Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,441,742 G→A intergenic (‑137/‑22) fabB ← / → mnmC 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I/fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,441,7420GA100.0% 246.8 / NA 76intergenic (‑137/‑22)fabB/mnmC3‑oxoacyl‑[acyl‑carrier‑protein] synthase I/fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/33);  total (43/33)

TACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAG  >  NC_000913/2441610‑2441872
                                                                                                                                    |                                                                                                                                  
tACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTa                                                                                                                              >  2:501546/1‑139 (MQ=255)
tACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTa                                                                                                                              >  1:1825366/1‑139 (MQ=255)
 aCCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTaa                                                                                                                             <  2:1044330/139‑1 (MQ=255)
   ctctGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGa                                                                                                                           >  2:1056897/1‑139 (MQ=255)
   ctctGTAAGTCCCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGa                                                                                                                           <  2:1187673/139‑1 (MQ=255)
        tAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGt                                                                                                                      <  2:99069/139‑1 (MQ=255)
          aGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTaa                                                                                                                    <  1:1652345/139‑1 (MQ=255)
          aGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTaa                                                                                                                    <  2:801727/139‑1 (MQ=255)
             cGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGAc                                                                                                                          >  1:207427/1‑130 (MQ=255)
             cGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGAc                                                                                                                          <  2:207427/130‑1 (MQ=255)
              gCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTa                                                                                                                >  2:1066000/1‑139 (MQ=255)
               cacaTAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAAc                                                                                                                   >  2:1468328/1‑135 (MQ=255)
               cacaTAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAAc                                                                                                                   <  1:1468328/135‑1 (MQ=255)
                acaTAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACg                                                                                                              >  1:1711392/1‑139 (MQ=255)
                  aTAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTg                                                                                                            <  2:362147/139‑1 (MQ=255)
                          aGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta                                                                                                    <  2:1825366/139‑1 (MQ=255)
                              tCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACg                                                                                                              >  2:21789/1‑125 (MQ=255)
                              tCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACg                                                                                                              <  1:21789/125‑1 (MQ=255)
                              tCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTcc                                                                                                >  2:432007/1‑139 (MQ=255)
                                          tAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGcc                                                                                    <  1:501546/139‑1 (MQ=255)
                                               tACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCaccacc                                                                                                                                  <  1:1216794/88‑1 (MQ=255)
                                               tACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCt                                                                               >  2:651439/1‑139 (MQ=255)
                                                    ttGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTg                                                                                                            <  2:268290/105‑1 (MQ=255)
                                                    ttGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTg                                                                                                            >  1:268290/1‑105 (MQ=255)
                                                       tACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTa                                                                       >  2:29557/1‑139 (MQ=255)
                                                       tACGCCGAACAAGTCCGATCAACCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAGCCTCGAGTTTa                                                                       <  1:1066000/139‑1 (MQ=255)
                                                            cGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  <  1:432007/139‑1 (MQ=255)
                                                            cGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  <  1:1056897/139‑1 (MQ=255)
                                                            cGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  <  1:29557/139‑1 (MQ=255)
                                                              aaCAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGa                                                                >  1:1178491/1‑139 (MQ=255)
                                                                   gTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTa                                                           >  2:1712078/1‑139 (MQ=255)
                                                                        aTCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCt                                                      >  1:1513909/1‑139 (MQ=255)
                                                                              cATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta                                                                                                    >  2:49019/1‑87 (MQ=255)
                                                                              cATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta                                                                                                    <  1:49019/87‑1 (MQ=255)
                                                                                tttAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  >  1:1518604/1‑119 (MQ=255)
                                                                                tttAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  <  2:1518604/119‑1 (MQ=255)
                                                                                  tAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCgag                                            >  1:1527671/1‑139 (MQ=255)
                                                                                       gagaAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGAtttt                                       >  1:1445999/1‑139 (MQ=255)
                                                                                          aaaTTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAaca                                                                                                       >  2:311838/1‑72 (MQ=255)
                                                                                          aaaTTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAaca                                                                                                       <  1:311838/72‑1 (MQ=255)
                                                                                          aaaTTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACactac                                                                                                   >  2:1506562/1‑76 (MQ=255)
                                                                                          aaaTTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACactac                                                                                                   <  1:1506562/76‑1 (MQ=255)
                                                                                          aaaTTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  >  1:664703/1‑109 (MQ=255)
                                                                                          aaaTTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  <  2:664703/109‑1 (MQ=255)
                                                                                          aaaTTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGAc                                    >  2:890055/1‑139 (MQ=255)
                                                                                          aaaTTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGAc                                    >  1:1435650/1‑139 (MQ=255)
                                                                                          aaaTTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGAc                                    >  1:1141661/1‑139 (MQ=255)
                                                                                             tttGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACg                                   >  2:650153/1‑137 (MQ=255)
                                                                                             tttGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACg                                   <  1:650153/137‑1 (MQ=255)
                                                                                                   cAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCTTACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTAt                           <  2:1141661/139‑1 (MQ=255)
                                                                                                           cacacaTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTc                      >  1:1742340/1‑136 (MQ=255)
                                                                                                           cacacaTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTc                      <  2:1742340/136‑1 (MQ=255)
                                                                                                                aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta                                                                                                    >  2:361858/1‑53 (MQ=255)
                                                                                                                aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta                                                                                                    <  1:361858/53‑1 (MQ=255)
                                                                                                                aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  <  1:1457252/87‑1 (MQ=255)
                                                                                                                aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  >  2:1457252/1‑87 (MQ=255)
                                                                                                                aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATa              >  2:903949/1‑139 (MQ=255)
                                                                                                                aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATa              >  1:1407617/1‑139 (MQ=255)
                                                                                                                aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATa              >  1:350729/1‑139 (MQ=255)
                                                                                                                aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATa              >  2:452069/1‑139 (MQ=255)
                                                                                                                  cGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  >  1:227864/1‑85 (MQ=255)
                                                                                                                  cGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  >  1:345010/1‑85 (MQ=255)
                                                                                                                  cGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  <  2:345010/85‑1 (MQ=255)
                                                                                                                  cGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt                                                                  <  2:227864/85‑1 (MQ=255)
                                                                                                                  cGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATTTCTATTTTTCCAACGATaa             <  2:1223433/138‑1 (MQ=255)
                                                                                                                  cGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATaa             >  1:1223433/1‑138 (MQ=255)
                                                                                                                    cTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGc                                                                                     <  2:33067/64‑1 (MQ=255)
                                                                                                                    cTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGc                                                                                     >  1:33067/1‑64 (MQ=255)
                                                                                                                          tCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATaa             >  2:346812/1‑130 (MQ=255)
                                                                                                                          tCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATaa             <  1:346812/130‑1 (MQ=255)
                                                                                                                            gAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta                                                                                                    >  1:1595055/1‑41 (MQ=255)
                                                                                                                            gAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta                                                                                                    <  2:1595055/41‑1 (MQ=255)
                                                                                                                            gAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta                                                                                                    <  1:1440278/41‑1 (MQ=255)
                                                                                                                            gAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta                                                                                                    >  2:1440278/1‑41 (MQ=255)
                                                                                                                            gAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAag  >  2:291999/1‑139 (MQ=255)
                                                                                                                            gAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAag  >  1:612399/1‑139 (MQ=255)
                                                                                                                            gAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAag  >  2:96947/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                  
TACCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAG  >  NC_000913/2441610‑2441872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑