Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,665,120	G→A	*205* (TAG→TAA)	dmsD →	twin‑argninine leader‑binding protein for DmsA and TorA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,665,120	0	G	A	100.0%	160.9 / NA	51	*205* (TAG→TAA)	dmsD	twin‑argninine leader‑binding protein for DmsA and TorA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/27);  total (24/27)

ACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGC  >  NC_000913/1664985‑1665252                                                                                                                                        |                                                                                                                                     acGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGAt                                                                                                                                   <  2:1568209/139‑1 (MQ=255)                  ttATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCgg                                                                                                                  <  1:276780/139‑1 (MQ=255)                         aaaaGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGa                                                                                                           <  2:849538/139‑1 (MQ=255)                                       cTTTCTGCCGTGCACTGGGTGAACTGGCTCACTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAAt                                                                                             <  1:437130/139‑1 (MQ=255)                                           cTACCGGGCACTGGGTGAACTGGCTCGCTTAACGCTGGTGCAATGGAAATTACAACTGTTAATTCCTGTCGCGGTTAAACCGATGTTTCTATAAGATAACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCgg                                                                                         <  1:918586/139‑1 (MQ=255)                                            tACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGc                                                                                        <  1:583944/139‑1 (MQ=255)                                             aCCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGAt                                                                                                                                   >  1:839117/1‑95 (MQ=255)                                             aCCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGAt                                                                                                                                   <  2:839117/95‑1 (MQ=255)                                              ccGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaa                                                                                      <  2:1681423/139‑1 (MQ=255)                                                gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGAt                                                                                                                                   >  2:1519899/1‑92 (MQ=255)                                                gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGAt                                                                                                                                   <  1:1519899/92‑1 (MQ=255)                                                gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGa                                                                                                           <  1:488379/116‑1 (MQ=255)                                                gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGa                                                                                                           >  2:488379/1‑116 (MQ=255)                                                gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATcc                                                                                           <  2:1492672/132‑1 (MQ=255)                                                gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATcc                                                                                           >  1:1492672/1‑132 (MQ=255)                                                gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata                                                                                    <  2:521690/139‑1 (MQ=255)                                                gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata                                                                                    >  2:918586/1‑139 (MQ=255)                                                gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata                                                                                    >  1:1129373/1‑139 (MQ=255)                                                gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata                                                                                    <  2:1235604/139‑1 (MQ=255)                                                   cACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaataa                                                                                   >  1:326017/1‑137 (MQ=255)                                                   cACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaataa                                                                                   <  2:326017/137‑1 (MQ=255)                                                   cACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAataata                                                                                 >  2:468292/1‑139 (MQ=255)                                                      tGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCgg                                                                                                                  <  2:1344603/103‑1 (MQ=255)                                                      tGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCgg                                                                                                                  >  1:1344603/1‑103 (MQ=255)                                                        ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGATGATTACGCGCAATCCGGCaa                                                                                      <  1:295430/129‑1 (MQ=255)                                                        ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaa                                                                                      >  2:295430/1‑129 (MQ=255)                                                        ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTa                                                                            >  2:1342179/1‑139 (MQ=255)                                                             aCTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATcc                                                                                           <  1:557766/119‑1 (MQ=255)                                                             aCTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATcc                                                                                           >  2:557766/1‑119 (MQ=255)                                                                           cGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGc                                                                                                                     <  1:1137638/79‑1 (MQ=255)                                                                           cGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGc                                                                                                                     >  2:1137638/1‑79 (MQ=255)                                                                           cGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTAt                                                         >  2:43698/1‑139 (MQ=255)                                                                                        gCAATCACAACTGTTAATTCCTGTAGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAgcacg                                            <  1:1585816/139‑1 (MQ=255)                                                                                                  cTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCtttt                                  >  1:622196/1‑139 (MQ=255)                                                                                                          tCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTgg                          >  2:624747/1‑139 (MQ=255)                                                                                                            cTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGcc                        >  2:737317/1‑139 (MQ=255)                                                                                                             tGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCctc                      >  2:996284/1‑139 (MQ=255)                                                                                                              gTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCAGCTGGCctc                      >  1:1257405/1‑139 (MQ=255)                                                                                                               tCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAAt                                                                                             <  1:231610/67‑1 (MQ=255)                                                                                                               tCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAAt                                                                                             >  2:231610/1‑67 (MQ=255)                                                                                                               tCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaa                                                                                      <  1:1629992/74‑1 (MQ=255)                                                                                                               tCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaa                                                                                      >  2:1629992/1‑74 (MQ=255)                                                                                                               tCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCctct                     >  2:630577/1‑139 (MQ=255)                                                                                                                         aCCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGt                                                                                                             >  1:226173/1‑41 (MQ=255)                                                                                                                         aCCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGt                                                                                                             <  2:226173/41‑1 (MQ=255)                                                                                                                             cTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCa            <  1:186666/134‑1 (MQ=255)                                                                                                                             cTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCa            >  2:186666/1‑134 (MQ=255)                                                                                                                              tGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGt     <  1:996284/139‑1 (MQ=255)                                                                                                                                  tCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGc  <  1:43698/139‑1 (MQ=255)                                                                                                                                  tCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGc  <  1:630577/139‑1 (MQ=255)                                                                                                                                  tCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGc  <  2:622196/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     ACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGC  >  NC_000913/1664985‑1665252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑