Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	219,364	T→C	P77P (CCA→CCG)	tsaA ←	tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	219,364	0	T	C	100.0%	150.5 / NA	42	P77P (CCA→CCG)	tsaA	tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/19);  total (23/19)

CCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCG  >  NC_000913/219230‑219496                                                                                                                                        |                                                                                                                                     ccGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGAc                                                                                                                                  >  2:97258/1‑139 (MQ=255) ccGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGAc                                                                                                                                  >  2:1519571/1‑139 (MQ=255)    aGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGca                                                                                                                               <  1:431695/139‑1 (MQ=255)     gCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGcac                                                                                                                              <  1:175203/139‑1 (MQ=255)                  gCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAg                                                                                                                 <  1:941323/139‑1 (MQ=255)                                aaaCAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCg                                                                                                    >  2:711731/1‑139 (MQ=255)                                aaaCAACCTCTTTCAGCTCTACCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGt                                                                                                   >  1:1692168/1‑139 (MQ=255)                                aaaCAACCTCTTTCAGCTCTACCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGt                                                                                                   >  2:1505918/1‑139 (MQ=255)                                aaaCAACCTCTTTCAGCTCTACCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCAAGGCGCGGCGGACGCACAGTCGGACGCCAgcc                                                                                                               <  2:960179/127‑1 (MQ=255)                                aaaCAACCTCTTTCAGCTCTACCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCAAGGCGCGGCGGACGCACAGTCGGACGCCAgcc                                                                                                               >  1:960179/1‑127 (MQ=255)                                                ctctACCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAg                                                                                                                 <  2:540317/109‑1 (MQ=255)                                                ctctACCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAg                                                                                                                 >  1:540317/1‑109 (MQ=255)                                                ctctACCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaaa                                                                                   >  1:24929/1‑139 (MQ=255)                                                ctctACCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaaa                                                                                   >  1:504471/1‑139 (MQ=255)                                                 tctACCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTtgatga                                                                                            >  2:1075694/1‑129 (MQ=255)                                                 tctACCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTtgatga                                                                                            <  1:1075694/129‑1 (MQ=255)                                                 tctACCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaaaa                                                                                  <  1:1383920/139‑1 (MQ=255)                                                   tACCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAgg                                                                                >  1:1769828/1‑139 (MQ=255)                                                    aCCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTc                                                                                                         <  2:203729/113‑1 (MQ=255)                                                    aCCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTc                                                                                                         >  1:203729/1‑113 (MQ=255)                                                    aCCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGa                                                                               >  1:402366/1‑139 (MQ=255)                                                     ccAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGAt                                                                              <  2:24929/139‑1 (MQ=255)                                                     ccAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACACACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGAt                                                                              <  2:701393/139‑1 (MQ=255)                                                      cAGCGACATGCCAATTGGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGAt                                                                              <  1:711731/139‑1 (MQ=255)                                                                ccAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGc                                                                   <  2:1692168/139‑1 (MQ=255)                                                                   aTT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGa                                                                >  2:65911/1‑139 (MQ=255)                                                                              ggCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAg                                                      <  2:504471/139‑1 (MQ=255)                                                                                            cGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGaa                                                               >  2:1307205/1‑116 (MQ=255)                                                                                            cGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGaa                                                               <  1:1307205/116‑1 (MQ=255)                                                                                               gtTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCgg                                     >  1:539752/1‑139 (MQ=255)                                                                                               gtTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCgg                                     <  2:402366/139‑1 (MQ=255)                                                                                               gtTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAAGCCGCGaa                                              >  2:125795/1‑130 (MQ=255)                                                                                               gtTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAAGCCGCGaa                                              <  1:125795/130‑1 (MQ=255)                                                                                                 tGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCGATCGTTTGATGAAAGACGAAAAGGATcc                                                                            <  2:1137708/98‑1 (MQ=255)                                                                                                 tGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATcc                                                                            >  1:1137708/1‑98 (MQ=255)                                                                                                           cccATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAgg                         <  1:97258/139‑1 (MQ=255)                                                                                                             cATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGc                                                                   <  2:495229/95‑1 (MQ=255)                                                                                                             cATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGc                                                                   >  1:495229/1‑95 (MQ=255)                                                                                                                         aCCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGc           >  1:1341880/1‑139 (MQ=255)                                                                                                                         aCCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGc           >  2:466369/1‑139 (MQ=255)                                                                                                                              cgAGGCGCGGCGGACGTACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTc      >  2:1679527/1‑139 (MQ=255)                                                                                                                                  gcgcGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCg  >  2:313364/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                     CCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATT‑GGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCG  >  NC_000913/219230‑219496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑