Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	160,782	C→T	*235* (TAG→TAA)	sfsA ←	sugar fermentation stimulation protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	160,782	0	C	T	100.0%	123.8 / NA	39	*235* (TAG→TAA)	sfsA	sugar fermentation stimulation protein A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/16);  total (23/16)

GGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGGCGCG  >  NC_000913/160646‑160915                                                                                                                                         |                                                                                                                                      gggAAAAATGAGGCTGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTttatta                                                                                                                                     <  1:1372380/139‑1 (MQ=255)                           cAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTa                                                                                                                              <  1:139816/120‑1 (MQ=255)                           cAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTa                                                                                                                              >  2:139816/1‑120 (MQ=255)                              aTGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAagag                                                                                                        >  1:276953/1‑139 (MQ=255)                                gCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTaa                                                                                                           <  2:568064/134‑1 (MQ=255)                                gCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTaa                                                                                                           >  1:568064/1‑134 (MQ=255)                                    ttCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCAt                                                                                                    <  2:1581365/137‑1 (MQ=255)                                    ttCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCAt                                                                                                    >  1:1581365/1‑137 (MQ=255)                                          aTAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTcag                                                                                            >  2:438347/1‑139 (MQ=255)                                              cAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGa                                                                                        >  2:969810/1‑139 (MQ=255)                                                   aTCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTa                                                                                                            >  1:1041510/1‑114 (MQ=255)                                                   aTCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTa                                                                                                            <  2:1041510/114‑1 (MQ=255)                                                            tAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGt                                                                          <  2:258501/139‑1 (MQ=255)                                                                           gtgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTa                                                           >  2:298173/1‑139 (MQ=255)                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTcagca                                                                                          <  1:861046/107‑1 (MQ=255)                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTcagca                                                                                          >  2:861046/1‑107 (MQ=255)                                                                                aGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcc                                                         >  1:622115/1‑136 (MQ=255)                                                                                aGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcc                                                         <  2:622115/136‑1 (MQ=255)                                                                                      aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTc                                                                                  <  1:576697/105‑1 (MQ=255)                                                                                      aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTc                                                                                  >  2:576697/1‑105 (MQ=255)                                                                                      aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGt                                                >  1:274565/1‑139 (MQ=255)                                                                                         gTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGC‑TTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAg                                            <  2:1424368/139‑1 (MQ=255)                                                                                          tATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAg                                            <  2:276953/139‑1 (MQ=255)                                                                                                  cACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGAc                                    >  2:763069/1‑139 (MQ=255)                                                                                                               tGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCg                                                                                >  1:1503647/1‑82 (MQ=255)                                                                                                               tGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCg                                                                                <  2:1503647/82‑1 (MQ=255)                                                                                                                 tAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTAt                     >  1:1728977/1‑139 (MQ=255)                                                                                                                   aaTTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCct                                                    >  2:1500964/1‑106 (MQ=255)                                                                                                                   aaTTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCct                                                    <  1:1500964/106‑1 (MQ=255)                                                                                                                   aaTTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTAttt                   >  2:767988/1‑139 (MQ=255)                                                                                                                       aaCCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGc                                                                      >  1:110603/1‑84 (MQ=255)                                                                                                                       aaCCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGc                                                                      <  2:110603/84‑1 (MQ=255)                                                                                                                       aaCCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTa                                                           >  2:1229369/1‑95 (MQ=255)                                                                                                                       aaCCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTa                                                           <  1:1229369/95‑1 (MQ=255)                                                                                                                       aaCCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCa               <  1:298173/139‑1 (MQ=255)                                                                                                                       aaCCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCa               >  1:1273987/1‑139 (MQ=255)                                                                                                                         ccAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGaa                                                                 <  2:1535492/87‑1 (MQ=255)                                                                                                                         ccAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGaa                                                                 >  1:1535492/1‑87 (MQ=255)                                                                                                                         ccAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTCTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATc             >  1:628224/1‑139 (MQ=255)                                                                                                                             ttacttacTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGAtg         >  1:986195/1‑139 (MQ=255)                                                                                                                                    ctttattACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg  <  1:767988/139‑1 (MQ=255)                                                                                                                                    ctttattACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg  <  2:986195/139‑1 (MQ=255)                                                                                                                                    ctttattACAATGTAACCGGCAGTGATTTTCTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg  <  2:628224/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      GGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGGCGCG  >  NC_000913/160646‑160915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑