Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,114,502	C→T	*465* (TAG→TAA)	wcaM ←	colanic acid biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,114,502	0	C	T	100.0%	297.7 / NA	92	*465* (TAG→TAA)	wcaM	colanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/46);  total (46/46)

TTCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGC  >  NC_000913/2114369‑2114628                                                                                                                                      |                                                                                                                               ttCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTTCAGGAATGGTCGCAAATTTACTc                                                                                                                           >  1:1571711/1‑139 (MQ=255) ttCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTc                                                                                                                           >  2:642495/1‑139 (MQ=255)                   cATCAATTACCCTGAATTGATTACTGAATTACTTCTGATGTTACGCCGCTTCCTTGGGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCCTTGCCGGCAATCt                                                                                                         <  1:113968/139‑1 (MQ=255)                       aaTTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAg                                                                                                     <  2:680004/139‑1 (MQ=255)                          tACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTa                                                                                                  <  1:1606913/139‑1 (MQ=255)                               tGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAAtctt                                                                                             >  2:216965/1‑139 (MQ=255)                                      aTTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCcaca                                                                                      >  1:1148857/1‑139 (MQ=255)                                           tGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAAc                                                                                 >  1:986180/1‑139 (MQ=255)                                           tGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAAc                                                                                 >  2:61030/1‑139 (MQ=255)                                             aaTTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCgg                                                                                                               <  2:1298160/107‑1 (MQ=255)                                             aaTTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCgg                                                                                                               >  1:1298160/1‑107 (MQ=255)                                             aaTTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACaa                                                                               >  2:245250/1‑139 (MQ=255)                                               ttACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAata                                                                             <  2:986180/139‑1 (MQ=255)                                                tACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCt                                                                                                                        >  1:1757718/1‑95 (MQ=255)                                                tACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCt                                                                                                                        <  2:1757718/95‑1 (MQ=255)                                                 aCTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                           <  1:61030/139‑1 (MQ=255)                                                 aCTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                           <  1:216965/139‑1 (MQ=255)                                                 aCTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                           <  1:1709070/139‑1 (MQ=255)                                                  cTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTaa                                                                                  <  2:1691856/131‑1 (MQ=255)                                                  cTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTaa                                                                                  >  1:1691856/1‑131 (MQ=255)                                                     gtgATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                           >  1:487144/1‑135 (MQ=255)                                                     gtgATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                           <  2:487144/135‑1 (MQ=255)                                                     gtgATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGa                                                                       >  1:338580/1‑139 (MQ=255)                                                       gATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCgg                                                                                                               >  2:1038380/1‑97 (MQ=255)                                                       gATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCgg                                                                                                               <  1:310794/97‑1 (MQ=255)                                                       gATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCgg                                                                                                               <  1:1038380/97‑1 (MQ=255)                                                       gATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCgg                                                                                                               >  2:310794/1‑97 (MQ=255)                                                       gATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACAtt                                                                                    <  1:1690697/124‑1 (MQ=255)                                                       gATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACAtt                                                                                    >  2:1690697/1‑124 (MQ=255)                                                       gATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGAtt                                                                     >  1:1287341/1‑139 (MQ=255)                                                        aTGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTAc                                                                                                                             >  2:1782027/1‑82 (MQ=255)                                                        aTGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTAc                                                                                                                             <  1:1782027/82‑1 (MQ=255)                                                            tACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCt                                                                                                                        <  2:342103/83‑1 (MQ=255)                                                            tACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCt                                                                                                                        >  1:342103/1‑83 (MQ=255)                                                                   cTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGACAATCTAAAGTTAAtcttc                                                                                            >  1:1764558/1‑104 (MQ=255)                                                                   cTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGACAATCTAAAGTTAAtcttc                                                                                            <  2:1764558/104‑1 (MQ=255)                                                                        ttGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTaa                                                                                  >  1:681150/1‑109 (MQ=255)                                                                        ttGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTaa                                                                                  <  2:681150/109‑1 (MQ=255)                                                                           tgGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAAt                                                                                                           <  1:1099363/81‑1 (MQ=255)                                                                           tgGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAAt                                                                                                           >  2:1099363/1‑81 (MQ=255)                                                                                tGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                           >  2:1353850/1‑108 (MQ=255)                                                                                tGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                           <  1:1353850/108‑1 (MQ=255)                                                                                tGCAATAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCt                                                                                                                        <  2:1799567/63‑1 (MQ=255)                                                                                tGCAATAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCt                                                                                                                        >  1:1799567/1‑63 (MQ=255)                                                                                    gTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTc                                        <  1:642495/139‑1 (MQ=255)                                                                                        cATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACgg                                                 >  1:985891/1‑126 (MQ=255)                                                                                        cATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACgg                                                 <  2:985891/126‑1 (MQ=255)                                                                                            gTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTg                                                              <  1:807927/109‑1 (MQ=255)                                                                                            gTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTg                                                              >  2:807927/1‑109 (MQ=255)                                                                                            gTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACg                                                  <  1:1349307/121‑1 (MQ=255)                                                                                            gTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACg                                                  >  2:1349307/1‑121 (MQ=255)                                                                                               ccTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTttcattc                             <  1:1321909/139‑1 (MQ=255)                                                                                                  aaGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTcc                                                                                         >  2:419583/1‑76 (MQ=255)                                                                                                  aaGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTcc                                                                                         <  1:419583/76‑1 (MQ=255)                                                                                                  aaGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCAcc                          >  1:527116/1‑139 (MQ=255)                                                                                                     tATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCg                                                                                                                <  1:396253/50‑1 (MQ=255)                                                                                                     tATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCg                                                                                                                >  2:396253/1‑50 (MQ=255)                                                                                                       tGACTCCATTTTTCCAGGAATGGTCGCAAATTTAc                                                                                                                             >  2:332771/1‑35 (MQ=255)                                                                                                       tGACTCCATTTTTCCAGGAATGGTCGCAAATTTAc                                                                                                                             <  1:332771/35‑1 (MQ=255)                                                                                                       tGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCTGTTCCGGCAATCTAAAGTTAATCTTCTCcac                                                                                       >  2:1127890/1‑73 (MQ=255)                                                                                                       tGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTtctc                                                                                          <  1:465394/70‑1 (MQ=255)                                                                                                       tGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTtctc                                                                                          >  2:465394/1‑70 (MQ=255)                                                                                                       tGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCcac                                                                                       <  1:1127890/73‑1 (MQ=255)                                                                                                       tGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATg                     >  1:1613695/1‑139 (MQ=255)                                                                                                       tGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATg                     >  1:1459892/1‑139 (MQ=255)                                                                                                        gACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCgg                                                                                                               <  1:581361/48‑1 (MQ=255)                                                                                                        gACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCgg                                                                                                               >  2:581361/1‑48 (MQ=255)                                                                                                        gACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGa                    <  2:1459892/139‑1 (MQ=255)                                                                                                        gACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGa                    <  2:527116/139‑1 (MQ=255)                                                                                                             cATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGAt                                                                      <  2:863395/84‑1 (MQ=255)                                                                                                             cATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGAt                                                                      >  1:863395/1‑84 (MQ=255)                                                                                                              aTTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAAtcttc                                                                                            <  2:858393/61‑1 (MQ=255)                                                                                                              aTTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAAtcttc                                                                                            >  1:858393/1‑61 (MQ=255)                                                                                                               tttttCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTATAGTTAAt                                                                                                <  1:1200969/56‑1 (MQ=255)                                                                                                               tttttCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAAt                                                                                                >  2:1200969/1‑56 (MQ=255)                                                                                                               tttttCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTtctc                                                                                          <  2:431883/62‑1 (MQ=255)                                                                                                               tttttCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTtctc                                                                                          >  1:431883/1‑62 (MQ=255)                                                                                                               tttttCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACAt                                                                                     <  2:1476868/67‑1 (MQ=255)                                                                                                               tttttCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACAt                                                                                     >  1:1476868/1‑67 (MQ=255)                                                                                                               tttttCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTg             >  1:581158/1‑139 (MQ=255)                                                                                                                 tttCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCa           <  2:1114199/139‑1 (MQ=255)                                                                                                                         aaTGGTGGCAAATTTACTCCCTCAGTTCCGCCAATCTAAAGTTa                                                                                                  <  1:1217715/44‑1 (MQ=255)                                                                                                                         aaTGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTa                                                                                                  >  2:1217715/1‑44 (MQ=255)                                                                                                                          aTGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAAGAGAcagc  <  2:1613695/139‑1 (MQ=255)                                                                                                                             gTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACg                                                  >  1:1029616/1‑88 (MQ=255)                                                                                                                             gTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACg                                                  <  2:1029616/88‑1 (MQ=255)                                                                                                                             gTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACa                 <  1:142004/121‑1 (MQ=255)                                                                                                                             gTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACa                 >  2:142004/1‑121 (MQ=255)                                                                                                                              tCGCAAATTTACTCCCTCTGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTaa                                                                                  >  1:1019366/1‑55 (MQ=255)                                                                                                                              tCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTaa                                                                                  <  2:1019366/55‑1 (MQ=255)                                                                                                                                 cAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACg                                                  >  1:1213475/1‑84 (MQ=255)                                                                                                                                 cAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACg                                                  <  2:1213475/84‑1 (MQ=255)                                                                                                                                      |                                                                                                                               TTCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGC  >  NC_000913/2114369‑2114628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑