Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,252,961 A→G A632A (GCA→GCG dhaR → dhaKLM operon transcription activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,252,9610AG98.7% 270.8 / ‑3.6 75A632A (GCA→GCGdhaRdhaKLM operon transcription activator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (38/36);  minor base T (0/1);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.23e-01

ATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGA  >  NC_000913/1252826‑1253095
                                                                                                                                       |                                                                                                                                      
aTTAGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCgggg                                                                                                                                     <  2:509640/139‑1 (MQ=255)
 tttGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGc                                                                                                                                    <  2:135036/139‑1 (MQ=255)
   tGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAg                                                                                                                                  >  2:1308110/1‑139 (MQ=255)
    gCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGt                                                                                                                                 >  1:513552/1‑139 (MQ=255)
         aGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCTGGGCAGTTTAAg                                                                                                                            <  1:160749/139‑1 (MQ=255)
           ttGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAgcg                                                                                                                          <  1:1308110/139‑1 (MQ=255)
             gAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGcc                                                                                                                        <  1:99847/139‑1 (MQ=255)
                     ggCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATg                                                                                                                 >  2:1646396/1‑138 (MQ=255)
                     ggCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATg                                                                                                                 <  1:1646396/138‑1 (MQ=255)
                      gCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGACTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGaa                                                                                                               >  1:1131409/1‑139 (MQ=255)
                                    aGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGAtt                                                                                                 >  2:439212/1‑139 (MQ=255)
                                     gCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGAttt                                                                                                >  2:1546017/1‑139 (MQ=255)
                                      cccAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGTCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTc                                                                                               >  2:721924/1‑139 (MQ=255)
                                      cccAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGaaa                                                                                                      >  1:1706880/1‑132 (MQ=255)
                                      cccAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGaaa                                                                                                      <  2:1706880/132‑1 (MQ=255)
                                      cccAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTc                                                                                               >  2:26503/1‑139 (MQ=255)
                                      cccAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTc                                                                                               <  2:513552/139‑1 (MQ=255)
                                      cccAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTc                                                                                               >  2:148797/1‑139 (MQ=255)
                                        cAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGTCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTGTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTg                                                                                             <  1:721924/139‑1 (MQ=255)
                                         aGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGa                                                                                            <  1:439212/139‑1 (MQ=255)
                                             cacaGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACa                                                                                        <  2:421174/139‑1 (MQ=255)
                                              acaGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATa                                                                                          <  1:247057/136‑1 (MQ=255)
                                              acaGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATa                                                                                          >  2:247057/1‑136 (MQ=255)
                                                aGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGa                                                                                                            >  1:194850/1‑116 (MQ=255)
                                                aGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGa                                                                                                            <  2:194850/116‑1 (MQ=255)
                                                aGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCa                                                                                     <  1:148797/139‑1 (MQ=255)
                                                aGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCa                                                                                     >  1:20421/1‑139 (MQ=255)
                                                  gCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCaga                                                                                   <  1:1566217/139‑1 (MQ=255)
                                                  gCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCaga                                                                                   <  1:26503/139‑1 (MQ=255)
                                                     gTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGc                                                                                                                                    >  2:502038/1‑87 (MQ=255)
                                                     gTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGc                                                                                                                                    <  1:502038/87‑1 (MQ=255)
                                                     gTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTg                                                                                <  2:20421/139‑1 (MQ=255)
                                                     gTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTg                                                                                >  2:1357292/1‑139 (MQ=255)
                                                     gTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTg                                                                                <  2:1626317/139‑1 (MQ=255)
                                                     gTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTg                                                                                >  2:798260/1‑139 (MQ=255)
                                                      tCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAgcg                                                                                                                          <  2:1796392/96‑1 (MQ=255)
                                                      tCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAgcg                                                                                                                          >  1:1796392/1‑96 (MQ=255)
                                                                        ggCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATa                                                                                          >  2:1783224/1‑110 (MQ=255)
                                                                        ggCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATa                                                                                          <  1:1783224/110‑1 (MQ=255)
                                                                          cTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGgcgc                                                           >  2:884471/1‑139 (MQ=255)
                                                                              aCTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCGACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCAccc                                                       <  1:1100322/139‑3 (MQ=255)
                                                                                 ttcattttccgccACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCgg                                                              <  1:691850/117‑1 (MQ=255)
                                                                                 ttcattttccgccACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCgg                                                              >  2:691850/13‑129 (MQ=255)
                                                                                       cGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAgcg                                                                                                                          <  2:1066126/63‑1 (MQ=255)
                                                                                       cGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAgcg                                                                                                                          >  1:1066126/1‑63 (MQ=255)
                                                                                       cGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGAtt                                                                                                 <  2:1190954/88‑1 (MQ=255)
                                                                                       cGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGAtt                                                                                                 >  1:1190954/1‑88 (MQ=255)
                                                                                        ggCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAAtttt                                             >  1:657753/1‑139 (MQ=255)
                                                                                         gCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATc                                                                                      >  2:999998/1‑97 (MQ=255)
                                                                                         gCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATc                                                                                      <  1:999998/97‑1 (MQ=255)
                                                                                         gCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTc                                            >  2:673277/1‑139 (MQ=255)
                                                                                                  cGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCgggg                                                                                                                                     <  2:52466/41‑1 (MQ=255)
                                                                                                  cGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCgggg                                                                                                                                     >  1:52466/1‑41 (MQ=255)
                                                                                                  cGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGAt                                                                                           <  1:338023/83‑1 (MQ=255)
                                                                                                  cGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGAt                                                                                           >  2:338023/1‑83 (MQ=255)
                                                                                                       tgGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTg                                                                                <  2:515353/89‑1 (MQ=255)
                                                                                                       tgGCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTg                                                                                >  1:515353/1‑89 (MQ=255)
                                                                                                         gCGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGc                            <  2:1689411/139‑1 (MQ=255)
                                                                                                          cGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAggg                             <  1:127319/137‑1 (MQ=255)
                                                                                                          cGGAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAggg                             >  2:127319/1‑137 (MQ=255)
                                                                                                            gAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTGAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTg                         >  2:254616/1‑139 (MQ=255)
                                                                                                            gAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCt                                                                            >  2:187758/1‑88 (MQ=255)
                                                                                                            gAAAATGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCt                                                                            <  1:187758/88‑1 (MQ=255)
                                                                                                              aaaTGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTg                                                                                             <  1:1354756/69‑1 (MQ=255)
                                                                                                              aaaTGAAGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTg                                                                                             >  2:1354756/1‑69 (MQ=255)
                                                                                                                    aGCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATccc                 >  2:297528/1‑139 (MQ=255)
                                                                                                                     gCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCg                <  1:798260/139‑1 (MQ=255)
                                                                                                                     gCAACATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCg                <  2:1250509/139‑1 (MQ=255)
                                                                                                                       aaCATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCa                                        <  1:1307313/113‑1 (MQ=255)
                                                                                                                       aaCATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCa                                        >  2:1307313/1‑113 (MQ=255)
                                                                                                                       aaCATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGACACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCa              <  2:1131409/139‑1 (MQ=255)
                                                                                                                         cATGGCATTGTTGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCa                                        >  1:748380/1‑111 (MQ=255)
                                                                                                                         cATGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCa                                        <  2:748380/111‑1 (MQ=255)
                                                                                                                          aTGGCATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACtt           <  1:673277/139‑1 (MQ=255)
                                                                                                                              cATTGATGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTaaaa       >  2:1503744/1‑139 (MQ=255)
                                                                                                                                   aTGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGa  >  1:505403/1‑139 (MQ=255)
                                                                                                                                   aTGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGa  >  2:861205/1‑139 (MQ=255)
                                                                                                                                   aTGCGGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGa  >  2:1668467/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                      
ATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGA  >  NC_000913/1252826‑1253095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: