Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,835,045	G→A	*378* (TAG→TAA)	norW →	NADH:flavorubredoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,835,045	0	G	A	100.0%	130.9 / NA	41	*378* (TAG→TAA)	norW	NADH:flavorubredoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/20);  total (21/20)

ACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTT  >  NC_000913/2834926‑2835173                                                                                                                        |                                                                                                                                 aCCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACt                                                                                                                        <  2:931186/130‑1 (MQ=255) aCCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACt                                                                                                                        >  1:931186/1‑130 (MQ=255) aCCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTaaa                                                                                                               >  2:1633105/1‑139 (MQ=255) aCCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTaaa                                                                                                               <  2:1408445/139‑1 (MQ=255)               aaTGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCT‑AAATGTCGGATGCGACg                                                                                                >  1:293874/1‑139 (MQ=255)                   gtggCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCT‑AAATGTCGGATGCGACGCTgg                                                                                            <  2:293874/139‑1 (MQ=255)                    tggCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTgg                                                                                            <  1:1415604/139‑1 (MQ=255)                         cgcgGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGt                                                                                       >  1:1749570/1‑139 (MQ=255)                                          tGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACggg                                                                      <  1:1633105/139‑1 (MQ=255)                                               aGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGc                                                                 <  2:1749570/139‑1 (MQ=255)                                                   tCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTgg                                                                                            <  2:98402/108‑1 (MQ=255)                                                   tCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTgg                                                                                            >  1:98402/1‑108 (MQ=255)                                                           ttGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTAc                                                                                                                         >  1:185274/1‑71 (MQ=255)                                                           ttGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTAc                                                                                                                         <  2:185274/71‑1 (MQ=255)                                                            tgtgGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCAtgtg                                                            >  1:310640/1‑131 (MQ=255)                                                            tgtgGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCAtgtg                                                            <  2:310640/131‑1 (MQ=255)                                                                     tGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGa                                                                    >  2:1099508/1‑114 (MQ=255)                                                                     tGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGa                                                                    <  1:1099508/114‑1 (MQ=255)                                                                     tGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGt                                           >  1:43436/1‑139 (MQ=255)                                                                     tGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGt                                           >  2:505105/1‑139 (MQ=255)                                                                          aTCGGATGAAAGA‑GCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACg                                                                                                >  1:133234/1‑80 (MQ=255)                                                                          aTCGGATGAAAGA‑GCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACg                                                                                                <  2:133234/80‑1 (MQ=255)                                                                             ggATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTAcgccg                                   <  2:43436/139‑1 (MQ=255)                                                                               aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACg                                                                                                >  2:1771138/1‑76 (MQ=255)                                                                               aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACg                                                                                                >  1:102890/1‑76 (MQ=255)                                                                               aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACg                                                                                                <  2:102890/76‑1 (MQ=255)                                                                               aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACg                                                                                                <  1:1771138/76‑1 (MQ=255)                                                                               aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACggg                                                                      <  2:1237741/102‑1 (MQ=255)                                                                               aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACggg                                                                      >  1:1237741/1‑102 (MQ=255)                                                                               aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCa                                 >  2:267776/1‑139 (MQ=255)                                                                                   aaGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTggg                                                                                                                             >  2:1554837/1‑43 (MQ=255)                                                                                   aaGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTggg                                                                                                                             <  1:1554837/43‑1 (MQ=255)                                                                                       ggCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaa                         <  2:170447/139‑1 (MQ=255)                                                                                             tGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTAcg                                      <  1:678432/120‑1 (MQ=255)                                                                                             tGGATTGTTGAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTAcg                                      >  2:678432/1‑120 (MQ=255)                                                                                                  tgttgAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCa                          <  1:999029/127‑1 (MQ=255)                                                                                                  tgttgAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCa                          >  2:999029/1‑127 (MQ=255)                                                                                                  tgttgAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCaaa              <  1:505105/139‑1 (MQ=255)                                                                                                      gAAAACATTGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCa          >  2:762770/1‑139 (MQ=255)                                                                                                              tGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGttt  >  2:734866/1‑139 (MQ=255)                                                                                                              tGCCGATGTAAGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGttt  >  1:1009442/1‑139 (MQ=255)                                                                                                                        |                                                                                                                                 ACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTT  >  NC_000913/2834926‑2835173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑