Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,503,125	G→A	*223* (TAG→TAA)	ydcL →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,503,125	0	G	A	100.0%	146.3 / NA	46	*223* (TAG→TAA)	ydcL	lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/20);  total (26/20)

AAAGTCGTTCGTCAGGGCGAAGGTAAAGACCTGAATAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAGTTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGTTTGCTGTTGTTCAATGTATTGGCGG  >  NC_000913/1502997‑1503254                                                                                                                                 |                                                                                                                                  aaaGTCGTTCGTCAGGGCGAAGGTAAAGACCTGAATAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGAcgc                                                                                                                         <  2:41158/139‑1 (MQ=255)       gttcgtCAGGGCGAAGGTAAAGACCTGAATAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGAcgcg                                                                                                                        >  2:1634312/1‑134 (MQ=255)       gttcgtCAGGGCGAAGGTAAAGACCTGAATAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGAcgcg                                                                                                                        <  1:1634312/134‑1 (MQ=255)                                  aaTAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTgcg                                                                                        >  2:1165879/1‑139 (MQ=255)                                       cGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTg                                                                                          <  1:590659/132‑1 (MQ=255)                                       cGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTg                                                                                          >  2:590659/1‑132 (MQ=255)                                                     tGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCtt                                                                      >  2:1742184/1‑138 (MQ=255)                                                     tGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCtt                                                                      <  1:1742184/138‑1 (MQ=255)                                                                 atatTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCa                                                                                                                     >  2:1512834/1‑79 (MQ=255)                                                                 atatTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCa                                                                                                                     <  1:1512834/79‑1 (MQ=255)                                                                                   atgaCATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCa                                                                           >  2:817005/1‑103 (MQ=255)                                                                                   atgaCATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCa                                                                           <  1:817005/103‑1 (MQ=255)                                                                                     gaCATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGgcgc                                                                                                       <  1:1622452/73‑1 (MQ=255)                                                                                     gaCATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGgcgc                                                                                                       >  2:1622452/1‑73 (MQ=255)                                                                                      aCATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTg                                    >  2:1012518/1‑139 (MQ=255)                                                                                       cATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGa                                   >  1:1834809/1‑139 (MQ=255)                                                                                       cATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGa                                   >  1:758576/1‑139 (MQ=255)                                                                                        aTGGCGTCCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAggg                                                                 <  1:1169355/108‑1 (MQ=255)                                                                                        aTGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAggg                                                                 >  2:1169355/1‑108 (MQ=255)                                                                                        aTGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGAc                                  <  2:1834809/139‑1 (MQ=255)                                                                                          ggCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGgcgc                                                 <  1:1065903/122‑1 (MQ=255)                                                                                          ggCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGgcgc                                                 >  2:1065903/1‑122 (MQ=255)                                                                                                    gCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACgcgc                                                                                                                       >  2:1593889/1‑42 (MQ=255)                                                                                                    gCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACgcgc                                                                                                                       <  1:1593889/42‑1 (MQ=255)                                                                                                        ccaTGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGgcgc                                                 <  1:1796541/108‑1 (MQ=255)                                                                                                        ccaTGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGgcgc                                                 >  2:1796541/1‑108 (MQ=255)                                                                                                             tttGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGgcgc                                                                                                       >  2:1459913/1‑49 (MQ=255)                                                                                                             tttGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGgcgc                                                                                                       <  1:1459913/49‑1 (MQ=255)                                                                                                             tttGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAAcccc                                                                            >  1:667694/1‑76 (MQ=255)                                                                                                             tttGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAAcccc                                                                            <  2:667694/76‑1 (MQ=255)                                                                                                             tttGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGtt                          >  1:594824/1‑126 (MQ=255)                                                                                                             tttGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGtt                          <  2:594824/126‑1 (MQ=255)                                                                                                             tttGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGTTTGCTGTTGTTCaa             >  2:1012497/1‑139 (MQ=255)                                                                                                             tttGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGTTTGCTGTTGTTCaa             >  2:1153152/1‑139 (MQ=255)                                                                                                             tttGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGTTTGCTGTTGTTCaa             >  2:1441758/1‑139 (MQ=255)                                                                                                             tttGACGTTAACAAAAAGTAATTCCAGAAGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGTTTGCTGTTGTTCaa             >  2:986226/1‑139 (MQ=255)                                                                                                                      aaCAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGTTTGCTGTTGTTCAATGTATTGGc    >  2:180951/1‑139 (MQ=255)                                                                                                                      aaCAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCAACTGACCAGGGGTTTGCTGTTGTTCAATGTATTGGc    >  1:1387353/1‑139 (MQ=255)                                                                                                                      aaCAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATACTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAg                               >  2:193725/1‑112 (MQ=255)                                                                                                                      aaCAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATACTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAg                               <  1:193725/112‑1 (MQ=255)                                                                                                                       ataaaaaGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCa                                                                           <  1:1250182/65‑1 (MQ=255)                                                                                                                       aCAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCa                                                                           >  2:1250182/1‑67 (MQ=255)                                                                                                                       aCAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGTTTGCTGTTGTTCaa             <  2:162724/129‑1 (MQ=255)                                                                                                                       aCAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGTTTGCTGTTGTTCaa             >  1:162724/1‑129 (MQ=255)                                                                                                                       aCAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGTTTGCTGTTGTTCAATGTATTGGCg   <  1:1153152/139‑1 (MQ=255)                                                                                                                        cAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGTTTGCTGTTGTTCAATGTATTGGCgg  <  2:758576/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                  AAAGTCGTTCGTCAGGGCGAAGGTAAAGACCTGAATAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAGTTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGTTTGCTGTTGTTCAATGTATTGGCGG  >  NC_000913/1502997‑1503254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑