Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,862,634	A→G	intergenic (‑205/‑137)	msrB ← / → gapA	methionine sulfoxide reductase B/glyceraldehyde‑3‑phosphate dehydrogenase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,862,634	0	A	G	100.0%	184.7 / NA	52	intergenic (‑205/‑137)	msrB/gapA	methionine sulfoxide reductase B/glyceraldehyde‑3‑phosphate dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/27);  total (25/27)

TTTGTCCTAAACTTGATCTCGACGAAATGGCTG‑CACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGAGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACAAATAGCTGGTGGAA  >  NC_000913/1862506‑1862767                                                                                                                                       |                                                                                                                                      tttGTCCTAAACTTGATCTCGACGAAATGGCTGTCCTCAAAAGCG‑GCTGAAAATCATATTTGTATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAACGGAAGAGTGGGGCGagtcat                                                                                                                             <  1:275738/139‑2 (MQ=255) tttGTCCTAAACTTGATCTCGACGAAATGGCTG‑CACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGagtcag                                                                                                                             <  2:912998/139‑1 (MQ=255)            cTTGATCTCGACGAAATGGCTG‑CACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGc                                                                                                                  <  1:1794435/139‑1 (MQ=255)              tGATCTCGACGAAATGGCTG‑CACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCtt                                                                                                                <  2:571786/139‑1 (MQ=255)                   tcGACGAAATGGCTG‑CACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGca                                                                                                           <  2:602430/139‑1 (MQ=255)                   tcGACGAAATGGCTG‑CACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGca                                                                                                           <  2:1455667/139‑1 (MQ=255)                    cgacgaAATGGCTG‑CACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGcac                                                                                                          >  1:1638732/1‑139 (MQ=255)                     gacgaAATGGCTG‑CACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGcaca                                                                                                         >  2:99241/1‑139 (MQ=255)                          aaTGGCTG‑CACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGca                                                                                                           >  1:1266363/1‑132 (MQ=255)                          aaTGGCTG‑CACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGca                                                                                                           <  2:1266363/132‑1 (MQ=255)                           aTGGCTG‑CACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGca                                                                                                           <  1:466377/131‑1 (MQ=255)                           aTGGCTG‑CACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGca                                                                                                           >  2:466377/1‑131 (MQ=255)                                   cACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTc                                                                                            >  1:1693333/1‑139 (MQ=255)                                             gTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTg                                                                                  >  1:860114/1‑139 (MQ=255)                                              tgatgaAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGagtcag                                                                                                                             <  2:417816/95‑1 (MQ=255)                                              tgatgaAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGagtcag                                                                                                                             >  1:417816/1‑95 (MQ=255)                                                atgaAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACAGGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGAt                                                                                    >  2:1706123/1‑139 (MQ=255)                                                atgaAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGaca                                                                               >  2:484115/1‑139 (MQ=255)                                                atgaAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGaca                                                                               >  2:953907/1‑139 (MQ=255)                                                     aaTCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGAtt                                                                          <  1:99241/139‑1 (MQ=255)                                                            tttttATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTg                                                                   >  1:1111163/1‑139 (MQ=255)                                                               ttATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACg                                                                <  2:1176368/139‑1 (MQ=255)                                                                    gTAATTGCCCTTTAAAATTCGGGGCGCCGACAGGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACg                                                                <  1:1706123/139‑1 (MQ=255)                                                                     tAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACg                                                                <  1:151493/133‑1 (MQ=255)                                                                     tAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACg                                                                >  2:151493/1‑133 (MQ=255)                                                                       aTTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCtt                                                                                                                >  2:1291096/1‑83 (MQ=255)                                                                       aTTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCtt                                                                                                                <  1:1291096/83‑1 (MQ=255)                                                                                          ggCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACg                                                                >  1:1671405/1‑112 (MQ=255)                                                                                          ggCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACg                                                                <  2:1671405/112‑1 (MQ=255)                                                                                          ggCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAg                                     >  2:338774/1‑139 (MQ=255)                                                                                           gcgcC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAgg                                    <  2:1824211/139‑1 (MQ=255)                                                                                           gcgcC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAgg                                    <  2:860114/139‑1 (MQ=255)                                                                                             gcC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCa                                  <  1:484115/139‑1 (MQ=255)                                                                                                          cATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTa                          <  2:1693333/139‑1 (MQ=255)                                                                                                               ggTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCAc                     <  1:197619/139‑1 (MQ=255)                                                                                                                gTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACt                    >  2:861648/1‑139 (MQ=255)                                                                                                                gTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACt                    >  1:1138953/1‑139 (MQ=255)                                                                                                                 tctcAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGaca                                                                               >  1:384751/1‑79 (MQ=255)                                                                                                                 tctcAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGaca                                                                               <  2:384751/79‑1 (MQ=255)                                                                                                                      aGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACaaa              >  2:698545/1‑139 (MQ=255)                                                                                                                      aGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACaaa              >  1:74281/1‑139 (MQ=255)                                                                                                                        cccAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACAAATa            >  1:1020264/1‑139 (MQ=255)                                                                                                                          cAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCttt                            <  1:1280298/121‑1 (MQ=255)                                                                                                                          cAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCttt                            >  2:1280298/1‑121 (MQ=255)                                                                                                                           aaaGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACAAATAGCt         >  2:1528977/1‑139 (MQ=255)                                                                                                                             aGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACAAATAGCtgg       <  1:489041/139‑1 (MQ=255)                                                                                                                               gAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACAAATAGCtggtg     <  2:74281/139‑1 (MQ=255)                                                                                                                               gAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACAAATAGCtggtg     <  1:338774/139‑1 (MQ=255)                                                                                                                               gAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACAAATAGCtggtg     <  2:1020264/139‑1 (MQ=255)                                                                                                                               gAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACAAATAGCtggtg     <  1:698545/139‑1 (MQ=255)                                                                                                                                  gagTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACAAATAGCTGGTGGaa  >  1:1529625/1‑139 (MQ=255)                                                                                                                                  gagTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACAAATAGCTGGTGGaa  >  1:1130572/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                      TTTGTCCTAAACTTGATCTCGACGAAATGGCTG‑CACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCC‑‑‑‑‑GACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGAGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACAAATAGCTGGTGGAA  >  NC_000913/1862506‑1862767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑