Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,652,144	G→A	intergenic (+113/+38)	yhiS → / ← insH1	pseudogene/IS5 transposase and trans‑activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,652,144	0	G	A	100.0%	14.5 / NA	7	intergenic (+113/+38)	yhiS/insH1	pseudogene/IS5 transposase and trans‑activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/2);  total (5/2)

TATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTT  >  NC_000913/3652010‑3652162                                                                                                                                       |                   tataAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAAACCg                >  1:1430638/1‑139 (MQ=255) tataAATTTCACTGACGCAAATACAGGGAAGGTGCGAGTAAACGGGGAAATTCTTCTCGGCTGTCTCCGTCCATTCCGGTCTTCTTGCTCGCGCCGATTTTTTCTCCCGTAAATGCCTTGAATAAATCTATTTAAACCg                >  1:722795/1‑139 (MQ=9)      aTTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAAACCGTttct           >  1:707590/1‑139 (MQ=255)      aTTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAAACCGTttct           >  2:227974/1‑139 (MQ=255)          cACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAAACCGTTTCTTCGc       <  2:769503/139‑1 (MQ=255)           aCTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAAACCGTTTCTTCGcc      >  2:566482/1‑139 (MQ=38)               aCGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAAACCGTTTCTTCGCCAttt  >  2:788033/1‑139 (MQ=21)               aCGCAAAGTTAGGGAAGGTCCGAATAAGCGGGGACATTCTTCTCGGCTGACTCAGTTATTTCATTTCTTAATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAAACCTTTTCTTCGCCAttt  <  1:62696/139‑1 (MQ=11)                                                                                                                                       |                   TATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTT  >  NC_000913/3652010‑3652162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑