Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,565,758	C→T	*461* (TAG→TAA)	dosC ←	diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,565,758	0	C	T	100.0%	159.9 / NA	50	*461* (TAG→TAA)	dosC	diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/26);  total (24/26)

TCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAAC  >  NC_000913/1565631‑1565869                                                                                                                                |                                                                                                                tCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGc                                                                                                      >  1:961761/1‑139 (MQ=255)                 gTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAaca                                                                                      <  2:961761/139‑1 (MQ=255)                 gTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAaca                                                                                      <  2:1645250/139‑1 (MQ=255)                 gTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAaca                                                                                      <  2:1023037/139‑1 (MQ=255)                             cGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCt                                                                          >  2:775635/1‑139 (MQ=255)                               cACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGTCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAaca                                                                                      <  2:1673337/125‑1 (MQ=255)                               cACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGTCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAaca                                                                                      >  1:1673337/1‑125 (MQ=255)                                    atcatATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                   >  2:1234446/1‑139 (MQ=255)                                    atcatATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                   <  1:301805/139‑1 (MQ=255)                                      catATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTg                                                                 <  2:956328/139‑1 (MQ=255)                                      catATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTg                                                                 <  2:319646/139‑1 (MQ=255)                                         atTTTGCTCAAGGGCGGGGAAAAAAATACCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCg                                                              <  2:111062/139‑1 (MQ=255)                                            ttGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGata                                                           >  2:471713/1‑139 (MQ=255)                                               cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTAGCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGtt                                                                                           >  1:1201361/1‑104 (MQ=255)                                               cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGtt                                                                                           <  2:1201361/104‑1 (MQ=255)                                                    gggCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGc                                                   >  1:121723/1‑139 (MQ=255)                                                       cGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                   >  1:517228/1‑120 (MQ=255)                                                       cGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                   <  2:517228/120‑1 (MQ=255)                                                         gggAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAacac                                                                                     <  2:758934/100‑1 (MQ=255)                                                         gggAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAacac                                                                                     >  1:758934/1‑100 (MQ=255)                                                                        tCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTAcc                                                                           >  2:1301769/1‑95 (MQ=255)                                                                        tCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTAcc                                                                           <  1:1301769/95‑1 (MQ=255)                                                                          ggcggcATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                             <  2:121723/139‑1 (MQ=255)                                                                             ggcATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTTCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGgcgc                          >  1:1224017/1‑139 (MQ=255)                                                                                    tCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAc                                                                                                          <  1:1189846/52‑1 (MQ=255)                                                                                    tCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTAcc                                                                           <  1:932262/83‑1 (MQ=255)                                                                                    tCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTAcc                                                                           >  2:932262/1‑83 (MQ=255)                                                                                    tCCGCATCAGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAc                                                                                                          >  2:1189846/1‑52 (MQ=255)                                                                                         aTCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAGGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACag                                                      <  2:1382322/99‑1 (MQ=255)                                                                                         aTCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAc                                                                                                          >  1:530522/1‑47 (MQ=255)                                                                                         aTCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAc                                                                                                          <  2:530522/47‑1 (MQ=255)                                                                                         aTCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACag                                                      >  1:1382322/1‑99 (MQ=255)                                                                                             gTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTg                                                                 <  1:70419/84‑1 (MQ=255)                                                                                             gTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTg                                                                 >  2:70419/1‑84 (MQ=255)                                                                                              ttAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                           <  2:1264484/105‑1 (MQ=255)                                                                                              ttAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                           >  1:1264484/1‑105 (MQ=255)                                                                                              ttAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGAcc         >  2:625598/1‑139 (MQ=255)                                                                                                   ttCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTgg                                                                >  1:778649/1‑79 (MQ=255)                                                                                                   ttCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTgg                                                                <  2:778649/79‑1 (MQ=255)                                                                                                     cATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAAc  <  1:775635/139‑1 (MQ=255)                                                                                                             ccTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGTGTTCAaca                                                                                      <  2:1191496/47‑1 (MQ=39)                                                                                                             ccTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAaca                                                                                      >  1:1191496/1‑47 (MQ=255)                                                                                                                   cATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGtt                                                                                 <  2:682023/46‑1 (MQ=255)                                                                                                                   cATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGtt                                                                                 >  1:682023/1‑46 (MQ=255)                                                                                                                   cATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGgcg                           >  2:309260/1‑100 (MQ=255)                                                                                                                   cATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGgcg                           <  1:309260/100‑1 (MQ=255)                                                                                                                      ccTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                             >  2:1706759/1‑95 (MQ=255)                                                                                                                      ccTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                             >  1:920980/1‑95 (MQ=255)                                                                                                                      ccTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                             <  2:920980/95‑1 (MQ=255)                                                                                                                      ccTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                             <  1:1706759/95‑1 (MQ=255)                                                                                                                                |                                                                                                                TCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAAC  >  NC_000913/1565631‑1565869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑