Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,119,307	C→T	*47* (TAG→TAA)	yceO ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,119,307	0	C	T	100.0%	205.3 / NA	65	*47* (TAG→TAA)	yceO	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/32);  total (33/32)

TCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAA  >  NC_000913/1119174‑1119438                                                                                                                                      |                                                                                                                                    tCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTATCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTaaaa                                                                                                                                >  1:1162404/1‑139 (MQ=255)    tCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATa                                                                                                                             <  2:431059/139‑1 (MQ=255)           tttAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGATTAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAAcaca                                                                                                                      <  2:1162404/139‑1 (MQ=255)            ttAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACAt                                                                                                                     <  1:827862/139‑1 (MQ=255)             tAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaaa                                                                                                                           <  2:1470333/132‑1 (MQ=255)             tAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaaa                                                                                                                           >  1:1470333/1‑132 (MQ=255)             tAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAAcaca                                                                                                                      <  2:713757/137‑1 (MQ=255)             tAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAAcaca                                                                                                                      >  1:713757/1‑137 (MQ=255)                    ttCAGCATCAGAGATGTCTGGTGAGTGCTGTATAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAAGACATagatcaga                                                                                                             >  1:1091576/1‑139 (MQ=255)                    ttCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                                 >  2:643216/1‑135 (MQ=255)                    ttCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                                 <  1:643216/135‑1 (MQ=255)                                   gTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCcaac                                                                                              >  2:703313/1‑139 (MQ=255)                                    tCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                                 >  2:411897/1‑119 (MQ=255)                                    tCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                                 <  1:411897/119‑1 (MQ=255)                                           aGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCa                                                                                      <  1:703313/139‑1 (MQ=255)                                             tGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTaaaa                                                                                                                                <  2:1158285/95‑1 (MQ=255)                                             tGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTaaaa                                                                                                                                >  1:1158285/1‑95 (MQ=255)                                             tGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCaga                                                                                    >  2:1766610/1‑139 (MQ=255)                                              gCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATACAACACATAGATCAGATCCATAATTGCCAACAATCCCCagag                                                                                   >  1:1098526/1‑139 (MQ=255)                                              gCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                                 >  2:1645403/1‑109 (MQ=255)                                              gCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                                 <  1:1645403/109‑1 (MQ=255)                                                  tAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGaaaa                                                                               >  1:296880/1‑139 (MQ=255)                                                     aaGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGa                                                                            >  1:1150431/1‑139 (MQ=255)                                                                     aTTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGaaa                                                                                <  1:1351053/119‑1 (MQ=255)                                                                     aTTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGaaa                                                                                >  2:1351053/1‑119 (MQ=255)                                                                     aTTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGAt                                                                           >  1:127724/1‑124 (MQ=255)                                                                     aTTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGAt                                                                           <  2:127724/124‑1 (MQ=255)                                                                     aTTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCt                                                            >  2:1402062/1‑139 (MQ=255)                                                                        cAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTcgc                                                         >  2:1342521/1‑139 (MQ=255)                                                                        cAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTcgc                                                         <  2:428403/139‑1 (MQ=255)                                                                         aaTTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTcgcg                                                        <  2:296880/139‑1 (MQ=255)                                                                          aTTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagatc                                                                                                                <  1:1773919/82‑1 (MQ=255)                                                                          aTTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagatc                                                                                                                >  2:1773919/1‑82 (MQ=255)                                                                           tttttCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGaa                                                      <  2:875229/139‑1 (MQ=255)                                                                             tttCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATaaa                                                                        <  2:957338/119‑1 (MQ=255)                                                                             tttCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATaaa                                                                        >  1:957338/1‑119 (MQ=255)                                                                               tCACGCTAAATTAAAGGGAGATTAAATTTTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAAtatt                                                  <  1:635621/139‑1 (MQ=255)                                                                                    cTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtga                                             <  2:1150431/139‑1 (MQ=255)                                                                                    cTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtga                                             <  1:1766610/139‑1 (MQ=255)                                                                                       aaTGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtgatga                                          >  1:1018993/1‑139 (MQ=255)                                                                                                 agaTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                                 >  2:1403560/1‑58 (MQ=255)                                                                                                 agaTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATagat                                                                                                                 <  1:1403560/58‑1 (MQ=255)                                                                                                   aTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTcgc                                                         <  1:1230258/112‑1 (MQ=255)                                                                                                   aTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTagc                                                         >  2:1230258/1‑112 (MQ=255)                                                                                                   aTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGAAACa                              >  1:1330804/1‑139 (MQ=255)                                                                                                       aaTATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCg                          <  2:1330804/139‑1 (MQ=255)                                                                                                        atatTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGa                         <  1:1342521/139‑1 (MQ=255)                                                                                                              gTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAata                                                    >  2:989705/1‑106 (MQ=255)                                                                                                              gTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAata                                                    <  1:989705/106‑1 (MQ=255)                                                                                                                   tttATGTCAGTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCCTAATTGCCAACAATCCCCAGAGAAAAAGTTAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATa              <  2:1091576/139‑1 (MQ=255)                                                                                                                      aTGTCAGTGCGGAAAATTAAAAATAAAAGACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTc           <  2:1098526/139‑1 (MQ=255)                                                                                                                            gTGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTaa     >  2:1285287/1‑139 (MQ=255)                                                                                                                             tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTc                                                           >  1:844956/1‑84 (MQ=255)                                                                                                                             tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTc                                                           <  2:844956/84‑1 (MQ=255)                                                                                                                             tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGAt                                       >  1:762249/1‑104 (MQ=255)                                                                                                                             tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGAt                                       <  2:762249/104‑1 (MQ=255)                                                                                                                             tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATa              <  2:406163/129‑1 (MQ=255)                                                                                                                             tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATa              >  1:406163/1‑129 (MQ=255)                                                                                                                             tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTaaa    >  2:1764928/1‑139 (MQ=255)                                                                                                                             tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTaaa    >  2:132758/1‑139 (MQ=255)                                                                                                                             tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTaaa    >  2:1327422/1‑139 (MQ=255)                                                                                                                             tGCGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTaaa    >  1:1643681/1‑139 (MQ=255)                                                                                                                               cGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGaa                                                      <  1:1634308/87‑1 (MQ=255)                                                                                                                               cGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGaa                                                      >  2:1634308/1‑87 (MQ=255)                                                                                                                               cGGAAAATTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTaaaaa  <  2:1018993/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                    TCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAA  >  NC_000913/1119174‑1119438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑