Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,225,044	C→T	*196* (TAG→TAA)	yohC ←	Yip1 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,225,044	0	C	T	100.0%	251.1 / NA	77	*196* (TAG→TAA)	yohC	Yip1 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/39);  total (38/39)

ACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCGG  >  NC_000913/2224911‑2225178                                                                                                                                      |                                                                                                                                        aCCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGaa                                                                                                                                    <  1:545086/139‑1 (MQ=255) aCCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGaa                                                                                                                                    <  2:200476/139‑1 (MQ=255) aCCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGaa                                                                                                                                    <  2:1403325/139‑1 (MQ=255)    gccgGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGTGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACaa                                                                                                                                 <  1:1831909/139‑1 (MQ=255)             aGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAAcc                                                                                                                        >  2:1699718/1‑139 (MQ=255)               gATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAGCGATAACCGt                                                                                                                      >  2:1802587/1‑139 (MQ=255)                 tttACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAg                                                                                                                    <  2:695218/139‑1 (MQ=255)                           cTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGAt                                                                                                                            >  1:1678696/1‑121 (MQ=255)                           cTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGAt                                                                                                                            <  2:1678696/121‑1 (MQ=255)                            tAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGtat                                                                                                        <  1:1334665/139‑1 (MQ=255)                            tAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGtat                                                                                                        <  1:106302/139‑1 (MQ=255)                                  cGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGt                                                                                                  >  1:295290/1‑139 (MQ=255)                                        catAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGc                                                                                            >  1:1329026/1‑139 (MQ=255)                                        catAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGc                                                                                            >  1:623537/1‑139 (MQ=255)                                             cGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGagca                                                                                       >  2:1253441/1‑139 (MQ=255)                                               aGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCg                                                                                                                       >  1:584195/1‑106 (MQ=255)                                               aGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCg                                                                                                                       <  2:584195/106‑1 (MQ=255)                                                 cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTa                                                                                                >  1:1096145/1‑126 (MQ=255)                                                 cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTa                                                                                                <  2:1096145/126‑1 (MQ=255)                                                 cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCAc                                                                                   >  2:833025/1‑139 (MQ=255)                                                 cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCAATGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCAc                                                                                   >  1:1295330/1‑139 (MQ=255)                                                   aaaaGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACtt                                                                                 <  2:1329026/139‑1 (MQ=255)                                                    aaaGTGCGATCGTCAGAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGATCAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGa                                                                                          >  1:1224685/1‑129 (MQ=255)                                                    aaaGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGa                                                                                          <  2:1224685/129‑1 (MQ=255)                                                          cGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCa                                                                                                              >  1:838095/1‑103 (MQ=255)                                                          cGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCa                                                                                                              <  2:838095/103‑1 (MQ=255)                                                           gATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTAcc                                                                         <  2:295290/139‑1 (MQ=255)                                                                tCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCa                                                                     >  1:427501/1‑139 (MQ=255)                                                                       agaATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGagcag                                                                                      <  2:145814/114‑1 (MQ=255)                                                                       agaATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGagcag                                                                                      >  1:145814/1‑114 (MQ=255)                                                                         aaTCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAAc                                                                                                                                   <  2:1058965/68‑1 (MQ=255)                                                                         aaTCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAAc                                                                                                                                   >  1:1058965/1‑68 (MQ=255)                                                                         aaTCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACa                                                                                                                                  >  1:336700/1‑69 (MQ=255)                                                                         aaTCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACa                                                                                                                                  <  2:336700/69‑1 (MQ=255)                                                                         aaTCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAgcagca                                                                                    >  2:1653406/1‑114 (MQ=255)                                                                         aaTCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAgcagca                                                                                    <  1:1653406/114‑1 (MQ=255)                                                                           tCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGtat                                                                                                        <  1:532486/92‑1 (MQ=255)                                                                           tCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGtat                                                                                                        >  2:532486/1‑92 (MQ=255)                                                                           tCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCa                                                         >  1:844795/1‑139 (MQ=255)                                                                           tCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCa                                                         >  1:1395967/1‑139 (MQ=255)                                                                              tGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACa                                                                                                                                  >  2:157348/1‑64 (MQ=255)                                                                              tGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACa                                                                                                                                  <  1:157348/64‑1 (MQ=255)                                                                              tGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCa                                                                                                              >  2:316133/1‑83 (MQ=255)                                                                              tGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCa                                                                                                              <  1:316133/83‑1 (MQ=255)                                                                              tGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCg                                                      <  2:844795/139‑1 (MQ=255)                                                                               gAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGt                                                     <  2:1295330/139‑1 (MQ=255)                                                                                aGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCg                                                                                                                       >  2:860210/1‑73 (MQ=255)                                                                                aGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCg                                                                                                                       <  1:860210/73‑1 (MQ=255)                                                                                   aaaTGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGaa                                                 <  1:827344/139‑1 (MQ=255)                                                                                     aTGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAg                                                                                                                    >  2:143730/1‑71 (MQ=255)                                                                                     aTGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAg                                                                                                                    <  1:143730/71‑1 (MQ=255)                                                                                          gcATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAgaga                                          >  1:993031/1‑139 (MQ=255)                                                                                           cATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTATTTAGAACAGACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGaa                                         >  1:1131553/1‑139 (MQ=255)                                                                                            aTTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCa                                                                     <  2:1596792/110‑1 (MQ=255)                                                                                            aTTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCa                                                                     >  1:1596792/1‑110 (MQ=255)                                                                                             ttGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑ccc                                                                                                                <  2:36229/66‑1 (MQ=255)                                                                                             ttGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑ccc                                                                                                                >  1:36229/1‑66 (MQ=255)                                                                                             ttGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATa                                                                                                            >  1:443385/1‑70 (MQ=255)                                                                                             ttGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATa                                                                                                            <  2:443385/70‑1 (MQ=255)                                                                                               gCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGaa                                                                                                                                    >  2:807409/1‑45 (MQ=255)                                                                                               gCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGaa                                                                                                                                    <  1:807409/45‑1 (MQ=255)                                                                                                cTGACATTTACGCCAGCAATGCAACGTTAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTaga                                            >  2:1056623/1‑131 (MQ=255)                                                                                                cTGACATTTACGCCAGCAAAGCAACGTTAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTaga                                            <  1:1056623/131‑1 (MQ=255)                                                                                                    cATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGcc                                <  2:623537/139‑1 (MQ=255)                                                                                                       ttACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTc                             <  2:1131553/139‑1 (MQ=255)                                                                                                        tACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCg                                                                                                                       >  1:830646/1‑49 (MQ=255)                                                                                                        tACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCg                                                                                                                       <  2:830646/49‑1 (MQ=255)                                                                                                        tACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTa                                     >  1:1611493/1‑130 (MQ=255)                                                                                                        tACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTa                                     <  2:1611493/130‑1 (MQ=255)                                                                                                          cGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCctc                          >  1:279686/1‑139 (MQ=255)                                                                                                          cGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCctc                          >  2:126444/1‑139 (MQ=255)                                                                                                             cAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCttt                       >  1:396949/1‑139 (MQ=255)                                                                                                               gCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTttgt                     <  2:396949/139‑1 (MQ=255)                                                                                                               gCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTttgt                     <  2:279686/139‑1 (MQ=255)                                                                                                                          tCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCa                                                                     <  1:1454643/80‑1 (MQ=255)                                                                                                                          tCAAAGAACTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCa                                                                     >  2:1454643/1‑80 (MQ=255)                                                                                                                                  cTTTTTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCgg  <  1:1699718/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        ACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAG‑CCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCGG  >  NC_000913/2224911‑2225178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑