Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,191,696	G→A	*355* (TAG→TAA)	yqiI →	fimbrial protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,191,696	0	G	A	100.0%	75.7 / NA	25	*355* (TAG→TAA)	yqiI	fimbrial protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/13);  total (12/13)

CTTTTTCATCGTCTAGTGCGCCTTCGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCAT  >  NC_000913/3191569‑3191831                                                                                                                                |                                                                                                                                        cttTTTCATCGTCTAGTGCGCCTTCGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACa                                                                                                                              <  1:1221348/139‑1 (MQ=255)                       ttCGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAgg                                                                                                        <  2:664777/139‑1 (MQ=255)                         cGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGcc                                                                                                      <  1:348037/139‑1 (MQ=255)                                    ccATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGttttagtgt                                                                                           <  1:1571217/139‑8 (MQ=255)                                               aGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGc                                                                                        <  2:1051485/131‑1 (MQ=255)                                               aGAACTGAGTGCGAACCAGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCAGCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGc                                                                                        >  1:1051485/1‑131 (MQ=255)                                                 aaCTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACa                                                                                                                              >  1:978359/1‑91 (MQ=255)                                                 aaCTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACa                                                                                                                              <  2:978359/91‑1 (MQ=255)                                                        tGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGGCGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAgg                                                                                                        >  1:1298765/1‑106 (MQ=255)                                                        tGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAgg                                                                                                        <  2:1298765/106‑1 (MQ=255)                                                                cGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAatat                                                                                                                                      >  1:501102/1‑68 (MQ=255)                                                                cGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAatat                                                                                                                                      <  2:501102/68‑1 (MQ=255)                                                                   cAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTCTATCAGTGCTGTAACTCCAGCTCTCTTGCCTGcatca                                                            >  1:1169422/1‑139 (MQ=255)                                                                           gttgttGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAAGATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGa                                                    <  1:314721/139‑1 (MQ=255)                                                                           gctgttgAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATAAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGa                                                    >  1:1100495/3‑139 (MQ=255)                                                                                               tAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTgc                                >  1:1112832/1‑139 (MQ=255)                                                                                               tAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTgc                                >  1:1103552/1‑139 (MQ=255)                                                                                                  aGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCa                             >  2:690986/1‑139 (MQ=255)                                                                                                    aCGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCAt                                                                                                            <  2:57529/58‑1 (MQ=255)                                                                                                    aCGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCAt                                                                                                            >  1:57529/1‑58 (MQ=255)                                                                                                         aTCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCt                                                                  <  1:728019/95‑1 (MQ=255)                                                                                                         aTCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCt                                                                  >  2:728019/1‑95 (MQ=255)                                                                                                         aTCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAg                      >  2:1762730/1‑139 (MQ=255)                                                                                                               aaaaTCACCTATAACTAATATCTAATACGAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTa                <  2:1100495/139‑1 (MQ=255)                                                                                                                     aCCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGttct          <  2:1112832/139‑1 (MQ=255)                                                                                                                      ccTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGttctt         >  2:1406238/1‑139 (MQ=255)                                                                                                                             cTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCAt  <  2:1169422/139‑1 (MQ=255)                                                                                                                                |                                                                                                                                        CTTTTTCATCGTCTAGTGCGCCTTCGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCAT  >  NC_000913/3191569‑3191831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑