Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,015,738	G→A	*193* (TAG→TAA)	mocA →	CTP:molybdopterin cytidylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,015,738	0	G	A	100.0%	187.4 / NA	58	*193* (TAG→TAA)	mocA	CTP:molybdopterin cytidylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/31);  total (27/31)

CCAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATT  >  NC_000913/3015611‑3015873                                                                                                                                |                                                                                                                                        ccAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGtt                                                                                                                              >  1:1016628/1‑139 (MQ=255)        gCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGAt                                                                                                                       >  2:804195/1‑139 (MQ=255)                cACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCa                                                                                                               <  1:1438153/139‑1 (MQ=255)                   tCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAat                                                                                                            >  2:46173/1‑139 (MQ=255)                          gggAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAAt                                                                                                     <  1:804195/139‑1 (MQ=255)                               aTCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAAc                                                                                                                                    <  1:454790/103‑1 (MQ=255)                               aTCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAAc                                                                                                                                    >  2:454790/1‑103 (MQ=255)                               aTCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTc                                                                                                >  2:368943/1‑139 (MQ=255)                                    tatgCCGGCGAAATAGAAAATGCAGAAATAATTTTAGATATTCATACTCCTGACGACTTTATTACAGCGAAGGAAAGCTATACTGATAATTAAAAATCGTAATTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCa                                                                                           <  1:720780/139‑1 (MQ=255)                                      ttCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACt                                                                                         <  1:46173/139‑1 (MQ=255)                                      ttCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACt                                                                                         <  1:455630/139‑1 (MQ=255)                                         cGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCAt                                                                                      <  1:368943/139‑1 (MQ=255)                                           tCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCaa                                                                                                              <  1:438118/113‑1 (MQ=255)                                           tCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCaa                                                                                                              >  2:438118/1‑113 (MQ=255)                                           tCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCATTCAAATATTTAATCCTTCCgg                                                                                             >  2:1350519/1‑130 (MQ=255)                                           tCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCATTCAAATATTTAATCCTTCCgg                                                                                             <  1:1350519/130‑1 (MQ=255)                                             gAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATc                                                                                  <  1:1392259/139‑1 (MQ=255)                                                        tGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAg                                                                       <  2:261447/139‑1 (MQ=255)                                                        tGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAg                                                                       <  2:1016628/139‑1 (MQ=255)                                                           aGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGGAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGaa                                                                    >  1:1192909/1‑139 (MQ=255)                                                            gAAATAATTTTAGATATTGATACTCCGGAGGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTaaaaaa                                                                                                                                     <  2:1362622/73‑1 (MQ=255)                                                            gAAATAATTTTAGATATTGATACTCCGGAGGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTaaaaaa                                                                                                                                     >  1:1362622/1‑73 (MQ=255)                                                              aataatTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATACATCCGGCACTCATTATCATAATGATAAGGAATCa                                                                 <  1:53834/139‑1 (MQ=255)                                                                               aTACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGAtttt                                                         >  2:1351091/1‑130 (MQ=255)                                                                               aTACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGAtttt                                                         <  1:1351091/130‑1 (MQ=255)                                                                                   tCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaaaagagaaa                                            >  1:83223/1‑139 (MQ=255)                                                                                     cGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTAt                                                                                   >  2:386280/1‑98 (MQ=255)                                                                                     cGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTAt                                                                                   <  1:386280/98‑1 (MQ=255)                                                                                      ggACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATc                                                                                  <  1:610573/98‑1 (MQ=255)                                                                                      ggACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATc                                                                                  >  2:610573/1‑98 (MQ=255)                                                                                       gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAAtt                                        >  2:1475369/1‑139 (MQ=255)                                                                                             tttATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCgg                                                                                             >  1:1618078/1‑80 (MQ=255)                                                                                             tttATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCgg                                                                                             <  2:1618078/80‑1 (MQ=255)                                                                                              ttattaCAGCGCAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAACTGATAAAt                                 <  2:1192909/139‑1 (MQ=255)                                                                                               tattaCAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATg                                >  2:1429826/1‑139 (MQ=255)                                                                                                 ttaCAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATggg                              <  1:1429826/139‑1 (MQ=255)                                                                                                      gCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCaa                                                                                                              >  1:1422850/1‑54 (MQ=255)                                                                                                      gCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCaa                                                                                                              <  2:1422850/54‑1 (MQ=255)                                                                                                      gCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCa                                                                                                               <  1:1020602/53‑1 (MQ=255)                                                                                                      gCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCa                                                                                                               >  2:1020602/1‑53 (MQ=255)                                                                                                      gCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCgg                                                                                             >  2:1565284/1‑71 (MQ=255)                                                                                                      gCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCgg                                                                                             <  1:1565284/71‑1 (MQ=255)                                                                                                       cGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCa                                                                                           <  1:1087473/72‑1 (MQ=255)                                                                                                       cGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCa                                                                                           >  2:1087473/1‑72 (MQ=255)                                                                                                       cGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCAt                                                                                      <  1:1773862/77‑1 (MQ=255)                                                                                                       cGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCAt                                                                                      >  2:1773862/1‑77 (MQ=255)                                                                                                       cGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATa                                                                               <  2:1468837/84‑1 (MQ=255)                                                                                                       cGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATa                                                                               >  1:1468837/1‑84 (MQ=255)                                                                                                         aaGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACt                                                                                         >  1:793293/1‑72 (MQ=255)                                                                                                         aaGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACt                                                                                         <  2:793293/72‑1 (MQ=255)                                                                                                                    taCTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCAt                                                                                      <  2:1698097/64‑1 (MQ=255)                                                                                                                    taCTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCAt                                                                                      >  1:1698097/1‑64 (MQ=255)                                                                                                                      cTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATaa                                   >  1:1672891/1‑113 (MQ=255)                                                                                                                      cTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATaa                                   <  2:1672891/113‑1 (MQ=255)                                                                                                                           aTTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATa                                                                               <  2:1307618/64‑1 (MQ=255)                                                                                                                           aTTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATa                                                                               >  1:1307618/1‑64 (MQ=255)                                                                                                                           aTTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGa    >  1:639368/1‑139 (MQ=255)                                                                                                                            tttAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaa                                                    <  1:1211933/90‑1 (MQ=255)                                                                                                                            tttAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaa                                                    >  2:1211933/1‑90 (MQ=255)                                                                                                                             ttAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGAtt  <  1:1701057/139‑1 (MQ=255)                                                                                                                                |                                                                                                                                        CCAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATT  >  NC_000913/3015611‑3015873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑