Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,361,329	G→A	G52S (GGC→AGC)	gltF →	periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,361,329	0	G	A	100.0%	234.1 / NA	72	G52S (GGC→AGC)	gltF	periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/32);  total (40/32)

AACAGCTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCGGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACATTAAATATAACCTGCACAGCACCAACGCTAATTGCTGTAAC  >  NC_000913/3361199‑3361462                                                                                                                                   |                                                                                                                                      aacaGCTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCAttgtt                                                                                                                               <  1:1398403/139‑1 (MQ=255)     gctgctATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACt                                                                                                                           <  2:606939/139‑1 (MQ=255)      ctgctATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTa                                                                                                                          >  1:1742772/1‑139 (MQ=255)      ctgctATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTa                                                                                                                          >  2:675648/1‑139 (MQ=255)        gctATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATg                                                                                                                        >  1:753333/1‑139 (MQ=255)         ctATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATgg                                                                                                                       >  1:1139035/1‑139 (MQ=255)         ctATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATgg                                                                                                                       >  2:15517/1‑139 (MQ=255)                   agcaCTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCAttgt                                                                                                                                <  2:762278/120‑1 (MQ=255)                   agcaCTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCAttgt                                                                                                                                >  1:762278/1‑120 (MQ=255)                      aCTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCG‑GTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACa                                                                                                         >  2:242133/1‑139 (MQ=255)                            tGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGggcggcagc                                                                                                                                     <  1:219749/106‑1 (MQ=255)                            tGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGggcggcagc                                                                                                                                     >  2:219749/1‑106 (MQ=255)                            tGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTgcg                                                                                                    <  1:15517/139‑1 (MQ=255)                                                 ggCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGt                                                                               >  2:1312056/1‑139 (MQ=255)                                                 ggCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGt                                                                               >  1:1557653/1‑139 (MQ=255)                                                     aCAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGt                                                                                     <  2:614840/129‑1 (MQ=255)                                                     aCAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTaa                                                                                   <  1:1615768/131‑1 (MQ=255)                                                     aCAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTaa                                                                                   >  2:1615768/1‑131 (MQ=255)                                                     aCAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCAGACCGGt                                                                                     >  1:614840/1‑129 (MQ=255)                                                       aGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATaaa                                                                         <  1:300155/139‑1 (MQ=255)                                                       aGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATaaa                                                                         <  1:675648/139‑1 (MQ=255)                                                       aGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGCTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATaaa                                                                         <  1:1312056/139‑1 (MQ=255)                                                        gATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAAc                                                                        >  1:594619/1‑139 (MQ=255)                                                                 gATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTgcg                                                                                                    <  1:326740/102‑1 (MQ=255)                                                                 gATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTgcg                                                                                                    >  2:326740/1‑102 (MQ=255)                                                                    aCAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGaaa                                                                                                                   >  2:1623021/1‑84 (MQ=255)                                                                    aCAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGaaa                                                                                                                   <  1:1623021/84‑1 (MQ=255)                                                                                  catcGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGa                                                                                                                             >  2:1437726/1‑60 (MQ=255)                                                                                  catcGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGa                                                                                                                             <  1:1437726/60‑1 (MQ=255)                                                                                  catcGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGAc                                                                                                                            <  2:1718156/61‑1 (MQ=255)                                                                                  catcGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGAc                                                                                                                            >  1:1718156/1‑61 (MQ=255)                                                                                  catcGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTgcg                                                                                                    >  1:491073/1‑85 (MQ=255)                                                                                  catcGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTgcg                                                                                                    <  2:491073/85‑1 (MQ=255)                                                                                      ggCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACAt                                          <  2:753333/139‑1 (MQ=255)                                                                                      ggCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACAt                                          <  2:875601/139‑1 (MQ=255)                                                                                       gCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACAtt                                         >  1:432888/1‑139 (MQ=255)                                                                                           gTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACAt                                          >  1:1446271/1‑134 (MQ=255)                                                                                           gTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACAt                                          <  2:1446271/134‑1 (MQ=255)                                                                                            tataCCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACATTAAata                                    >  1:1111247/1‑139 (MQ=255)                                                                                                  ccTGGTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACATTAAATATAACCt                              >  2:1339861/1‑139 (MQ=255)                                                                                                     ggTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGa                                                                                                                     >  1:12447/1‑49 (MQ=255)                                                                                                     ggTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGa                                                                                                                     <  2:12447/49‑1 (MQ=255)                                                                                                     ggTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCaaaaa                                                  <  2:1339486/116‑1 (MQ=255)                                                                                                     ggTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCaaaaa                                                  >  1:1339486/1‑116 (MQ=255)                                                                                                     ggTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACATTAAATATAACCTGca                           >  2:22736/1‑139 (MQ=255)                                                                                                      gTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGa                                                                                                                             <  2:542852/40‑1 (MQ=39)                                                                                                      gTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGa                                                                                                                             >  1:542852/1‑40 (MQ=255)                                                                                                      gTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACt                                                                                                                           >  2:1488313/1‑42 (MQ=255)                                                                                                      gTGCATGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACt                                                                                                                           <  1:1488313/42‑1 (MQ=255)                                                                                                           tGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGa                                                                                                                     >  2:1541508/1‑43 (MQ=255)                                                                                                           tGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGa                                                                                                                     <  1:1541508/43‑1 (MQ=255)                                                                                                           tGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTaa                                                                                   <  2:142769/77‑1 (MQ=255)                                                                                                           tGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTaa                                                                                   >  1:142769/1‑77 (MQ=255)                                                                                                           tGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATaaa                                                                         <  1:303305/87‑1 (MQ=255)                                                                                                           tGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATaaa                                                                         >  2:303305/1‑87 (MQ=255)                                                                                                           tGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTgg                                                            >  2:675485/1‑100 (MQ=255)                                                                                                           tGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTgg                                                            <  1:675485/100‑1 (MQ=255)                                                                                                           tGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGcaca                                           <  2:681434/117‑1 (MQ=255)                                                                                                           tGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGcaca                                           >  1:681434/1‑117 (MQ=255)                                                                                                           tGTACCCCAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACATTAAATATAACCTGCACAGCAc                     >  2:1236253/1‑139 (MQ=255)                                                                                                                 ccAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGaaa                                                                                                                   <  2:67079/39‑1 (MQ=255)                                                                                                                 ccAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGaaa                                                                                                                   >  1:67079/1‑39 (MQ=38)                                                                                                                  cAGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACATTAAATATAACCTGCACAGCACCAACGCt              <  1:22736/139‑1 (MQ=255)                                                                                                                   aGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTg                                                                                                      >  1:1495632/1‑50 (MQ=255)                                                                                                                   aGTTGTTACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTg                                                                                                      <  2:1495632/50‑1 (MQ=255)                                                                                                                    gttgttACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAAATGGGCCGCAAAAACAGCACATTAAATATAACCTGCACAGCACCAACGCTaa            >  1:1016446/1‑139 (MQ=255)                                                                                                                      tgttACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACATTAAATATAACCTGCACAGCACCAACGCTAAtt          >  2:404213/1‑139 (MQ=255)                                                                                                                        ttACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACATTAAATATAACCTGCACAGCACCAACGCTAATTGc        >  2:817108/1‑139 (MQ=255)                                                                                                                         tACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACAt                                          >  2:575410/1‑104 (MQ=255)                                                                                                                         tACGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACAt                                          <  1:575410/104‑1 (MQ=255)                                                                                                                          aCGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACATTAAATATAACCTGCACAGCACCa                   >  2:370254/1‑126 (MQ=255)                                                                                                                          aCGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACATTAAATATAACCTGCACAGCACCa                   <  1:370254/126‑1 (MQ=255)                                                                                                                          aCGGGCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACATTAAATATAACCTGCACAGCACCAACGCTAATTGCTg      >  1:588566/1‑139 (MQ=255)                                                                                                                            gggCGGCAGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACATTAAATATAACCTGCACAGCACCAACGCTAATTGCTGTa    <  2:1557653/139‑1 (MQ=255)                                                                                                                              gcggcagcATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAAc                                                                        >  1:1433624/1‑69 (MQ=255)                                                                                                                              gcggcagcATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAAc                                                                        <  2:1433624/69‑1 (MQ=255)                                                                                                                              gcggcagcATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAGAACAGCACATTAAATATAACCTGCACAGCACCAACGCTAATTGCTGTAAc  >  2:1161883/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                      AACAGCTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTATACCCCTGGTGCATGTACCCCAGTTGTTACGGGCGGCGGCATTGTTGACTATGGGAAACATCATAACAGTGCGCTGAATCCGACCGGTAAAAGTAATAAACTGGTTCAACTGGGCCGCAAAAACAGCACATTAAATATAACCTGCACAGCACCAACGCTAATTGCTGTAAC  >  NC_000913/3361199‑3361462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑