Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,658,145 T→C Q264R (CAG→CGG)  hscA ← DnaK‑like molecular chaperone specific for IscU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,658,1450TC100.0% 224.6 / NA 63Q264R (CAG→CGG) hscADnaK‑like molecular chaperone specific for IscU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/34);  total (29/34)

TTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAA  >  NC_000913/2658010‑2658278
                                                                                                                                       |                                                                                                                                     
tttACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGaa                                                                                                                                    <  1:112972/139‑1 (MQ=255)
       gTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGtt                                                                                                                             <  2:734989/139‑1 (MQ=255)
        tgtcgggATCAGTTCATTGAATTGTTCACGGCTGATTTCGCACTGCCAGCCCACAACGTTAACGCTCACGGAATCCGCATCGCTCAGCCCGATTTTCCCTGCAATGGCGGCAACCAGCAGTTCACGCCGAACGCGGTTa                                                                                                                            >  1:1705064/7‑139 (MQ=255)
         ggCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTAt                                                                                                                           >  1:475827/1‑139 (MQ=255)
           cgcgATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCg                                                                                                                         <  1:65807/139‑1 (MQ=255)
             cgATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCt                                                                                                                       <  1:1381819/139‑1 (MQ=255)
             cgATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCt                                                                                                                       <  2:1759864/139‑1 (MQ=255)
             cgATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCt                                                                                                                       <  2:475827/139‑1 (MQ=255)
               aTCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTAc                                                                                                                     >  2:1156958/1‑139 (MQ=255)
                     tCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAg                                                                                                               <  1:1156958/139‑1 (MQ=255)
                     tCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAg                                                                                                               <  1:1455922/139‑1 (MQ=255)
                     tCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAg                                                                                                               <  2:37426/139‑1 (MQ=255)
                              tGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCg                                                                                                      >  1:1546710/1‑139 (MQ=255)
                                ttCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCt                                                                                                                       >  1:133554/1‑120 (MQ=255)
                                ttCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCt                                                                                                                       <  2:133554/120‑1 (MQ=255)
                                ttCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAg                                                                                                               >  1:761661/1‑128 (MQ=255)
                                ttCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAg                                                                                                               <  2:761661/128‑1 (MQ=255)
                                  cACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTg                                                                                                  >  1:676826/1‑139 (MQ=255)
                                  cACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTg                                                                                                  <  2:1546710/139‑1 (MQ=255)
                                    cGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTCCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCt                                                                                                <  1:409469/139‑1 (MQ=255)
                                     ggCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGt                                                                                                                              <  1:1043384/108‑1 (MQ=255)
                                     ggCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGt                                                                                                                              >  2:1043384/1‑108 (MQ=255)
                                     ggCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCt                                                                                                                       >  1:82802/1‑115 (MQ=255)
                                     ggCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCt                                                                                                                       <  2:82802/115‑1 (MQ=255)
                                          aTTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTa                                                                                                                            >  2:305468/1‑105 (MQ=255)
                                          aTTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTa                                                                                                                            <  1:305468/105‑1 (MQ=255)
                                          aTTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGaa                                                                                          >  2:142532/1‑139 (MQ=255)
                                                  cTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATcc                                                                                  <  2:676826/139‑1 (MQ=255)
                                                    gCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTg                                                                                                  <  2:1646586/121‑1 (MQ=255)
                                                    gCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTg                                                                                                  >  1:1646586/1‑121 (MQ=255)
                                                    gCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGc                                                                                >  2:195801/1‑139 (MQ=255)
                                                         cccGCAACGTTAACGGTCACGGAGTTCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCcagca                                                                           >  1:36189/1‑139 (MQ=255)
                                                                   tAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGaa                                                                 >  1:355398/1‑139 (MQ=255)
                                                                          cACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATcg                                                          >  1:33536/1‑139 (MQ=255)
                                                                           aCGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAAcg                                                                                                                                  <  1:1218430/66‑1 (MQ=255)
                                                                           aCGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAAcg                                                                                                                                  >  2:1218430/1‑66 (MQ=255)
                                                                           aCGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATcgc                                                         >  1:553505/1‑139 (MQ=255)
                                                                            cGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATcgcc                                                        >  1:981117/1‑139 (MQ=255)
                                                                               aGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCgccgcc                                                     <  1:722429/139‑1 (MQ=255)
                                                                                 tCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCt                                                                                                                       <  2:999038/71‑1 (MQ=255)
                                                                                 tCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCt                                                                                                                       >  1:999038/1‑71 (MQ=255)
                                                                                  ccGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAAt                                                                                    >  1:574744/1‑105 (MQ=255)
                                                                                  ccGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAAt                                                                                    <  2:574744/105‑1 (MQ=255)
                                                                                   cGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCt                                                                                                                       <  2:1436999/69‑1 (MQ=255)
                                                                                   cGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCt                                                                                                                       >  1:1436999/1‑69 (MQ=255)
                                                                                    gCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAgcg                                                <  2:33536/139‑1 (MQ=255)
                                                                                          cTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAAt                                                                                    <  1:587375/97‑1 (MQ=255)
                                                                                          cTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAAt                                                                                    >  2:587375/1‑97 (MQ=255)
                                                                                           tCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGc                                                                                >  2:238482/1‑100 (MQ=255)
                                                                                           tCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGc                                                                                <  1:238482/100‑1 (MQ=255)
                                                                                           tCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCgccgcc                                                     >  2:1514323/1‑127 (MQ=255)
                                                                                           tCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCgccgcc                                                     <  1:1514323/127‑1 (MQ=255)
                                                                                             aGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCAc                                       >  2:512721/1‑139 (MQ=255)
                                                                                                  gATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCAccgccg                                  <  1:195801/139‑1 (MQ=255)
                                                                                                          cTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAg                          <  1:509792/139‑1 (MQ=255)
                                                                                                              aaTGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCcagcag                                                                          <  1:1555314/87‑1 (MQ=255)
                                                                                                              aaTGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCcagcag                                                                          >  2:1555314/1‑87 (MQ=255)
                                                                                                                        tCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTTGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGcc                            <  1:1071267/123‑1 (MQ=255)
                                                                                                                        tCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGcc                            >  2:1071267/1‑123 (MQ=255)
                                                                                                                           agcagTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGGCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACAcgccgc         <  1:142532/139‑1 (MQ=255)
                                                                                                                                ttCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACtt    <  2:553505/139‑1 (MQ=255)
                                                                                                                                 tCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGa                                            >  2:427186/1‑98 (MQ=255)
                                                                                                                                 tCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGa                                            <  1:427186/98‑1 (MQ=255)
                                                                                                                                  cACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTaa  >  1:193696/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                     
TTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAA  >  NC_000913/2658010‑2658278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑