1 (MQ=255)
                    cGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACgg                                                                                                                 >  2:323539/1‑139 (MQ=255)
                      cTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCg                                                                                                               >  2:603053/1‑139 (MQ=255)
                       tGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGt                                                                                                              >  2:1028885/1‑139 (MQ=255)
                        gTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTa                                                                                                             <  2:550352/139‑1 (MQ=255)
                                 gaTAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATcc                                                                                                                                    <  2:400922/107‑1 (MQ=255)
                                 gaTAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATcc                                                                                                                                    >  1:400922/1‑107 (MQ=255)
                                 gaTAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATcc                                                                                                                                    >  1:589688/1‑107 (MQ=255)
                                 gaTAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATcc                                                                                                                                    <  2:589688/107‑1 (MQ=255)
                                 gaTAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCa                                                                                                                                   >  2:425616/1‑108 (MQ=255)
                                 gaTAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCa                                                                                                                                   <  1:425616/108‑1 (MQ=255)
                                  aTAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGa                                                                                                   <  2:1502674/139‑1 (MQ=255)
                                    aaTTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGaca                                                                                                 <  1:1680488/139‑1 (MQ=255)
                                              tgtCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGGCGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTa                                                                                                                                       <  2:1414176/91‑1 (MQ=255)
                                              tgtCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTTCGCCACCTGAGTGATATTTTTGCTa                                                                                                                                       >  1:1414176/1‑91 (MQ=255)
                                              tgtCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATc                                                                                                                                     <  1:576028/93‑1 (MQ=255)
                                              tgtCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATc                                                                                                                                     >  2:576028/1‑93 (MQ=255)
                                              tgtCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATaa                                                                                                                            <  1:766738/102‑1 (MQ=255)
                                              tgtCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATaa                                                                                                                            >  2:766738/1‑102 (MQ=255)
                                                tCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTa                                                                                                                    >  2:149553/1‑108 (MQ=255)
                                                tCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTa                                                                                                                    >  2:1329015/1‑108 (MQ=255)
                                                tCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTa                                                                                                                    <  1:1329015/108‑1 (MQ=255)
                                                tCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTa                                                                                                                    <  1:149553/108‑1 (MQ=255)
                                                tCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAAt                                                                                     <  1:323539/139‑1 (MQ=255)
                                                 cAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGaca                                                                                                 <  1:1821896/126‑1 (MQ=255)
                                                 cAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGaca                                                                                                 >  2:1821896/1‑126 (MQ=255)
                                                     ccGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGa                                                                                                   >  1:1352965/1‑120 (MQ=255)
                                                     ccGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGa                                                                                                   <  2:1352965/120‑1 (MQ=255)
                                                      cGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCt                                                                               <  2:1682832/139‑1 (MQ=255)
                                                       gTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCta                                                                              <  1:1028885/139‑1 (MQ=255)
                                                        tAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCa                                                                                                                                   <  1:558367/85‑1 (MQ=255)
                                                        tAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCa                                                                                                                                   >  2:558367/1‑85 (MQ=255)
                                                        tAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAAt                                                                                                                              >  1:612852/1‑90 (MQ=255)
                                                        tAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAAt                                                                                                                              <  2:612852/90‑1 (MQ=255)
                                                        tAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTa                                                                                                                    <  1:858863/100‑1 (MQ=255)
                                                        tAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTa                                                                                                                    >  2:858863/1‑100 (MQ=255)
                                                            aCGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCt                                                                         >  1:1625368/1‑139 (MQ=255)
                                                              gCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAAt                                                                                                                              <  1:131647/84‑1 (MQ=255)
                                                              gCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAAt                                                                                                                              >  2:131647/1‑84 (MQ=255)
                                                                            gaTAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATCTTTTTGCTATCCCTCAATAACATCTGTATGGCGTAACGCATACGACACTCTGTGACAAt                                                                                     >  2:1003489/1‑111 (MQ=255)
                                                                            gaTAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAAt                                                                                     <  1:1003489/111‑1 (MQ=255)
                                                                                     cgacgTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACAct                                                                                               <  2:477575/92‑1 (MQ=255)
                                                                                      gacgTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTAt                                                                                                                                      <  1:247290/52‑1 (MQ=255)
                                                                                      gacgTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTAt                                                                                                                                      >  2:247290/1‑52 (MQ=255)
                                                                                          tgctgcTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGc                                                                          >  1:330498/1‑108 (MQ=255)
                                                                                          tgctgcTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGc                                                                          <  2:330498/108‑1 (MQ=255)
                                                                                           gctgctATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAAt                                                                                     <  2:1297018/96‑1 (MQ=255)
                                                                                           gctgctATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGc                                          >  2:1442347/1‑139 (MQ=255)
                                                                                           gctgctATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCAATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAAt                                                                                     >  1:1297018/1‑96 (MQ=255)
                                                                                             tgctATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCg                                                               >  2:874190/1‑116 (MQ=255)
                                                                                             tgctATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCg                                                               <  1:874190/116‑1 (MQ=255)
                                                                                                tataGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCt                                     <  1:1442347/139‑1 (MQ=255)
                                                                                                tataGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCt                                     <  2:1625368/139‑1 (MQ=255)
                                                                                                 ataGCCACATAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTg                                    >  2:1374598/1‑139 (MQ=255)
                                                                                                       acaTAATTGCGCCACCTGAGTGATATTTTTGCTAt                                                                                                                                      <  1:1612051/35‑1 (MQ=255)
                                                                                                       acaTAATTGCGCCACCTGAGTGATATTTTTGCTAt                                                                                                                                      >  2:1612051/1‑35 (MQ=255)
                                                                                                         aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAAt                                                                                                                              >  1:739450/1‑41 (MQ=255)
                                                                                                         aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAAt                                                                                                                              <  2:739450/41‑1 (MQ=255)
                                                                                                         aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTa                                                                                                             >  1:871911/1‑58 (MQ=255)
                                                                                                         aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTa                                                                                                             <  2:871911/58‑1 (MQ=255)
                                                                                                         aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAAc                                                                                                           <  1:132847/60‑1 (MQ=255)
                                                                                                         aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAAc                                                                                                           >  2:1834867/1‑60 (MQ=255)
                                                                                                         aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAAc                                                                                                           <  1:1834867/60‑1 (MQ=255)
                                                                                                         aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAAc                                                                                                           >  2:132847/1‑60 (MQ=255)
                                                                                                         aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGa                                                                                                   <  1:1497678/68‑1 (MQ=255)
                                                                                                         aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGa                                                                                                   >  2:1497678/1‑68 (MQ=255)
                                                                                                         aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCtgtg                                                                                          >  2:915800/1‑77 (MQ=255)
                                                                                                         aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAAt                                                                                     >  1:1680706/1‑82 (MQ=255)
                                                                                                         aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAAt                                                                                     <  2:1680706/82‑1 (MQ=255)
                                                                                                         aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAg                                           >  1:1657670/1‑124 (MQ=255)
                                                                                                         aTAATTGCGCCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAg                                           <  2:1657670/124‑1 (MQ=255)
                                                                                                               gcgcCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGaca                                                                                                 >  1:1567686/1‑64 (MQ=255)
                                                                                                                cgcCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGc                                          >  2:731137/1‑118 (MQ=255)
                                                                                                                cgcCACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGc                                          <  1:731137/118‑1 (MQ=255)
                                                                                                                  ccACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGa                                                                                         >  2:1516949/1‑69 (MQ=255)
                                                                                                                   cACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAAt                                                                                     <  2:1421766/72‑1 (MQ=255)
                                                                                                                   cACCTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAAt                                                                                     >  1:1421766/1‑72 (MQ=255)
                                                                                                                     ccTGAGTGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTGGGGCTTAAACATAGCGAACt                >  1:902664/1‑139 (MQ=255)
                                                                                                                           tGATATTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTGGGGCTTAAACATAGCGAACTGGCGAc          >  1:1093577/1‑139 (MQ=255)
                                                                                                                             atatTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTGGGGCTTAAACATAGCGAACTGGCGACaa        >  2:415841/1‑139 (MQ=255)
                                                                                                                             atatTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTGGGGCTTAAACATAGCGAACTGGCGACaa        >  1:77506/1‑139 (MQ=255)
                                                                                                                               atTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCg                                                               <  1:1479465/82‑1 (MQ=255)
                                                                                                                               atTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCg                                                               >  2:1479465/1‑82 (MQ=255)
                                                                                                                               atTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTgggg                                 >  2:81286/1‑112 (MQ=255)
                                                                                                                               atTTTTGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTgggg                                 <  1:81286/112‑1 (MQ=255)
                                                                                                                                tttttGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTGGGGCTTAAACATAGCGAACTGGCGACAATTc     >  1:1193475/1‑139 (MQ=255)
                                                                                                                                 ttttGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTGGGGCTTAAACATAGCGAACTGGCGACAATTCg    <  1:603053/139‑1 (MQ=255)
                                                                                                                                   ttGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTGGGGCTTAAACATAGCGAACTGGCGACAATTCGCa  <  1:415841/139‑1 (MQ=255)
                                                                                                                                   ttGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTGGGGCTTAAACATAGCGAACTGGCGACAATTCGCa  <  2:77506/139‑1 (MQ=255)
                                                                                                                                   ttGCTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTGGGGCTTAAACATAGCGAACTGGCGACAATTCGCa  >  2:213163/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                       
TTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGTGACAATCTGGCTATAGCTGGTATTTTCGTTTTTTAATTTCTTTTTGAGCAGGCTGGGGCTTAAACATAGCGAACTGGCGACAATTCGCA  >  NC_000913/586146‑586415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: