Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,862,497 C→T pseudogene (1107/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,862,4970CT100.0% 313.6 / NA 96pseudogene (1107/1617 nt)glvCpseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/50);  total (46/50)

GCACATGTTGAACTGCAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCG  >  NC_000913/3862362‑3862627
                                                                                                                                        |                                                                                                                                  
gCACATGTTGAACTGCAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAg                                                                                                                                 >  1:911572/1‑139 (MQ=255)
 cacaTGTTGAACTGCAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGt                                                                                                                                >  2:111223/1‑139 (MQ=255)
      gTTGAACTGCAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATTCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAAt                                                                                                                            >  1:1221289/1‑139 (MQ=255)
      gTTGAACTGCAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATc                                                                                                                           >  2:429544/1‑139 (MQ=255)
       ttGAACTGCAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCa                                                                                                                          <  2:1333814/139‑1 (MQ=255)
          aaCTGCAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGAc                                                                                                                       >  2:756205/1‑139 (MQ=255)
            cTGCAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCt                                                                                                                     >  2:160153/1‑139 (MQ=255)
               cAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCg                                                                                                                  <  2:732601/139‑1 (MQ=255)
                 gAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGcc                                                                                                                <  2:404938/139‑1 (MQ=255)
                      aGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGtt                                                                                                          <  1:1513212/139‑1 (MQ=255)
                        tgtgCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTca                                                                                                        <  1:756205‑M1/139‑3 (MQ=255)
                         gtgCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcac                                                                                                       >  2:161525‑M1/1‑136 (MQ=255)
                         gtgCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcac                                                                                                       <  1:161525‑M1/139‑4 (MQ=255)
                           gCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcacca                                                                                                     >  1:917347‑M1/1‑134 (MQ=255)
                            cGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccac                                                                                                    <  1:429544‑M1/139‑7 (MQ=255)
                                aaaCCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacacca                                                                                                <  1:160153‑M1/139‑11 (MQ=255)
                                aaaCCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacacca                                                                                                <  2:1520396‑M1/139‑11 (MQ=255)
                                  aCCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaa                                                                                              <  1:1262174‑M1/139‑13 (MQ=255)
                                  aCCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaa                                                                                              <  2:911572‑M1/139‑13 (MQ=255)
                                  aCCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcacc                                                                                                      >  1:1701472‑M1/1‑127 (MQ=255)
                                  aCCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcacc                                                                                                      <  2:1701472‑M1/131‑5 (MQ=255)
                                   ccACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaag                                                                                             <  2:917347‑M1/139‑14 (MQ=255)
                                                   ggTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCg                                                                                                                  <  2:279463/103‑1 (MQ=255)
                                                   ggTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCg                                                                                                                  >  1:279463/1‑103 (MQ=255)
                                                   ggTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcg                                                                             >  1:440252‑M1/1‑110 (MQ=255)
                                                     tAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCTCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggt                                                                               >  1:1604794‑M1/1‑108 (MQ=255)
                                                     tAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCTCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggt                                                                               <  2:1604794‑M1/135‑28 (MQ=255)
                                                     tAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGccatg                                                                                                             >  2:880519/1‑103 (MQ=255)
                                                     tAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGccatg                                                                                                             <  1:880519/106‑4 (MQ=255)
                                                     tAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGtt                                                                                                          <  1:23357/108‑1 (MQ=255)
                                                     tAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGtt                                                                                                          >  2:23357/1‑108 (MQ=255)
                                                     tAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacgttacggtcgac                                                                           >  2:1192963‑M1/1‑108 (MQ=255)
                                                     tAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgac                                                                           >  2:220867‑M1/1‑108 (MQ=255)
                                                        aGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaa                                                                                              >  2:425877‑M1/1‑105 (MQ=255)
                                                        aGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaa                                                                                              <  1:425877‑M1/117‑13 (MQ=255)
                                                           acaACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgag                                                                     <  1:629671‑M1/139‑38 (MQ=255)
                                                             aaCCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGtt                                                                                                          >  2:1345719/1‑100 (MQ=255)
                                                             aaCCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGtt                                                                                                          <  1:1345719/100‑1 (MQ=255)
                                                             aaCCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaag                                                                                             >  2:853601‑M1/1‑100 (MQ=255)
                                                             aaCCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaag                                                                                             <  1:853601‑M1/113‑14 (MQ=255)
                                                             aaCCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacac                                                                                                  <  1:947502‑M1/108‑9 (MQ=255)
                                                             aaCCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGGCAATCAGACCTCCGCCATGTTcaccacac                                                                                                  >  2:947502‑M1/1‑100 (MQ=255)
                                                              aCCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGcc                                                                                                                >  2:711212/1‑94 (MQ=255)
                                                              aCCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGcc                                                                                                                <  1:711212/94‑1 (MQ=255)
                                                               cccAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGcc                                                                                                                <  1:850644/93‑1 (MQ=255)
                                                               cccAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGcc                                                                                                                >  2:850644/1‑93 (MQ=255)
                                                               cccAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccg                                                                 >  2:1511298‑M1/1‑98 (MQ=255)
                                                                ccAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgc                                                                <  1:1511298‑M1/139‑43 (MQ=255)
                                                                    tGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGccat                                                                                                              <  2:905166/90‑3 (MQ=255)
                                                                    tGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGccat                                                                                                              >  1:905166/1‑88 (MQ=255)
                                                                    tGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagc                                                            >  1:616944‑M1/1‑93 (MQ=255)
                                                                     ggCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGCATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagca                                                           <  1:1192963‑M1/139‑48 (MQ=255)
                                                                       cGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacg                                                         <  1:111223‑M1/139‑50 (MQ=255)
                                                                         aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggc                                                       >  2:635434‑M1/1‑88 (MQ=255)
                                                                         aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggc                                                       >  1:1801084‑M1/1‑88 (MQ=255)
                                                                         aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcga                                                                            <  2:1536862‑M1/118‑31 (MQ=255)
                                                                         aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcga                                                                            >  1:1536862‑M1/1‑88 (MQ=255)
                                                                               gTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccg                                                                 >  1:766286‑M1/1‑82 (MQ=255)
                                                                               gTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccg                                                                 <  2:766286‑M1/123‑42 (MQ=255)
                                                                                tCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAg                                                                                                                                 <  2:195511/59‑1 (MQ=255)
                                                                                tCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAg                                                                                                                                 >  1:195511/1‑59 (MQ=255)
                                                                                    catcatATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaa                                            >  2:1431083‑M1/1‑77 (MQ=255)
                                                                                          atCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagca                                      <  2:440252‑M1/139‑69 (MQ=255)
                                                                                            tCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaa                                     <  2:1221289‑M1/139‑70 (MQ=255)
                                                                                              cgcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacg                                   <  1:220867‑M1/139‑72 (MQ=255)
                                                                                               gcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagc                                                            <  2:378596‑M1/113‑47 (MQ=255)
                                                                                               gcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagc                                                            >  1:378596‑M1/1‑67 (MQ=255)
                                                                                                cgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTCAAACTTAGTCAATCAGACCTCCGccat                                                                                                              <  1:1092183/63‑3 (MQ=255)
                                                                                                cgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGccac                                                                                                              >  2:1092183/1‑61 (MQ=255)
                                                                                                cgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggt                                 <  2:616944‑M1/139‑74 (MQ=255)
                                                                                                   ggTTGCTGAACATCGGGATCCAGTTTTGCGGTAAATCTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaa                              <  2:1153886‑M1/139‑77 (MQ=255)
                                                                                                    gTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaa                              <  1:1073660‑M1/138‑77 (MQ=255)
                                                                                                    gTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaa                              >  2:1073660‑M1/1‑62 (MQ=255)
                                                                                                     ttGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacag                                        <  2:794408‑M1/127‑67 (MQ=255)
                                                                                                     ttGCTGAACATCGGGATCCAGTTTTGAGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacag                                        >  1:794408‑M1/1‑61 (MQ=255)
                                                                                                               tCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagca                                      >  2:1809488‑M1/1‑51 (MQ=255)
                                                                                                               tCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagca                                      <  1:1809488‑M1/119‑69 (MQ=255)
                                                                                                               tCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacag                                        >  2:1714827‑M1/1‑51 (MQ=255)
                                                                                                               tCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacag                                        <  1:1714827‑M1/117‑67 (MQ=255)
                                                                                                                  ggATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGcc                                                                                                                <  2:1749572/43‑1 (MQ=255)
                                                                                                                  ggATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGcc                                                                                                                >  1:1749572/1‑43 (MQ=255)
                                                                                                                  ggATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgcctgcacggc                                                       <  1:1202565‑M1/99‑52 (MQ=255)
                                                                                                                  ggATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggc                                                       >  2:1202565‑M1/1‑48 (MQ=255)
                                                                                                                     tCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTT‑‑‑CACCACACCaaa                                                                                           <  1:1428382/57‑1 (MQ=255)
                                                                                                                     tCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTT‑‑‑CACCACACCaaa                                                                                           >  2:1428382/1‑57 (MQ=255)
                                                                                                                          ttttGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaaggtaaattccag       >  1:283317‑M1/1‑40 (MQ=255)
                                                                                                                          ttttGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaaggtaaattccag       <  1:1431083‑M1/139‑100 (MQ=255)
                                                                                                                          ttttGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacg                                   <  1:757313‑M1/111‑72 (MQ=255)
                                                                                                                          ttttGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacg                                   >  2:757313‑M1/1‑40 (MQ=255)
                                                                                                                               cGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggc                                                                   <  1:332943‑M1/74‑40 (MQ=255)
                                                                                                                               cGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggc                                                                   >  2:332943‑M1/1‑35 (MQ=255)
                                                                                                                               cGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagagacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaaggtaaattccagcggtt  >  1:1132126‑M1/1‑35 (MQ=255)
                                                                                                                                 gTAAAACTTAGTCAATCAGACCTCCGCCATGTT‑‑‑CACCACACCAAAGAGATACa                                                                                   >  1:885782/1‑53 (MQ=255)
                                                                                                                                 gTAAAACTTAGTCAATCAGACCTCCGCCATGTT‑‑‑CACCACACCAAAGAGATACa                                                                                   <  2:885782/53‑1 (MQ=255)
                                                                                                                                    aaaCTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggc                                                       <  1:411355‑M1/81‑52 (MQ=255)
                                                                                                                                    aaaCTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggc                                                       >  2:411355‑M1/1‑30 (MQ=255)
                                                                                                                                        |                                                                                                                                  
GCACATGTTGAACTGCAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCG  >  NC_000913/3862362‑3862627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: