Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,493,219	A→G	E250E (GAA→GAG)	trg →	methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,493,219	0	A	G	100.0%	245.7 / NA	69	E250E (GAA→GAG)	trg	methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/36);  total (33/36)

GCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAACCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGG  >  NC_000913/1493100‑1493353                                                                                                                        |                                                                                                                                        gCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGGCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGAGTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGGCGTAATGaaa                                                                                                                      >  1:710246/1‑139 (MQ=255)    cTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCg                                                                                                                   <  2:43611/139‑1 (MQ=255)      ggTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGt                                                                                                                 >  2:813580/1‑139 (MQ=255)            gACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGGAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCt                                                                                                             >  2:1358931/1‑137 (MQ=255)            gACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGGAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCt                                                                                                             <  1:1358931/137‑1 (MQ=255)            gACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTa                                                                                                           >  1:331847/1‑139 (MQ=255)              cGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAg                                                                                                         <  2:221826/139‑1 (MQ=255)              cGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAg                                                                                                         <  2:331847/139‑1 (MQ=255)                 tGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAgtcg                                                                                                      <  1:813580/139‑1 (MQ=255)                 tGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAg                                                                                                         <  2:1210729/136‑1 (MQ=255)                 tGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAg                                                                                                         >  1:1210729/1‑136 (MQ=255)                   aTAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGt                                                                                                                                >  1:863298/1‑111 (MQ=255)                   aTAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGt                                                                                                                                <  2:863298/111‑1 (MQ=255)                               gTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCCGTGGCGAACTGACGATGAATGATGAGCCGGCGGGt                                                                                                                                >  2:1368148/1‑99 (MQ=255)                               gTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGAACTGACGATGAATGATGAGCCGGCGGGt                                                                                                                                <  1:1368148/99‑1 (MQ=255)                                tGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAAt                                                                                       <  1:1335712/139‑1 (MQ=255)                                tGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAAt                                                                                       <  1:100158/139‑1 (MQ=255)                                 gCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggcg                                                                                                                                   >  1:136884/1‑94 (MQ=255)                                 gCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggcg                                                                                                                                   <  2:136884/94‑1 (MQ=255)                                 gCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATg                                                                                      >  1:368149/1‑139 (MQ=255)                                  cTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgc                                                                                     >  1:1562866/1‑139 (MQ=255)                                  cTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgc                                                                                     >  1:1220255/1‑139 (MQ=255)                                  cTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgc                                                                                     >  1:1835655/1‑139 (MQ=255)                                      gTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgcagca                                                                                 <  2:1296099/139‑1 (MQ=255)                                            tCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACt                                                                           <  2:655450/139‑1 (MQ=255)                                               tCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGc                                                                                                              <  2:1393151/101‑1 (MQ=255)                                               tCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGc                                                                                                              >  1:1393151/1‑101 (MQ=255)                                               tCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑ggg                                                                       >  2:1620932/1‑139 (MQ=255)                                               tCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑ggg                                                                       <  2:368149/139‑1 (MQ=255)                                                   tCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGATg                                                                   <  2:1562866/139‑1 (MQ=255)                                                   tCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGATg                                                                   <  2:1835655/139‑1 (MQ=255)                                                   tCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGATg                                                                   <  2:411928/139‑1 (MQ=255)                                                             cAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCAtt                                                                                                 >  1:196753/1‑100 (MQ=255)                                                             cAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCAtt                                                                                                 <  2:196753/100‑1 (MQ=255)                                                             cAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGATg                                                                   >  1:110488/1‑129 (MQ=255)                                                             cAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGATg                                                                   <  2:110488/129‑1 (MQ=255)                                                             cAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGAGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGATGACAGTAGGGa                                                         >  2:1380652/1‑139 (MQ=255)                                                                 aTGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGATGACAGTAGGGACTGt                                                     <  1:1421167/139‑1 (MQ=255)                                                                  tGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCGGCATTCACT‑GGGGATg                                                                   >  2:824556/1‑124 (MQ=255)                                                                  tGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCGGCATTCACT‑GGGGATg                                                                   <  1:824556/124‑1 (MQ=255)                                                                          aaCGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGt                                                                                                                 <  1:842096/71‑1 (MQ=255)                                                                          aaCGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGt                                                                                                                 >  2:842096/1‑71 (MQ=255)                                                                            cGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgcagca                                                                                 >  2:283580/1‑101 (MQ=255)                                                                            cGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgcagca                                                                                 <  1:283580/101‑1 (MQ=255)                                                                            cGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCAtt                                                                               >  1:1792075/1‑103 (MQ=255)                                                                            cGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCAtt                                                                               <  2:1792075/103‑1 (MQ=255)                                                                               aTTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGAt                                                                    <  2:1701343/110‑1 (MQ=255)                                                                               aTTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGAt                                                                    >  1:1701343/1‑110 (MQ=255)                                                                               aTTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGATGACAGTAGGGACTGTTCg                                                  <  1:43583/128‑1 (MQ=255)                                                                               aTTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGATGACAGTAGGGACTGTTCg                                                  >  2:43583/1‑128 (MQ=255)                                                                               aTTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGATGACAGTAGGGACTGTTCGACAGGGCGCgg                                       >  2:429435/1‑139 (MQ=255)                                                                                 tGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGAt                                                                    <  2:1562312/108‑1 (MQ=255)                                                                                 tGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGAt                                                                    >  1:1562312/1‑108 (MQ=255)                                                                                 tGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGaa                                     <  2:1220255/139‑1 (MQ=255)                                                                                       aaTCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGAtt                               <  1:988384/139‑1 (MQ=255)                                                                                        aTCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGAttt                              <  1:429435/139‑1 (MQ=255)                                                                                         tCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACt                                                                           <  2:471696/94‑1 (MQ=255)                                                                                         tCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACt                                                                           >  1:471696/1‑94 (MQ=255)                                                                                         tCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTa                             >  2:888867/1‑139 (MQ=255)                                                                                                          aCGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGaaa            <  1:1149897/139‑1 (MQ=255)                                                                                                            gATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGc                                                                                           >  2:1547594/1‑59 (MQ=255)                                                                                                            gATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGc                                                                                           <  1:1547594/59‑1 (MQ=255)                                                                                                            gATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgca                                                                                    >  2:1703016/1‑66 (MQ=255)                                                                                                            gATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgca                                                                                    <  1:1703016/66‑1 (MQ=255)                                                                                                                    atgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTg   >  1:1316693/1‑139 (MQ=255)                                                                                                                    atgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAg                 >  2:1201967/1‑124 (MQ=255)                                                                                                                    atgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAg                 <  1:1201967/124‑1 (MQ=255)                                                                                                                    atgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTgg  >  2:231810/1‑139 (MQ=255)                                                                                                                    atgaGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTgg  >  2:1676777/1‑139 (MQ=255)                                                                                                                        |                                                                                                                                        GCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAACCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACT‑GGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGG  >  NC_000913/1493100‑1493353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑