Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	649,648	A→G	G281G (GGT→GGC)	citE ←	citrate lyase, citryl‑ACP lyase (beta) subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	649,648	0	A	G	100.0%	193.4 / NA	54	G281G (GGT→GGC)	citE	citrate lyase, citryl‑ACP lyase (beta) subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/27);  total (27/27)

ACGATTCCAGGCCGCTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAAT  >  NC_000913/649515‑649782                                                                                                                                      |                                                                                                                                       acgaTTCCAGGCCGCTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTc                                                                                                                                   <  2:435961/139‑1 (MQ=255)               cTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCtt                                                                                                                          <  2:859913/134‑1 (MQ=255)               cTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCtt                                                                                                                          >  1:859913/1‑134 (MQ=255)               cTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGt                                                                                                                     >  1:1207146/1‑139 (MQ=255)                tACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGtt                                                                                                                    >  1:1583147/1‑139 (MQ=255)                        cTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGaaa                                                                                                            <  1:1718499/139‑1 (MQ=255)                         ttGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAAc                                                                                                           >  2:1158406/1‑139 (MQ=255)                          tGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAAcc                                                                                                          <  2:1207146/139‑1 (MQ=255)                            tCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCAc                                                                                                        >  1:1571185/1‑139 (MQ=255)                                              tttCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAgc                                                                                      >  2:983374/1‑139 (MQ=255)                                               ttCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCATGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAgcg                                                                                     <  2:1005313/139‑1 (MQ=255)                                                     gTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCt                                                                               <  1:983374/139‑1 (MQ=255)                                                                   cTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAg                                                                                                  >  1:1784677/1‑106 (MQ=255)                                                                   cTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAg                                                                                                  <  2:1784677/106‑1 (MQ=255)                                                                            cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCa                                                                            <  1:993850/119‑1 (MQ=255)                                                                            cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCa                                                                            >  2:993850/1‑119 (MQ=255)                                                                            cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACgcggcg                                                        >  1:225218/1‑139 (MQ=255)                                                                            cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACgcggcg                                                        >  2:1573750/1‑139 (MQ=255)                                                                            cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACgcggcg                                                        >  2:1502163/1‑139 (MQ=255)                                                                            cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACgcggcg                                                        >  1:18755/1‑139 (MQ=255)                                                                            cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACgcggcg                                                        >  2:1480418/1‑139 (MQ=255)                                                                             gcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGcggcgg                                                       <  1:1480418/139‑1 (MQ=255)                                                                                 aTGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTcca                                                                                                                                 <  1:1438085/61‑1 (MQ=255)                                                                                 aTGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTcca                                                                                                                                 >  2:1438085/1‑61 (MQ=255)                                                                                 aTGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGt                                                                                                                     >  2:898822/1‑73 (MQ=255)                                                                                 aTGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGt                                                                                                                     <  1:898822/73‑1 (MQ=255)                                                                                 aTGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGt                                                   >  2:1684725/1‑139 (MQ=255)                                                                                  tGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTg                                                  <  2:18755/139‑1 (MQ=255)                                                                                    ccGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGc                                                                       <  2:1350012/116‑1 (MQ=255)                                                                                    ccGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGc                                                                       >  1:1350012/1‑116 (MQ=255)                                                                                     cGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATc                                               <  2:1571185/139‑1 (MQ=255)                                                                                               tGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCg                                                                                                                      <  2:635244/58‑1 (MQ=255)                                                                                               tGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCg                                                                                                                      >  1:635244/1‑58 (MQ=255)                                                                                                      gagaGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCg                              <  2:1228697/139‑1 (MQ=255)                                                                                                      gagaGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCg                              <  2:225218/139‑1 (MQ=255)                                                                                                         agCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTg                                                                                                                         >  2:1092290/1‑45 (MQ=255)                                                                                                         agCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTg                                                                                                                         <  1:1092290/45‑1 (MQ=255)                                                                                                              cAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTag                      <  1:1502163/139‑1 (MQ=255)                                                                                                               aGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTaga                     <  2:1583147/139‑1 (MQ=255)                                                                                                                gACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCa                                                                            >  2:1429700/1‑83 (MQ=255)                                                                                                                gACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCa                                                                            <  1:1429700/83‑1 (MQ=255)                                                                                                                gACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGc                                                                          >  2:165845/1‑85 (MQ=255)                                                                                                                gACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGc                                                                          <  1:165845/85‑1 (MQ=255)                                                                                                                gACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACgcgg                                                          <  2:261423/101‑1 (MQ=255)                                                                                                                gACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACgcgg                                                          >  1:261423/1‑101 (MQ=255)                                                                                                                gACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCAct                                           <  1:1547233/116‑1 (MQ=255)                                                                                                                gACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCAct                                           >  2:1547233/1‑116 (MQ=255)                                                                                                                gACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTagag                    >  1:274841/1‑139 (MQ=255)                                                                                                                 aCGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAgg                   <  1:1684725/139‑1 (MQ=255)                                                                                                                     gcgcGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTtgt               >  1:1769591/1‑139 (MQ=255)                                                                                                                        cgatcgatAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTgca            >  2:1384559/1‑139 (MQ=255)                                                                                                                             gatAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGa       <  2:493659/139‑1 (MQ=255)                                                                                                                               tAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATc     >  2:1348624/1‑139 (MQ=255)                                                                                                                                  ccGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAAt  <  1:1348624/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       ACGATTCCAGGCCGCTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAAT  >  NC_000913/649515‑649782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑