Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,266,013	T→C	R188R (CGT→CGC)	yeiP →	elongation factor P‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,266,013	0	T	C	100.0%	207.1 / NA	58	R188R (CGT→CGC)	yeiP	elongation factor P‑like protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/30);  total (28/30)

AAAGGGGCATCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGAT  >  NC_000913/2265882‑2266141                                                                                                                                    |                                                                                                                                 aaaGGGGCATCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACt                                                                                                                           >  2:831268/1‑139 (MQ=255)              ccAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCa                                                                                                              <  1:405405/139‑1 (MQ=255)                                    gACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAgg                                                                                        <  1:99085/139‑1 (MQ=255)                                                tggtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGTTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCttttt                                                                            >  2:1214995/1‑139 (MQ=255)                                                  gtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGa                                                                                                 <  1:840499/116‑1 (MQ=255)                                                  gtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGa                                                                                                 >  2:840499/1‑116 (MQ=255)                                                  gtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                          >  2:543827/1‑139 (MQ=255)                                                  gtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                          >  1:1324127/1‑139 (MQ=255)                                                  gtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                          <  1:543827/139‑1 (MQ=255)                                                  gtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCACATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                          <  2:89588/139‑1 (MQ=255)                                                     tggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGa                                                                                                 >  2:624876/1‑113 (MQ=255)                                                     tggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGa                                                                                                 <  1:624876/113‑1 (MQ=255)                                                       gtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATtta                                                                     <  2:353373/139‑1 (MQ=255)                                                          aTTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                  >  1:451187/1‑107 (MQ=255)                                                          aTTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                  <  2:451187/107‑1 (MQ=255)                                                            tCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATtata                                                                >  2:871296/1‑139 (MQ=255)                                                            tCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATtata                                                                >  1:87203/1‑139 (MQ=255)                                                             cAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATAc                                                               <  2:1324127/139‑1 (MQ=255)                                                             cAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATAc                                                               <  1:1214995/139‑1 (MQ=255)                                                                         tACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTAtt                                                   >  2:1075453/1‑139 (MQ=255)                                                                                 cccGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGa                                                                                                 >  2:1559985/1‑85 (MQ=255)                                                                                 cccGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGa                                                                                                 <  1:1559985/85‑1 (MQ=255)                                                                                 cccGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATAc                                                               >  1:393770/1‑119 (MQ=255)                                                                                 cccGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATAc                                                               <  2:393770/119‑1 (MQ=255)                                                                                   cGGGCGAAAAAATTCGTATCCATATCGAAGAAGGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGa                                                                                                 >  1:1090580/1‑83 (MQ=255)                                                                                   cGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGa                                                                                                 <  2:1090580/83‑1 (MQ=255)                                                                                   cGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCa                                                             >  1:1343869/1‑119 (MQ=255)                                                                                   cGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCa                                                             <  2:1343869/119‑1 (MQ=255)                                                                                     ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                  <  1:1554822/80‑1 (MQ=255)                                                                                     ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                  >  2:1554822/1‑80 (MQ=255)                                                                                     ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                          >  2:1793785/1‑104 (MQ=255)                                                                                     ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                          >  2:730987/1‑104 (MQ=255)                                                                                     ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                          <  1:730987/104‑1 (MQ=255)                                                                                     ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                          <  1:1793785/104‑1 (MQ=255)                                                                                     ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTg                                       >  2:1424088/1‑139 (MQ=255)                                                                                     ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTg                                       <  1:1424088/139‑1 (MQ=255)                                                                                     ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCCTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTg                                       >  2:108189/1‑139 (MQ=255)                                                                                      gCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTaa                                                                         >  1:921142/1‑104 (MQ=255)                                                                                      gCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTaa                                                                         <  2:921142/104‑1 (MQ=255)                                                                                                  gTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGcc                          <  2:87203/139‑1 (MQ=255)                                                                                                             gaagaaCGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCCTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCa               <  1:108189/139‑1 (MQ=255)                                                                                                              aagaaCGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGaaa                                                                                                      <  2:841402/51‑1 (MQ=255)                                                                                                              aagaaCGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGaaa                                                                                                      >  1:841402/1‑51 (MQ=255)                                                                                                              aagaaCGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTc                                                                                   <  2:999265/70‑1 (MQ=255)                                                                                                              aagaaCGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTc                                                                                   >  1:999265/1‑70 (MQ=255)                                                                                                              aagaaCGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCAc              >  2:54844/1‑139 (MQ=255)                                                                                                                    gCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGAt                                                                                                >  2:1163192/1‑51 (MQ=255)                                                                                                                    gCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGAt                                                                                                <  1:1163192/51‑1 (MQ=255)                                                                                                                          atatGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTtgattgat  <  1:871296/139‑1 (MQ=255)                                                                                                                          atatGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTtgattgat  <  1:1075453/139‑1 (MQ=255)                                                                                                                                ggCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGa                                                                                                 >  2:1594293/1‑38 (MQ=255)                                                                                                                                ggCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGa                                                                                                 <  1:1594293/38‑1 (MQ=255)                                                                                                                                ggCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                          <  1:1669617/61‑1 (MQ=255)                                                                                                                                ggCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                          >  2:1669617/1‑61 (MQ=255)                                                                                                                                ggCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTgcgc                                             <  1:936420/90‑1 (MQ=255)                                                                                                                                ggCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTgcgc                                             >  2:936420/1‑90 (MQ=255)                                                                                                                                ggCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCt                        >  2:420364/1‑111 (MQ=255)                                                                                                                                ggCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCt                        <  1:420364/111‑1 (MQ=255)                                                                                                                                    |                                                                                                                                 AAAGGGGCATCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGAT  >  NC_000913/2265882‑2266141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑