Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,559,680 G→A G77G (GGC→GGT)  ddpC ← D,D‑dipeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,559,6800GA100.0% 177.2 / NA 55G77G (GGC→GGT) ddpCD,D‑dipeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/29);  total (26/29)

CACCGGATAGACATCCGAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCAC  >  NC_000913/1559553‑1559811
                                                                                                                               |                                                                                                                                   
cacCGGATAGACATCCGAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTg                                                                                                                          <  2:178458/139‑1 (MQ=255)
  ccGGATAGACATCCGAGTAGCGAACCAATCATACCCGCGATGGCAACCACGACTACTCCGGCGAGGATTGATTGCTGACTGCCGACTAGTACGCGGCTAAAGTGGTCGCGTCCCACTTCATCGGTACCAAACCAGTgcg                                                                                                                        <  1:37506/139‑1 (MQ=255)
               cGAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAggcgg                                                                                                           >  1:1292647/1‑139 (MQ=255)
                 aGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCa                                                                                                         >  2:986546/1‑139 (MQ=255)
                        aaCCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAggcg                                                                                                  >  1:675256/1‑139 (MQ=255)
                         accgctCATACCCGCAATTGCAACCACGACTAATCCGGCGAGAAGTGATTGCTGACGGCCGACCGGTACGCGGCGAAATAGATCGCGTCCCACTTCATCGGTACCAGACCAGTGCGCCGCGGAAGGCGGCAAAAgacgg                                                                                                 <  1:812222/134‑1 (MQ=255)
                                       cAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAAt                                                                                   >  2:947072/1‑139 (MQ=255)
                                        aaTGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATg                                                                                  >  2:514514/1‑139 (MQ=255)
                                        aaTGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATg                                                                                  >  1:1601132/1‑139 (MQ=255)
                                             cAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGtt                                                                             <  2:675256/139‑1 (MQ=255)
                                               aCCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTgg                                                                           >  1:823393/1‑139 (MQ=255)
                                                ccACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTggg                                                                          >  1:1007858/1‑139 (MQ=255)
                                                      cTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATg                                                                    <  2:22557/139‑1 (MQ=255)
                                                      cTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATg                                                                    >  2:1116373/1‑139 (MQ=255)
                                                         aTCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATg                                                                    >  2:226019/1‑136 (MQ=255)
                                                         aTCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATg                                                                    <  1:226019/136‑1 (MQ=255)
                                                         aTCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGt                                                                 >  2:502681/1‑139 (MQ=255)
                                                           ccGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCg                                                               >  1:639022/1‑139 (MQ=255)
                                                           ccGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCg                                                               <  2:1601132/139‑1 (MQ=255)
                                                              gCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCgcca                                                            >  2:902706/1‑139 (MQ=255)
                                                                  gaATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCgccagcca                                                        <  1:230578/139‑1 (MQ=255)
                                                                    attgattgCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACg                                                      <  2:1007858/139‑1 (MQ=255)
                                                                            cTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGaaaaa                                              <  2:639022/139‑1 (MQ=255)
                                                                                  gCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTgcg                                                                                                                        <  1:89919/59‑1 (MQ=255)
                                                                                  gCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTgcg                                                                                                                        >  2:89919/1‑59 (MQ=255)
                                                                                  gCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAatcatc                                        <  1:947072/139‑1 (MQ=255)
                                                                                      aCCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAatcatc                                        <  1:1519203/135‑1 (MQ=255)
                                                                                      aCCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAatcatc                                        >  2:1519203/1‑135 (MQ=255)
                                                                                        cAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAAt                                  <  1:514514/139‑1 (MQ=255)
                                                                                        cAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAAt                                  <  2:823393/139‑1 (MQ=255)
                                                                                        cAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAAt                                  <  1:986546/139‑1 (MQ=255)
                                                                                        cAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAAt                                  <  1:1116373/139‑1 (MQ=255)
                                                                                                  cTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCAt                                                                     <  1:1761814/94‑1 (MQ=255)
                                                                                                  cTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCAt                                                                     >  2:1761814/1‑94 (MQ=255)
                                                                                                   tAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAATAc                       <  2:1292647/139‑1 (MQ=255)
                                                                                                     aaCAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAggcgg                                                                                                 <  1:995644/63‑1 (MQ=255)
                                                                                                     aaCAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAAtcatcatca                                    <  1:1053230/124‑1 (MQ=255)
                                                                                                     aaCAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAAtcatcatca                                    >  2:1053230/1‑124 (MQ=255)
                                                                                                     aaCAGATCGCGTCCCACTTAATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAggcgg                                                                                                 >  2:995644/1‑63 (MQ=255)
                                                                                                            cgcgTCCCACTTCATCGGTACCAAACCAGTGCGCCGCa                                                                                                                   <  2:646742/38‑1 (MQ=255)
                                                                                                            cgcgTCCCACTTCATCGGTACCAAACCAGTGCGCCGCa                                                                                                                   >  1:646742/1‑38 (MQ=255)
                                                                                                            cgcgTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAggcggg                                                                                                <  2:885073/57‑1 (MQ=255)
                                                                                                            cgcgTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAggcggg                                                                                                >  1:885073/1‑57 (MQ=255)
                                                                                                            cgcgTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAATACAATGATGAc              >  1:632150/1‑139 (MQ=255)
                                                                                                               gTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAg                                                                                                               >  2:256610/1‑39 (MQ=255)
                                                                                                               gTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAg                                                                                                               <  1:256610/39‑1 (MQ=255)
                                                                                                               gTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAatcatc                                        <  2:666685/110‑1 (MQ=255)
                                                                                                               gTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAatcatc                                        >  1:666685/1‑110 (MQ=255)
                                                                                                               gTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTa                           <  2:1678643/123‑1 (MQ=255)
                                                                                                               gTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTa                           >  1:1678643/1‑123 (MQ=255)
                                                                                                                        cATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCAc  <  2:632150/139‑1 (MQ=255)
                                                                                                                        cATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCAc  <  1:902706/139‑1 (MQ=255)
                                                                                                                        cATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCAc  >  2:228366/1‑139 (MQ=255)
                                                                                                                           cGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGtt                                                                                           <  2:588653/47‑1 (MQ=255)
                                                                                                                           cGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGtt                                                                                           >  1:588653/1‑47 (MQ=255)
                                                                                                                               |                                                                                                                                   
CACCGGATAGACATCCGAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCAC  >  NC_000913/1559553‑1559811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑