Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	892,869	G→A	*301* (TAG→TAA)	rimK →	ribosomal protein S6 modification protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	892,869	0	G	A	100.0%	240.7 / NA	74	*301* (TAG→TAA)	rimK	ribosomal protein S6 modification protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/37);  total (37/37)

GGTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAGTCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCGTCAAT  >  NC_000913/892737‑892999                                                                                                                                     |                                                                                                                                   ggTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCaa                                                                                                                              <  1:1399052/139‑1 (MQ=255)  gTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                             <  2:284109/139‑1 (MQ=255)     aTGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCaa                                                                                                                              >  2:635435/1‑135 (MQ=255)     aTGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCaa                                                                                                                              <  1:635435/135‑1 (MQ=255)          ggTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCaa                                                                                                                              >  1:10517/1‑130 (MQ=255)          ggTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCaa                                                                                                                              <  2:10517/130‑1 (MQ=255)           gTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                           >  1:746587/1‑132 (MQ=255)           gTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                           <  2:746587/132‑1 (MQ=255)              aaTGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGAt                                                                                                                 >  1:185820/1‑139 (MQ=255)               aTGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGCTCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                             >  2:580467/1‑126 (MQ=255)               aTGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                             <  1:580467/126‑1 (MQ=255)                     cGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGtt                                                                                                          >  1:802455/1‑139 (MQ=255)                          gggCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCaa                                                                                                                              >  2:1682995/1‑114 (MQ=255)                          gggCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCaa                                                                                                                              <  1:1682995/114‑1 (MQ=255)                            gCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTg                                                                                                   >  1:1397820/1‑139 (MQ=255)                             cTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTgc                                                                                                  <  2:852220/139‑1 (MQ=255)                                  aggaaTAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCttt                                                                                             <  2:1397820/139‑1 (MQ=255)                                               accaccGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                           <  1:1531468/96‑1 (MQ=255)                                               accaccGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                           >  2:1531468/1‑96 (MQ=255)                                                   ccGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCg                                                                            <  2:185820/139‑1 (MQ=255)                                                              aTCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                             <  1:234451/79‑1 (MQ=255)                                                              aTCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                             >  2:234451/1‑79 (MQ=255)                                                                cgcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTa                                                                                                                      >  2:642542/1‑84 (MQ=255)                                                                cgcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTa                                                                                                                      <  1:642542/84‑1 (MQ=255)                                                                 gcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGc                                                                                                                                <  2:1141048/73‑1 (MQ=255)                                                                 gcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGc                                                                                                                                >  1:1141048/1‑73 (MQ=255)                                                                 gcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                             <  2:710635/76‑1 (MQ=255)                                                                 gcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                             >  1:710635/1‑76 (MQ=255)                                                                 gcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCtttt                                                                                            >  1:6584/1‑109 (MQ=255)                                                                 gcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCtttt                                                                                            <  2:6584/109‑1 (MQ=255)                                                                     gTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCACTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAagag                                                          >  1:210371/1‑139 (MQ=255)                                                                      tAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCACTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAgg                                                         <  2:210371/139‑1 (MQ=255)                                                                             aTCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                             >  2:1780256/1‑64 (MQ=255)                                                                             aTCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                             <  1:1780256/64‑1 (MQ=255)                                                                               ccGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTa                                                                   >  1:658717/1‑120 (MQ=255)                                                                               ccGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTa                                                                   <  2:658717/120‑1 (MQ=255)                                                                                   tGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATc                                                                                                                <  2:1602207/71‑1 (MQ=255)                                                                                   tGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATc                                                                                                                >  1:1602207/1‑71 (MQ=255)                                                                                          aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGAc                                        <  1:1254220/136‑1 (MQ=255)                                                                                          aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGAc                                        >  2:1254220/1‑136 (MQ=255)                                                                                          aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATc                                     >  2:1412701/1‑139 (MQ=255)                                                                                             gCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCt                                  >  2:853019/1‑139 (MQ=255)                                                                                               cACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCa                                                                                                               <  2:117728/60‑1 (MQ=255)                                                                                               cACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCa                                                                                                               >  1:117728/1‑60 (MQ=255)                                                                                                 cGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTAc                                                   <  2:304869/118‑1 (MQ=255)                                                                                                 cGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTAc                                                   >  1:304869/1‑118 (MQ=255)                                                                                                    tACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGt                               >  2:475074/1‑135 (MQ=255)                                                                                                    tACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGt                               <  1:475074/135‑1 (MQ=255)                                                                                                     aCGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTc                          <  2:802455/139‑1 (MQ=255)                                                                                                         cAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATc                                                                                                                >  1:509929/1‑49 (MQ=255)                                                                                                         cAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATc                                                                                                                <  2:509929/49‑1 (MQ=255)                                                                                                            aaTATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                           >  1:393149/1‑35 (MQ=38)                                                                                                            aaTATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                           <  2:393149/35‑1 (MQ=38)                                                                                                            aaTATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATc                                                                                                                <  2:1645113/46‑1 (MQ=255)                                                                                                            aaTATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATc                                                                                                                >  1:1645113/1‑46 (MQ=255)                                                                                                                ttGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCg                                                                                        <  2:1528041/66‑1 (MQ=255)                                                                                                                ttGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCg                                                                                        >  1:1528041/1‑66 (MQ=255)                                                                                                                   ccTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGt                                                                                       >  1:688850/1‑64 (MQ=255)                                                                                                                   ccTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGt                                                                                       <  2:688850/64‑1 (MQ=255)                                                                                                                   ccTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTa                                                                                      <  2:133278/65‑1 (MQ=255)                                                                                                                   ccTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTa                                                                                      >  1:133278/1‑65 (MQ=255)                                                                                                                      gAAAACGGGTGGTTAATCGCAATCACATTACTGATc                                                                                                                >  1:1083718/1‑36 (MQ=255)                                                                                                                      gAAAACGGGTGGTTAATCGCAATCACATTACTGATc                                                                                                                <  2:1083718/36‑1 (MQ=255)                                                                                                                      gAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAgg                                                         <  2:597684/91‑1 (MQ=255)                                                                                                                      gAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAgg                                                         >  1:597684/1‑91 (MQ=255)                                                                                                                      gAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTg         >  1:128212/1‑139 (MQ=255)                                                                                                                       aaaaCGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTAc                                                   <  2:341291/96‑1 (MQ=255)                                                                                                                       aaaaCGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTAc                                                   >  1:341291/1‑96 (MQ=255)                                                                                                                        aaaCGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCg       >  2:1572093/1‑139 (MQ=255)                                                                                                                             ggtggtTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACa                                                  <  1:970772/91‑1 (MQ=255)                                                                                                                             ggtggtTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACa                                                  >  2:970772/1‑91 (MQ=255)                                                                                                                             ggtggtTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCGTCAAt  <  1:1572093/139‑1 (MQ=255)                                                                                                                                 gtTAATCGCAATCACATTACTGATCATGGTTTTGCCTg                                                                                                   <  2:331955/38‑1 (MQ=255)                                                                                                                                 gtTAATCGCAATCACATTACTGATCATGGTTTTGCCTg                                                                                                   >  1:331955/1‑38 (MQ=38)                                                                                                                                     |                                                                                                                                   GGTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAGTCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCGTCAAT  >  NC_000913/892737‑892999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑