Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	915,730	T→C	K106K (AAA→AAG)	aqpZ ←	aquaporin Z

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	915,730	0	T	C	100.0%	225.9 / NA	62	K106K (AAA→AAG)	aqpZ	aquaporin Z
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/29);  total (33/29)

GGATCACCAACAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTTTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCATAAACCAATAGTGACCGCCGGGTTAAAATG  >  NC_000913/915600‑915867                                                                                                                                   |                                                                                                                                          ggATCACCAACAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAATACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACt                                                                                                                                   <  1:882371/139‑1 (MQ=255)     cACCAACAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTgg                                                                                                                                 >  2:1222052/1‑137 (MQ=255)     cACCAACAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTgg                                                                                                                                 <  1:1222052/137‑1 (MQ=255)      aCCAACAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCaa                                                                                                                              <  1:1637870/139‑1 (MQ=255)                aaCCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATAcag                                                                                                                    >  1:12012/1‑139 (MQ=255)                      cACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACt                                                                                                                                   <  1:521407/118‑1 (MQ=255)                      cACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACt                                                                                                                                   >  2:521407/1‑118 (MQ=255)                      cACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAgcgc                                                                                                              >  2:483897/1‑139 (MQ=255)                         tCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGcgccgc                                                                                                           <  1:483897/139‑1 (MQ=255)                            aTACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGc                                                                                                        >  1:1380595/1‑139 (MQ=255)                                 aGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGcaacaa                                                                                                   >  2:236299/1‑139 (MQ=255)                                   ttCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCATCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATa                                                                                                 >  2:1351801/1‑139 (MQ=255)                                              gcgcGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGa                                                                                          <  2:223497/135‑1 (MQ=255)                                              gcgcGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGa                                                                                          >  1:223497/1‑135 (MQ=255)                                               cgcgGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCaa                                                                                                                              <  2:1394349/98‑1 (MQ=255)                                               cgcgGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCaa                                                                                                                              >  1:1394349/1‑98 (MQ=255)                                                 cgGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCaa                                                                                                                              <  2:1621284/96‑1 (MQ=255)                                                 cgGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCaa                                                                                                                              >  1:1621284/1‑96 (MQ=255)                                                  ggAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCAc                                                                                                                                    >  2:1396190/1‑89 (MQ=255)                                                  ggAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCAc                                                                                                                                    <  1:1396190/89‑1 (MQ=255)                                                          tGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTgg                                                                                                                                 <  1:5448/84‑1 (MQ=255)                                                          tGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTgg                                                                                                                                 >  2:1774359/1‑84 (MQ=255)                                                          tGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTgg                                                                                                                                 >  2:5448/1‑84 (MQ=255)                                                          tGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTgg                                                                                                                                 <  1:1774359/84‑1 (MQ=255)                                                             aaTAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTg                                                                                   <  1:1356666/127‑1 (MQ=255)                                                             aaTAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTg                                                                                   >  2:1356666/1‑127 (MQ=255)                                                                  ccGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCaa                                                                  >  2:835729/1‑139 (MQ=255)                                                                      cTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGt                                                                        <  1:814756/129‑1 (MQ=255)                                                                      cTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGt                                                                        >  2:814756/1‑129 (MQ=255)                                                                       tGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGAct                                                             >  1:106562/1‑139 (MQ=255)                                                                         gTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGActtc                                                           >  1:1416551/1‑139 (MQ=255)                                                                             aTGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCtttt                                                       >  1:950156/1‑139 (MQ=255)                                                                              tGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTg                                                                                   >  2:568022/1‑110 (MQ=255)                                                                              tGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTg                                                                                   <  1:568022/110‑1 (MQ=255)                                                                                   gCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGa                                                 >  1:186890/1‑139 (MQ=255)                                                                                             ttAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACt                                                                                                                                   <  1:1332551/47‑1 (MQ=255)                                                                                             ttAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACt                                                                                                                                   >  2:1332551/1‑47 (MQ=255)                                                                                             ttAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAACTACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGt                                                                        <  1:1187085/106‑1 (MQ=255)                                                                                             ttAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGt                                                                        >  2:1187085/1‑106 (MQ=255)                                                                                               aGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATa                                                                                                 <  1:1066129/79‑1 (MQ=255)                                                                                               aGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATa                                                                                                 >  2:1066129/1‑79 (MQ=255)                                                                                               aGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCa                                                                               <  2:275387/97‑1 (MQ=255)                                                                                               aGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCa                                                                               >  1:275387/1‑97 (MQ=255)                                                                                               aGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGAAACAATACCGCCGACAACATGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCa                                     <  1:236299/139‑1 (MQ=255)                                                                                                    cAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATAc                                                                                                <  2:473127/75‑1 (MQ=255)                                                                                                    cAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATAc                                                                                                >  1:473127/1‑75 (MQ=255)                                                                                                    cAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGcc                                      >  2:255252/1‑133 (MQ=255)                                                                                                    cAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGcc                                      <  1:255252/133‑1 (MQ=255)                                                                                                    cAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCa                                <  2:186890/139‑1 (MQ=255)                                                                                                    cAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCa                                >  1:319472/1‑139 (MQ=255)                                                                                                    cAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCa                                <  2:319472/139‑1 (MQ=255)                                                                                                    cAAAACCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCa                                <  2:950156/139‑1 (MQ=255)                                                                                                     aaaaCCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGc                                                                                                        <  2:1229525/66‑1 (MQ=255)                                                                                                     aaaaCCGCTGACTACCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGc                                                                                                        >  1:1229525/1‑66 (MQ=255)                                                                                                      aaaCCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAAtt                                                                                                                            >  2:1586440/1‑45 (MQ=255)                                                                                                      aaaCCGCTGGCTGCCGCGTCAAAACCCGTCTTACCACTGGCAAtt                                                                                                                            <  1:1586440/45‑1 (MQ=255)                                                                                                           gctggctgCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCATAAACCa                         >  1:537138/1‑139 (MQ=255)                                                                                                            ctggctgCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATAccgccg                                                                                           <  2:1824732/72‑1 (MQ=255)                                                                                                            ctggctgCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATAccgccg                                                                                           >  1:1824732/1‑72 (MQ=255)                                                                                                            ctggctgCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTa                                                                           >  2:1506020/1‑88 (MQ=255)                                                                                                            ctggctgCCGCGTCAAAACCCGTCTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTa                                                                           <  1:1506020/88‑1 (MQ=255)                                                                                                                               ccGTCTTACCACTGGCAATTAAATACAGCAGCGCC‑‑‑GCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCATAAACCAATAGTGACCGCCGGGTTAAAATg  <  1:720004/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                          GGATCACCAACAGGAAACCTGCACTCAATACCAGTTCAACTACCAGCGCGGAAAGCATGGAATAACCGCCTGGTGAATGCTCGCCATAACCGTTAGAAGCAAAACCGCTGGCTGCCGCGTCAAAACCCGTTTTACCACTGGCAATTAAATACAGCAGCGCCGCTGCAACAATACCGCCGACAACCTGGGCAATTACGTAGCCAACGACTTCTTTTGCCGGAAAACGTCCGCCAGCCCATAAACCAATAGTGACCGCCGGGTTAAAATG  >  NC_000913/915600‑915867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑