Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 185,465 G→A G161G (GGC→GGT)  dapD ← 2,3,4,5‑tetrahydropyridine‑2‑carboxylate N‑succinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913185,4650GA100.0% 213.6 / NA 66G161G (GGC→GGT) dapD2,3,4,5‑tetrahydropyridine‑2‑carboxylate N‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/35);  total (31/35)

ACGCCCATGGAAATGACGGAACCTTCTTCGACAATCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACGCCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGCCGATGTTGACGTAAGACGGCATCAGCACG  >  NC_000913/185331‑185585
                                                                                                                                      |                                                                                                                        
aCGCCCATGGAAATGACGGAACCTTCTTCGACAATCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCAcaccac                                                                                                                      >  1:865223/1‑139 (MQ=255)
     cATGGAAATGACGGAACCTTCTTCGACAATCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGaa                                                                                                                 >  2:1536551/1‑139 (MQ=255)
     cATGGAAATGACGGAACCTTCTTCGACAATCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGaa                                                                                                                 >  1:623404/1‑139 (MQ=255)
       tGGAAATGACGGAACCTTCTTCGACAATCACCCCTTCAACTACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCgg                                                                                                                   >  1:489818/1‑135 (MQ=255)
       tGGAAATGACGGAACCTTCTTCGACAATCACCCCTTCAACTACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCgg                                                                                                                   <  2:489818/135‑1 (MQ=255)
       tGGAAATGACGGAACCTTCTTCGACAATCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAg                                                                                                               >  2:1099052/1‑139 (MQ=255)
       tGGAAATGACGGAACCTTCTTCGACAATCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAg                                                                                                               >  1:951650/1‑139 (MQ=255)
           aaTGACGGAACCTTCTTCGACAATCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTgg                                                                                                           <  1:247082/139‑1 (MQ=255)
           aaTGACGGAACCTTCTTCGACAATCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTgg                                                                                                           <  2:164837/139‑1 (MQ=255)
           aaTGACGGAACCTTCTTCGACAATCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTgg                                                                                                           <  1:1183007/139‑1 (MQ=255)
           aaTGACGGAACCTTCTTCGACAATCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTgg                                                                                                           <  1:1536551/139‑1 (MQ=255)
                   aaCCTTCTTCGACAATCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGttttt                                                                                                   >  1:953520/1‑139 (MQ=255)
                      cttcttCGACAATCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTAcc                                                                                                <  1:1007046/139‑1 (MQ=255)
                        tcttcGACAATCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCaa                                                                                              >  2:539349/1‑139 (MQ=255)
                            cGACAATCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTg                                                                                          <  2:865223/139‑1 (MQ=255)
                                aaTCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGca                                                                                      <  1:1627017/139‑1 (MQ=255)
                                aaTCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGca                                                                                      >  2:1627017/1‑139 (MQ=255)
                                  tCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGcaca                                                                                    >  2:1043951/1‑139 (MQ=255)
                                            aaCCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACg                                                                          >  2:382049/1‑139 (MQ=255)
                                             aCCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACgg                                                                         >  1:511382/1‑139 (MQ=255)
                                                  ttCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGcc                                                                    <  1:539349/139‑1 (MQ=255)
                                                  ttCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGcc                                                                    <  2:480642/139‑1 (MQ=255)
                                                    cAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGt                                                                                                       >  2:661771/1‑102 (MQ=255)
                                                    cAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGt                                                                                                       <  1:661771/102‑1 (MQ=255)
                                                      gagCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAgg                                                                >  1:1152954/1‑139 (MQ=255)
                                                         cgcgcgcCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTAt                                                             <  2:953520/139‑1 (MQ=255)
                                                           cgcgcCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTAt                                                             >  2:1763507/1‑137 (MQ=255)
                                                           cgcgcCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTAt                                                             <  1:1763507/137‑1 (MQ=255)
                                                            gcgcCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTgg                                                                                                           <  1:1245685/90‑1 (MQ=255)
                                                            gcgcCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCaa                                                          >  1:1303715/1‑139 (MQ=255)
                                                            gcgcCGATGAAGCAATTATCGTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTgg                                                                                                           >  2:1245685/1‑90 (MQ=255)
                                                                     aaGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGc                                                 <  1:382049/139‑1 (MQ=255)
                                                                       gCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCt                                               <  1:1043951/139‑1 (MQ=255)
                                                                       gCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCt                                               <  2:1152954/139‑1 (MQ=255)
                                                                                        tgGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGcc                              <  2:623404/139‑1 (MQ=255)
                                                                                         ggTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGCCg                             >  2:7971/1‑139 (MQ=255)
                                                                                         ggTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGCCg                             <  2:951650/139‑1 (MQ=255)
                                                                                           ttGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGCCGAt                           <  1:1099052/139‑1 (MQ=255)
                                                                                             gggTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGt                                                                                                       >  2:1299548/1‑61 (MQ=255)
                                                                                             gggTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTg                                                                                          >  2:1577091/1‑74 (MQ=255)
                                                                                             gggTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTg                                                                                          <  1:1577091/74‑1 (MQ=255)
                                                                                             gggTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACgg                                                                         <  2:133405/91‑1 (MQ=255)
                                                                                             gggTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACgg                                                                         >  1:133405/1‑91 (MQ=255)
                                                                                             gggTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGccc                                                                   <  2:680836/97‑1 (MQ=255)
                                                                                             gggTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGccc                                                                   >  1:680836/1‑97 (MQ=255)
                                                                                             gggTTAGCCTGCAGCGCTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGt                                                                                                       <  1:1299548/61‑1 (MQ=255)
                                                                                              ggTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGCCGATGtt                        <  2:511382/139‑1 (MQ=255)
                                                                                                  aGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGt                                                               >  1:1025108/1‑96 (MQ=255)
                                                                                                  aGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGt                                                               <  2:1025108/96‑1 (MQ=255)
                                                                                                   gCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGTACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGCCGATGTTGACGt                   <  1:7971/139‑1 (MQ=255)
                                                                                                         aGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGCCGATGTTGACGTAAGACg             >  1:544475/1‑139 (MQ=255)
                                                                                                             gTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGt                                                                        <  2:959355/76‑1 (MQ=255)
                                                                                                             gTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGt                                                                        >  1:959355/1‑76 (MQ=255)
                                                                                                             gTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGCCGATGTTGACGTAAGACGGCAt         >  2:1091332/1‑139 (MQ=255)
                                                                                                             gTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGCCGATGTTGACGTAAGACGGCAt         >  1:145581/1‑139 (MQ=255)
                                                                                                             gTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGCCGATGTTGACGTAAGACGGCAt         <  1:1091332/139‑1 (MQ=255)
                                                                                                                  aGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAAt                                                                                             <  2:1346660/50‑1 (MQ=255)
                                                                                                                  aGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAAt                                                                                             >  1:1346660/1‑50 (MQ=255)
                                                                                                                    cACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGCCGATGTTGACGTAAGACGGCATCAGCACg  <  2:1303715/139‑1 (MQ=255)
                                                                                                                        ccgccgATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAAcc                                                        <  2:193787/81‑1 (MQ=255)
                                                                                                                        ccgccgATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAAcc                                                        >  1:193787/1‑81 (MQ=255)
                                                                                                                        ccgccgATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGcc                                                >  1:837993/1‑89 (MQ=255)
                                                                                                                        ccgccgATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGcc                                                <  2:837993/89‑1 (MQ=255)
                                                                                                                        ccgccgATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGCCGATGTTGACGTAAGACGGCATCAGc     <  1:1371185/132‑1 (MQ=255)
                                                                                                                        ccgccgATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGCCGATGTTGACGTAAGACGGCATCAGc     >  2:1371185/1‑132 (MQ=255)
                                                                                                                              aTGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTtcatc                                          <  2:235741/89‑1 (MQ=255)
                                                                                                                              aTGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTtcatc                                          >  1:235741/1‑89 (MQ=255)
                                                                                                                                      |                                                                                                                        
ACGCCCATGGAAATGACGGAACCTTCTTCGACAATCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACGCCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGCCGATGTTGACGTAAGACGGCATCAGCACG  >  NC_000913/185331‑185585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑