Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	649,372	A→G	F67S (TTC→TCC)	citF ←	citrate lyase, citrate‑ACP transferase (alpha) subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	649,372	0	A	G	100.0%	202.6 / NA	57	F67S (TTC→TCC)	citF	citrate lyase, citrate‑ACP transferase (alpha) subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/27);  total (30/27)

GTGTTCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAA  >  NC_000913/649242‑649490                                                                                                                                   |                                                                                                                       gtgtTCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAAcc                                                                                                                >  1:359537/1‑139 (MQ=255)    ttCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTc                                                                                                             >  1:1258198/1‑139 (MQ=255)     tCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCa                                                                                                            >  2:1794892/1‑139 (MQ=255)       tACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATg                                                                                                          <  1:577735/139‑1 (MQ=255)         ccAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGcc                                                                                                        >  2:101944/1‑139 (MQ=255)         ccAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGcc                                                                                                        >  1:1323039/1‑139 (MQ=255)         ccAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGcc                                                                                                        >  1:1373874/1‑139 (MQ=255)          cAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg                                                                                                       <  2:1548098/139‑1 (MQ=255)          cAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg                                                                                                       <  2:359537/139‑1 (MQ=255)          cAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg                                                                                                       <  1:246663/139‑1 (MQ=255)                 gcATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTa                                                                                                <  1:101944/139‑1 (MQ=255)                  cATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTaa                                                                                               >  1:1285207/1‑139 (MQ=255)                          tCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg                                                                                                       <  2:299929/123‑1 (MQ=255)                          tCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg                                                                                                       >  1:299929/1‑123 (MQ=255)                               cAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGcgacga                                                                                  <  2:1323039/139‑1 (MQ=255)                                aGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGcga                                                                                     >  2:364048/1‑135 (MQ=255)                                aGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGcga                                                                                     <  1:364048/135‑1 (MQ=255)                                aGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGaa                                                                                 >  2:567224/1‑139 (MQ=255)                                aGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGaa                                                                                 <  2:1373874/139‑1 (MQ=255)                                 gggAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATTTTCGCGTTGACGTCCATCACCATATTGAGGGTCGGGTACCGGCCCCGGAAAGGGTGAGGGGGGGAAACGGTCATGCCGTCCTGTAAACCAGAGGGACGAAt                                                                                >  2:526649/1‑139 (MQ=255)                                 gggAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg                                                                                                       <  2:1481108/116‑1 (MQ=255)                                 gggAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg                                                                                                       >  1:1481108/1‑116 (MQ=255)                                     gCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGct                                                                            <  2:39950/139‑1 (MQ=255)                                            gCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCa                                                                     >  2:802391/1‑139 (MQ=255)                                                    cAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCGGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGgcg                                                             <  1:526649/139‑1 (MQ=255)                                                    cAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGgcg                                                             <  2:1285207/139‑1 (MQ=255)                                                      ggTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGca                                                           <  2:1258198/139‑1 (MQ=255)                                                      ggTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGca                                                           <  2:945955/139‑1 (MQ=255)                                                      ggTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGca                                                           <  1:567224/139‑1 (MQ=255)                                                                    aTCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGca                                                           <  1:1485480/125‑1 (MQ=255)                                                                    aTCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGca                                                           >  2:1485480/1‑125 (MQ=255)                                                                     tCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATcgcg                                            <  2:643908/139‑1 (MQ=255)                                                                     tCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATcgcg                                            >  2:464975/1‑139 (MQ=255)                                                                              tGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTc                                   <  1:802391/139‑1 (MQ=255)                                                                               gACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCa                                  >  1:435961/1‑139 (MQ=255)                                                                                             tatTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTg                    >  2:121415/1‑139 (MQ=255)                                                                                             tatTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTg                    >  1:1654349/1‑139 (MQ=255)                                                                                                tGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCtt                 >  1:330046/1‑139 (MQ=255)                                                                                                  aCGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTg               >  1:293946/1‑139 (MQ=255)                                                                                                  aCGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGCTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTg               >  1:1519729/1‑139 (MQ=255)                                                                                                     gtcaggtcaCCGCCACGGAAAGCGTGATgggagga                                                                                                                    >  2:669384/1‑35 (MQ=255)                                                                                                     gtcaggtcaCCGCCACGGAAAGCGTGATgggagga                                                                                                                    <  1:669384/35‑1 (MQ=255)                                                                                                     gtcaggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg                                                                                                       >  1:1444299/1‑48 (MQ=255)                                                                                                     gtcaggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg                                                                                                       <  2:1444299/48‑1 (MQ=255)                                                                                                     gtcaggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGt                       >  2:1801792/1‑128 (MQ=255)                                                                                                     gtcaggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGt                       <  1:1801792/128‑1 (MQ=255)                                                                                                         ggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg                                                                                                       >  2:759580/1‑44 (MQ=255)                                                                                                         ggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg                                                                                                       <  1:759580/44‑1 (MQ=255)                                                                                                          gtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTc       >  2:1605958/1‑139 (MQ=255)                                                                                                               cGCCACGGATAGCGTGATGGGAGCATACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCTCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGaa  <  1:464975/139‑1 (MQ=255)                                                                                                               cGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAAc                                                                                             <  2:1766193/48‑1 (MQ=255)                                                                                                               cGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAAc                                                                                             >  1:1766193/1‑48 (MQ=255)                                                                                                                    cGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGTTTCTTCCAGGTTGGCGca                                                           <  2:713568/77‑1 (MQ=255)                                                                                                                    cGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGTTTCTTCCAGGTTGGCGca                                                           >  1:713568/1‑77 (MQ=255)                                                                                                                    cGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATcgcg                                            >  2:1380719/1‑92 (MQ=255)                                                                                                                    cGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATcgcg                                            <  1:1380719/92‑1 (MQ=255)                                                                                                                           cGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGc             <  2:427637/116‑1 (MQ=255)                                                                                                                           cGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGc             >  1:427637/1‑116 (MQ=255)                                                                                                                            gTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATcgcgcg                                          >  2:548042/1‑86 (MQ=255)                                                                                                                            gTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATcgcgcg                                          <  1:548042/86‑1 (MQ=255)                                                                                                                                   |                                                                                                                       GTGTTCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAA  >  NC_000913/649242‑649490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑