Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,466,996	T→C	pseudogene (1605/2513 nt)	ydbA →	pseudogene, autotransporter homolog, interrupted by IS2 and IS30

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,466,996	0	T	C	100.0%	211.0 / NA	59	pseudogene (1605/2513 nt)	ydbA	pseudogene, autotransporter homolog, interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/31);  total (28/31)

GCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCG  >  NC_000913/1466871‑1467126                                                                                                                              |                                                                                                                                   gCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTTATGGAAAAGATTCCACGGGCACCAAAATCGCgg                                                                                                                       <  1:711084/139‑1 (MQ=255)      cGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAat                                                                                                                  >  1:976946/1‑139 (MQ=255)           aTAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCgg                                                                                                             <  2:1215115/139‑1 (MQ=255)               cGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCAtt                                                                                                         <  1:313750/139‑1 (MQ=255)               cGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCAtt                                                                                                         >  1:1451328/1‑139 (MQ=255)                  aGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCa                                                                                                                                >  1:156694/1‑113 (MQ=255)                  aGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCa                                                                                                                                <  2:156694/113‑1 (MQ=255)                   ggCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTaa                                                                                                     <  2:428848/139‑1 (MQ=255)                       agagCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCt                                                                                                 >  2:539968/1‑139 (MQ=255)                        gagCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTg                                                                                                >  2:1030800/1‑139 (MQ=255)                                     aaTGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGa                                                                                   <  2:1335895/139‑1 (MQ=255)                                       tGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCgg                                                                                                                       >  2:471414/1‑101 (MQ=255)                                       tGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCgg                                                                                                                       <  1:471414/101‑1 (MQ=255)                                          cggcaccggcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCa                                                                                                                                <  1:375458/89‑1 (MQ=255)                                          cggcaccggcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCa                                                                                                                                >  2:375458/1‑89 (MQ=255)                                          cggcaccggcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGtt                                                                              >  1:1460930/1‑139 (MQ=255)                                          cggcaccggcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGtt                                                                              >  1:610964/1‑139 (MQ=255)                                                cggcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGc                                                                        <  2:1451328/139‑1 (MQ=255)                                                cggcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGc                                                                        <  1:1030800/139‑1 (MQ=255)                                                       cAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTgcgc                                                                 <  1:358660/139‑1 (MQ=255)                                                          aTCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATg                                                              <  2:487843/139‑1 (MQ=255)                                                             aaCGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCgg                                                                                                                       <  2:1090004/79‑1 (MQ=255)                                                             aaCGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCgg                                                                                                                       >  1:1090004/1‑79 (MQ=255)                                                                  tAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTgag                                                      >  2:270391/1‑139 (MQ=255)                                                                   aaCGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCAAGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTgaga                                                     <  2:976946/139‑1 (MQ=255)                                                                    acgacgCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATCTCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGaa                                                    >  1:1161556/1‑139 (MQ=255)                                                                    acgacgCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCt                                                                                                 <  1:1405099/94‑1 (MQ=255)                                                                    acgacgCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCt                                                                                                 >  2:1405099/1‑94 (MQ=255)                                                                         gCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTg                                               >  2:1636679/1‑139 (MQ=255)                                                                           cACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGACACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGt                                             <  2:1161556/139‑1 (MQ=255)                                                                             ccGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCgg                                                                                                                       <  1:1053242/63‑1 (MQ=255)                                                                             ccGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCgg                                                                                                                       >  2:1053242/1‑63 (MQ=255)                                                                                cgAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACaa                                        >  1:1014770/1‑139 (MQ=255)                                                                                cgAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACaa                                        >  1:343814/1‑139 (MQ=255)                                                                                     aaCAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCa                                   <  2:1014770/139‑1 (MQ=255)                                                                                     aaCAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCa                                   <  2:343814/139‑1 (MQ=255)                                                                                     aaCAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCa                                   <  1:18017/139‑1 (MQ=255)                                                                                     aaCAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCa                                   <  2:1367390/139‑1 (MQ=255)                                                                                     aaCAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCa                                   <  1:1636679/139‑1 (MQ=255)                                                                                            gAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTaa                            <  1:539968/139‑1 (MQ=255)                                                                                               aaaCCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGa                                                                                   >  1:59079/1‑81 (MQ=255)                                                                                               aaaCCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGa                                                                                   <  2:59079/81‑1 (MQ=255)                                                                                                 aCCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAacaaca                       >  2:699310/1‑139 (MQ=255)                                                                                                    aCTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCgg                                                                                                                       <  2:970640/40‑1 (MQ=255)                                                                                                    aCTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCgg                                                                                                                       >  1:970640/1‑40 (MQ=255)                                                                                                    aCTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCg                                                                       >  1:1282914/1‑88 (MQ=255)                                                                                                    aCTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCg                                                                       <  2:1282914/88‑1 (MQ=255)                                                                                                      tGTTGATGGAAAAGATTCCACGGGCACTAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTgc                                                                   >  1:1092571/1‑90 (MQ=255)                                                                                                      tGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTgc                                                                   <  2:1092571/90‑1 (MQ=255)                                                                                                        ttGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGTGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGgaga                >  1:1190293/1‑139 (MQ=255)                                                                                                                aaaGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGt                                                                                          <  2:1491745/57‑1 (MQ=255)                                                                                                                aaaGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGt                                                                                          >  1:1491745/1‑57 (MQ=255)                                                                                                                  aGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGc                                                                        >  2:1056569/1‑73 (MQ=255)                                                                                                                  aGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGc                                                                        <  1:1056569/73‑1 (MQ=255)                                                                                                                  aGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTgttgtt      >  2:475358/1‑139 (MQ=255)                                                                                                                  aGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTgttgtt      >  2:1774991/1‑139 (MQ=255)                                                                                                                    aTTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGTGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTc    <  2:1190293/139‑1 (MQ=255)                                                                                                                    aTTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTc    <  1:270391/139‑1 (MQ=255)                                                                                                                      tCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCg  >  1:1767411/1‑139 (MQ=255)                                                                                                                              |                                                                                                                                   GCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCG  >  NC_000913/1466871‑1467126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑