Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,713,568 T→C W225R (TGG→CGG)  srmB → ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,713,5680TC100.0% 142.3 / NA 41W225R (TGG→CGG) srmBATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/20);  total (21/20)

ACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGTGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATG  >  NC_000913/2713445‑2713682
                                                                                                                           |                                                                                                                  
aCCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCg                                                                                                     >  1:86988/1‑139 (MQ=255)
 cccTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCga                                                                                                    <  1:339142/139‑1 (MQ=255)
   cTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCgatg                                                                                                  <  2:860299/139‑1 (MQ=255)
                cAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCa                                                                                        >  1:1307369/1‑136 (MQ=255)
                cAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCa                                                                                        <  2:1307369/136‑1 (MQ=255)
                   cGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAAc                                                                                  >  2:1280427/1‑139 (MQ=255)
                     cTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCGGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACcg                                                                                <  2:1056485/139‑1 (MQ=255)
                             cgatgcgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACcg                                                                                                          <  1:524354/105‑1 (MQ=255)
                             cgatgcgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACcg                                                                                                          >  2:524354/1‑105 (MQ=255)
                              gatgcgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCg                                                                                                     <  1:71577/109‑1 (MQ=255)
                              gatgcgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCg                                                                                                     >  2:71577/1‑109 (MQ=255)
                                  cgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                   <  2:86988/139‑1 (MQ=255)
                                               tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCgatg                                                                                                  <  1:1670988/95‑1 (MQ=255)
                                               tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCgatg                                                                                                  >  2:1670988/1‑95 (MQ=255)
                                               tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCga                                                                                                    <  2:1389295/93‑1 (MQ=255)
                                               tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCga                                                                                                    >  1:1389295/1‑93 (MQ=255)
                                               tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAg                                                      >  2:1249040/1‑139 (MQ=255)
                                                                      cGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                   <  1:216318/103‑1 (MQ=255)
                                                                      cGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                   >  2:216318/1‑103 (MQ=255)
                                                                        gTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTgtgt                             <  1:1249040/139‑1 (MQ=255)
                                                                         tGGAAGTTTCTGCCAATCCCTCCACCGGGGAGCGCAGAAAAATTCAGCATCGGTATTACCGCGCCGAGGATCTTGAGAATAAAACCGCGTTGCAGGTAAATCTGTTGAAACAGCCGGAAACAAACCGCTCAATTGTGtt                            >  1:187852/1‑139 (MQ=255)
                                                                         tGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGtt                            >  2:606076/1‑139 (MQ=255)
                                                                                        aTCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAagcgtga             >  2:548607/1‑139 (MQ=255)
                                                                                         tCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACcgcg                                                                              >  1:547913/1‑73 (MQ=255)
                                                                                         tCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACcgcg                                                                              <  2:547913/73‑1 (MQ=255)
                                                                                           ccTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAagcgtgagcg          >  2:1296946/1‑139 (MQ=255)
                                                                                               cACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCtt                                                                                             >  2:1624114/1‑52 (MQ=255)
                                                                                               cACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCtt                                                                                             <  1:1624114/52‑1 (MQ=255)
                                                                                               cACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACcgcg                                                                              >  2:1291454/1‑67 (MQ=255)
                                                                                               cACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACcgcg                                                                              <  1:1291454/67‑1 (MQ=255)
                                                                                                   cGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATg  <  1:606076/139‑1 (MQ=255)
                                                                                                    gTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGc                                                     >  1:398120/1‑87 (MQ=255)
                                                                                                    gTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGc                                                     <  2:398120/87‑1 (MQ=255)
                                                                                                    gTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCg                                        >  1:1265635/1‑100 (MQ=255)
                                                                                                    gTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCg                                        <  2:1265635/100‑1 (MQ=255)
                                                                                                     tGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGt                     >  2:833393/1‑118 (MQ=255)
                                                                                                     tGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGt                     <  1:833393/118‑1 (MQ=255)
                                                                                                     tGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAagcgtgagc           <  2:479893/128‑1 (MQ=255)
                                                                                                     tGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAagcgtgagc           >  1:479893/1‑128 (MQ=255)
                                                                                                         cgcAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                   >  1:969004/1‑68 (MQ=255)
                                                                                                         cgcAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                   <  2:969004/68‑1 (MQ=255)
                                                                                                                           |                                                                                                                  
ACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGTGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATG  >  NC_000913/2713445‑2713682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑