Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,398,875 C→T M1M (GTG→ATG) †
*368* (TAG→TAA
mreD ←
mreC ←
cell wall structural complex MreBCD transmembrane component MreD
cell wall structural complex MreBCD transmembrane component MreC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,398,8750CT100.0% 148.8 / NA 46M1M (GTG→ATG) †
*368* (TAG→TAA
mreD
mreC
cell wall structural complex MreBCD transmembrane component MreD
cell wall structural complex MreBCD transmembrane component MreC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/21);  total (25/21)

AATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTG  >  NC_000913/3398740‑3399006
                                                                                                                                       |                                                                                                                                   
aaTACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTAt                                                                                                                                  >  2:383363/1‑139 (MQ=255)
aaTACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTAt                                                                                                                                  <  1:1672827/139‑1 (MQ=255)
       caaGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTc                                                                                                                           >  1:1092900/1‑139 (MQ=255)
               gTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCa                                                                                                                   <  2:1092900/139‑1 (MQ=255)
                   cacCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGcc                                                                                                                              <  1:857275/124‑1 (MQ=255)
                   cacCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGcc                                                                                                                              >  2:857275/1‑124 (MQ=255)
                          tttGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCgg                                                                                                        >  1:352292/1‑139 (MQ=255)
                             ggCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTg                                                                                                     >  2:838064/1‑139 (MQ=255)
                                                 aTCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGcc                                                                                                                              <  2:1693590/94‑1 (MQ=255)
                                                 aTCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGcc                                                                                                                              >  1:1693590/1‑94 (MQ=255)
                                                   ccGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTa                                                                               <  2:352292/139‑1 (MQ=255)
                                                      gCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcggcggcg                                                                                 <  1:1528038/134‑1 (MQ=255)
                                                      gCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcggcggcg                                                                                 >  2:1528038/1‑134 (MQ=255)
                                                      gCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGcc                                                                            >  1:1535057/1‑139 (MQ=255)
                                                        cAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCa                                                                                                                   <  1:624108/98‑1 (MQ=255)
                                                        cAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCa                                                                                                                   >  2:624108/1‑98 (MQ=255)
                                                                  tttGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGagag                                                                <  1:383363/139‑1 (MQ=255)
                                                                  tttGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGagag                                                                <  2:598546/139‑1 (MQ=255)
                                                                               cgcAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTg                                                                                                                                <  1:1102682/62‑1 (MQ=255)
                                                                               cgcAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTg                                                                                                                                >  2:1102682/1‑62 (MQ=255)
                                                                                         aaaGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTcc                                                                                                                          >  1:421788/1‑58 (MQ=255)
                                                                                         aaaGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTcc                                                                                                                          <  2:421788/58‑1 (MQ=255)
                                                                                                gCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcggcg                                                                                    >  2:1069755/1‑89 (MQ=255)
                                                                                                gCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcggcg                                                                                    <  1:1069755/89‑1 (MQ=255)
                                                                                                gCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTa                                                                               >  1:1027855/1‑94 (MQ=255)
                                                                                                gCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTa                                                                               <  2:1027855/94‑1 (MQ=255)
                                                                                                gCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCgcagca                                  >  1:1471043/1‑139 (MQ=255)
                                                                                                  cAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTagc                                                        <  1:936870/115‑1 (MQ=255)
                                                                                                  cAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTagc                                                        >  2:936870/1‑115 (MQ=255)
                                                                                                        aCCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGt                          <  1:838064/139‑1 (MQ=255)
                                                                                                              cGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGga                                                                   >  2:691646/1‑92 (MQ=255)
                                                                                                              cGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTGGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGga                                                                   <  1:691646/92‑1 (MQ=255)
                                                                                                               gTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCgg                                                                                                        >  1:699419/1‑54 (MQ=255)
                                                                                                               gTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCgg                                                                                                        <  2:699419/54‑1 (MQ=255)
                                                                                                                 cccTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCAtt                 >  1:833997/1‑139 (MQ=255)
                                                                                                                           gATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGc                                                                             >  1:899139/1‑69 (MQ=255)
                                                                                                                           gATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGc                                                                             <  2:899139/69‑1 (MQ=255)
                                                                                                                           gATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGcc       >  2:91617/1‑139 (MQ=255)
                                                                                                                            aTAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAg                           >  2:992873/1‑118 (MQ=255)
                                                                                                                            aTAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAg                           <  1:992873/118‑1 (MQ=255)
                                                                                                                                cTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCgg                                                                                                        >  1:194167/1‑37 (MQ=255)
                                                                                                                                cTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCgg                                                                                                        <  2:194167/37‑1 (MQ=255)
                                                                                                                                cTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGca                                                                                                 >  1:3196/1‑44 (MQ=255)
                                                                                                                                cTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGca                                                                                                 <  2:3196/44‑1 (MQ=255)
                                                                                                                                cTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTtg  >  1:686842/1‑139 (MQ=255)
                                                                                                                                cTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTtg  >  1:1128946/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                   
AATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTG  >  NC_000913/3398740‑3399006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: