Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,269,012	A→G	S200G (AGT→GGT)	yhaC →	pentapetide repeats‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,269,012	0	A	G	100.0%	229.2 / NA	64	S200G (AGT→GGT)	yhaC	pentapetide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/30);  total (34/30)

TCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAG  >  NC_000913/3268879‑3269141                                                                                                                                      |                                                                                                                                  tctGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCa                                                                                                                              >  1:1028232/1‑139 (MQ=255)      aCTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAAcaca                                                                                                                         <  1:183012/139‑1 (MQ=255)         tACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACaaaa                                                                                                                      >  2:487261/1‑139 (MQ=255)          aCGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCaa                                                                                                                             <  2:88257/131‑1 (MQ=255)          aCGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCaa                                                                                                                             >  1:88257/1‑131 (MQ=255)          aCGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACaa                                                                                                                        >  2:32391/1‑136 (MQ=255)          aCGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACaa                                                                                                                        <  1:32391/136‑1 (MQ=255)          aCGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAAc                                                                                                                     >  2:742026/1‑139 (MQ=255)                tGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               >  2:670635/1‑139 (MQ=255)                tGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               <  2:1028232/139‑1 (MQ=255)                tGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               <  1:742026/139‑1 (MQ=255)                 gTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACaa                                                                                                              <  2:17413/139‑1 (MQ=255)                                       tCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               >  2:241875/1‑116 (MQ=255)                                       tCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               <  1:241875/116‑1 (MQ=255)                                            gATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATaa                                                                                   >  2:879394/1‑139 (MQ=255)                                               aaaCATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGc                                                                                <  1:1751659/139‑1 (MQ=255)                                                     atGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGttt                                                                          >  2:718523/1‑139 (MQ=255)                                                       ggCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTta                                                                        >  2:1004459/1‑139 (MQ=255)                                                       ggCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTta                                                                        >  1:1182676/1‑139 (MQ=255)                                                            aTACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAaca                                                                                                                           >  2:736970/1‑83 (MQ=255)                                                            aTACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAaca                                                                                                                           >  2:1059637/1‑83 (MQ=255)                                                            aTACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAaca                                                                                                                           <  1:736970/83‑1 (MQ=255)                                                            aTACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAaca                                                                                                                           <  1:1059637/83‑1 (MQ=255)                                                            aTACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAg                                                                   >  1:1572691/1‑139 (MQ=255)                                                             tACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGt                                                                            <  1:105447/129‑1 (MQ=255)                                                             tACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGt                                                                            >  2:105447/1‑129 (MQ=255)                                                                  ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               <  1:282805/89‑1 (MQ=255)                                                                  ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               >  2:282805/1‑89 (MQ=255)                                                                   tttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTggg                                                                                                     >  2:824973/1‑98 (MQ=255)                                                                   tttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTggg                                                                                                     <  1:824973/98‑1 (MQ=255)                                                                      aaaTCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGat                                                         <  1:1004459/139‑1 (MQ=255)                                                                       aaTCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCaa                                                                                                                             <  1:1747904/70‑1 (MQ=255)                                                                       aaTCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCaa                                                                                                                             >  2:1747904/1‑70 (MQ=255)                                                                       aaTCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               <  2:439784/84‑1 (MQ=255)                                                                       aaTCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               >  1:439784/1‑84 (MQ=255)                                                                       aaTCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGatt                                                                                                  <  1:1410371/97‑1 (MQ=255)                                                                       aaTCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGatt                                                                                                  >  2:1410371/1‑97 (MQ=255)                                                                            ttttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTtaata                                                                     >  1:1565169/1‑121 (MQ=255)                                                                            ttttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTtaata                                                                     <  2:1565169/121‑1 (MQ=255)                                                                             tttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTAtt                                                  >  1:508674/1‑139 (MQ=255)                                                                                     gAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTa                                                              <  1:1575467/119‑1 (MQ=255)                                                                                     gAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTa                                                              >  2:1575467/1‑119 (MQ=255)                                                                                      aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               <  1:1118187/69‑1 (MQ=255)                                                                                      aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               >  2:1118187/1‑69 (MQ=255)                                                                                                aTTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               <  2:123481/59‑1 (MQ=255)                                                                                                aTTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               >  1:123481/1‑59 (MQ=255)                                                                                                     aCTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTg                                                                             >  1:627875/1‑88 (MQ=255)                                                                                                     aCTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTg                                                                             <  2:627875/88‑1 (MQ=255)                                                                                                         aTGCTAACATGTTTCACGTTAATTTTGTTGCTTCAACACAAAACGCCACCACCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCACTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAg                      <  1:1301498/139‑1 (MQ=255)                                                                                                           gCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACaaa                                                                                                                       <  2:1659950/40‑1 (MQ=255)                                                                                                           gCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACaaa                                                                                                                       >  1:1659950/1‑40 (MQ=255)                                                                                                           gCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGc                                                                                <  1:724281/79‑1 (MQ=255)                                                                                                           gCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGc                                                                                >  2:724281/1‑79 (MQ=255)                                                                                                           gCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGt                                                                            >  2:1165374/1‑83 (MQ=255)                                                                                                           gCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGt                                                                            <  1:1165374/83‑1 (MQ=255)                                                                                                               aCATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               <  2:898334/44‑1 (MQ=255)                                                                                                               aCATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               >  1:898334/1‑44 (MQ=255)                                                                                                                   gTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAg                                                                   <  2:394676/84‑1 (MQ=255)                                                                                                                   gTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAg                                                                   >  1:394676/1‑84 (MQ=255)                                                                                                                    tttCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               <  1:1151937/39‑1 (MQ=255)                                                                                                                    tttCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                               >  2:1151937/1‑39 (MQ=255)                                                                                                                     ttCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAAtctc                                                                                                          >  2:278424/1‑43 (MQ=255)                                                                                                                     ttCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAAtctc                                                                                                          <  1:278424/43‑1 (MQ=255)                                                                                                                             aaTTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGgag  >  2:544072/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                  TCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAG  >  NC_000913/3268879‑3269141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑