Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 150,947 T→C T3A (ACT→GCT)  yadC ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913150,9470TC100.0% 262.3 / NA 75T3A (ACT→GCT) yadCputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/35);  total (40/35)

TCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTT  >  NC_000913/150818‑151078
                                                                                                                                 |                                                                                                                                   
tcttgggggCAGTTGGGGGGCCTTGATAGTTTTCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCAtttt                                                                                                                            <  2:1628676/132‑1 (MQ=255)
          aGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCTAATg                                                                                                                  <  1:958928/139‑1 (MQ=255)
          aGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATg                                                                                                                  <  2:522972/139‑1 (MQ=255)
            tGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGtt                                                                                                                >  1:1087344/1‑139 (MQ=255)
              gAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTc                                                                                                                                 >  2:1281900/1‑120 (MQ=255)
              gAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTc                                                                                                                                 <  1:1281900/120‑1 (MQ=255)
                 gggCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACACTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCttt                                                                                                           >  2:353553/1‑139 (MQ=255)
                  ggCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTa                                                                                                          <  2:1087344/139‑1 (MQ=255)
                                       cTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTg                                                                                             >  1:889512/1‑131 (MQ=255)
                                       cTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTg                                                                                             <  2:889512/131‑1 (MQ=255)
                                                 tCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCtt                                                                                                            <  2:1203522/106‑1 (MQ=255)
                                                 tCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCtt                                                                                                            >  1:1203522/1‑106 (MQ=255)
                                                  cccACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCAtttt                                                                                                                            <  2:18766/89‑1 (MQ=255)
                                                  cccACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCAtttt                                                                                                                            >  1:18766/1‑89 (MQ=255)
                                                      caATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgt                                                                      <  1:852215/139‑1 (MQ=255)
                                                      caATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgt                                                                      >  2:431082/1‑139 (MQ=255)
                                                              cTTGTATATGCACTGACTTTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAata                                                              <  1:431082/139‑1 (MQ=255)
                                                                 gTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCtt                                                                                                                                  >  2:824649/1‑68 (MQ=255)
                                                                 gTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCtt                                                                                                                                  <  1:824649/68‑1 (MQ=255)
                                                                 gTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCa                                                                                                                     <  1:240415/81‑1 (MQ=255)
                                                                 gTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCa                                                                                                                     >  2:240415/1‑81 (MQ=255)
                                                                 gTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTg                                                                                             <  2:1304092/105‑1 (MQ=255)
                                                                 gTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTg                                                                                             >  1:1304092/1‑105 (MQ=255)
                                                                   atatGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCAc                                                         <  2:698239/139‑1 (MQ=255)
                                                                       gCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAac                                                     >  2:37249/1‑139 (MQ=255)
                                                                         actgactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAacac                                                   >  2:129137/1‑139 (MQ=255)
                                                                          ctgactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAAcaca                                                  <  2:1746407/139‑1 (MQ=255)
                                                                           tgactgTATTACAACGAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTGTGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACAt                                                 >  1:1224202/1‑139 (MQ=255)
                                                                           tgactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCtt                                                                                                            >  1:1291822/1‑80 (MQ=255)
                                                                           tgactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCtt                                                                                                            <  2:1291822/80‑1 (MQ=255)
                                                                           tgactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACAt                                                 <  1:129137/139‑1 (MQ=255)
                                                                            gactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTgcg                                                                                           >  2:359563/1‑96 (MQ=255)
                                                                            gactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTgcg                                                                                           <  1:359563/96‑1 (MQ=255)
                                                                            gactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATg                                                >  1:722079/1‑139 (MQ=255)
                                                                             actgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATgg                                               <  2:722079/139‑1 (MQ=255)
                                                                                 tATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCCATGTTTCTTTATTGTTATGGGTTGCGCTGTTTTTGTTTGTTATTCgt                                                                      >  2:759013/1‑112 (MQ=255)
                                                                                 tATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTc                                                                        >  1:1763567/1‑110 (MQ=255)
                                                                                 tATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTc                                                                        <  2:1763567/110‑1 (MQ=255)
                                                                                 tATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgt                                                                      <  1:759013/112‑1 (MQ=255)
                                                                                       aacaaGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGtt                                     >  2:243330/1‑139 (MQ=255)
                                                                                       aacaaGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCAAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCTTAAGtt                                     >  1:1386168/1‑139 (MQ=255)
                                                                                           aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCg                                                                       >  1:1246487/1‑101 (MQ=255)
                                                                                           aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCg                                                                       <  2:1246487/101‑1 (MQ=255)
                                                                                           aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATc                                                            <  1:579885/112‑1 (MQ=255)
                                                                                           aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATc                                                            >  2:579885/1‑112 (MQ=255)
                                                                                           aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc                                 >  1:989213/1‑139 (MQ=255)
                                                                                           aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc                                 <  1:37249/139‑1 (MQ=255)
                                                                                           aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc                                 >  2:1672495/1‑139 (MQ=255)
                                                                                             aaTACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc                                 <  1:731894/137‑1 (MQ=255)
                                                                                             aaTACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc                                 >  2:731894/1‑137 (MQ=255)
                                                                                                 cAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAg                               <  1:1122701/135‑1 (MQ=255)
                                                                                                 cAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTCGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAg                               >  2:1122701/1‑135 (MQ=255)
                                                                                                    tGCTAAAAAAAGAATGTGCCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTgcg                                                                                           <  1:1206558/72‑1 (MQ=255)
                                                                                                    tGCTAAAAAAAGAATGTATCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTgcg                                                                                           >  2:1206558/1‑72 (MQ=255)
                                                                                                    tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGtt                                                                                                     <  2:209758/62‑1 (MQ=255)
                                                                                                    tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGtt                                                                                                     >  1:209758/1‑62 (MQ=255)
                                                                                                    tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACGAGATGTAac                        >  1:1252759/1‑139 (MQ=255)
                                                                                                    tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTG‑TTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAaca                       >  1:393843/1‑139 (MQ=255)
                                                                                                     gCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTa                          <  2:1429921/136‑1 (MQ=255)
                                                                                                     gCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTa                          >  1:1429921/1‑136 (MQ=255)
                                                                                                      cTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAacac                      >  2:894794/1‑139 (MQ=255)
                                                                                                      cTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAacac                      >  2:1243223/1‑139 (MQ=255)
                                                                                                       tAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTa                          >  2:966917/1‑134 (MQ=255)
                                                                                                       tAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTa                          <  1:966917/134‑1 (MQ=255)
                                                                                                       tAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACt                     >  1:834707/1‑139 (MQ=255)
                                                                                                       tAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACt                     >  1:179167/1‑139 (MQ=255)
                                                                                                       tAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACt                     <  1:243330/139‑1 (MQ=255)
                                                                                                         aaaaaaGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATcc                                                           <  2:1385618/99‑1 (MQ=255)
                                                                                                         aaaaaaGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATcc                                                           >  1:1385618/1‑99 (MQ=255)
                                                                                                            aaaGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATg                                                                                                                  <  2:743098/41‑1 (MQ=255)
                                                                                                            aaaGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATg                                                                                                                  >  1:743098/1‑41 (MQ=255)
                                                                                                            aaaGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATg                <  2:834707/139‑1 (MQ=255)
                                                                                                             aaGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGc               <  2:179167/139‑1 (MQ=255)
                                                                                                              aGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCa              >  2:1816328/1‑139 (MQ=255)
                                                                                                                          aaGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGAttttt  >  1:263684/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                                   
TCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTT  >  NC_000913/150818‑151078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑