Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,870,441 G→A *355* (TAG→TAA) 
G430G (GGC→GGT) 		 cbrA →
dgoT ←		 colicin M resistance protein, FAD‑binding protein, putative oxidoreductase
D‑galactonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,870,4410GA100.0% 238.6 / NA 75*355* (TAG→TAA) 
G430G (GGC→GGT) 		cbrA
dgoT		colicin M resistance protein, FAD‑binding protein, putative oxidoreductase
D‑galactonate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/36);  total (39/36)

CTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATG  >  NC_000913/3870305‑3870576
                                                                                                                                        |                                                                                                                                       
cTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAAcc                                                                                                                                       >  1:23529/1‑139 (MQ=255)
    cTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAAcg                                                                                                                                   <  2:1154547/139‑1 (MQ=255)
        cgcgcCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCt                                                                                                                               >  1:1775335/1‑139 (MQ=255)
          cgcCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTc                                                                                                                             >  2:853823/1‑139 (MQ=255)
          cgcCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTc                                                                                                                             >  1:995375/1‑139 (MQ=255)
            ccACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTcac                                                                                                                           >  1:222177/1‑139 (MQ=255)
                  gCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGcc                                                                                                                     <  2:1319130/139‑1 (MQ=255)
                        tGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                               <  2:1775335/139‑1 (MQ=255)
                        tGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                               <  2:1699464/139‑1 (MQ=255)
                          cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             >  1:348120/1‑139 (MQ=255)
                          cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             <  1:207517/139‑1 (MQ=255)
                          cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             <  2:348120/139‑1 (MQ=255)
                          cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             <  2:23529/139‑1 (MQ=255)
                          cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             <  2:222177/139‑1 (MQ=255)
                                                          ccGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                               <  1:561156/105‑1 (MQ=255)
                                                          ccGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                               >  2:561156/1‑105 (MQ=255)
                                                          ccGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcg                                                                             >  1:1722576/1‑139 (MQ=255)
                                                              tGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAATGATTAACCAGCGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCTCGCCAATCAACGCGAcggcgg                                                                         <  1:13680/139‑1 (MQ=255)
                                                              tGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                         <  1:853823/139‑1 (MQ=255)
                                                                        cACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAgg                                                                                                           >  1:917943/1‑95 (MQ=255)
                                                                        cACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAgg                                                                                                           <  2:917943/95‑1 (MQ=255)
                                                                        cACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAAc                                                               >  1:2737/1‑139 (MQ=255)
                                                                                ttAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGCTGAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGa                                                                               >  1:479415/1‑115 (MQ=255)
                                                                                ttAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGa                                                                               <  2:479415/115‑1 (MQ=255)
                                                                                  aaGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGt                                                     >  2:413023/1‑139 (MQ=255)
                                                                                   aGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcgg                                                                            >  1:1657831/1‑115 (MQ=255)
                                                                                   aGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcgg                                                                            <  2:1657831/115‑1 (MQ=255)
                                                                                      aaGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                                 <  2:1722576/139‑1 (MQ=255)
                                                                                       aGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGccc                                                                                                                    >  1:197578/1‑71 (MQ=255)
                                                                                       aGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGccc                                                                                                                    <  2:197578/71‑1 (MQ=255)
                                                                                       aGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             >  2:217346/1‑78 (MQ=255)
                                                                                       aGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             <  1:217346/78‑1 (MQ=255)
                                                                                            aTTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGt                                           >  1:83813/1‑139 (MQ=255)
                                                                                                tGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                         <  2:158696/105‑1 (MQ=255)
                                                                                                tGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                         >  1:158696/1‑105 (MQ=255)
                                                                                                      gtggtgTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                         <  2:1043003/99‑1 (MQ=255)
                                                                                                      gtggtgTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                         >  1:1043003/1‑99 (MQ=255)
                                                                                                       tggtgTGGCACATATTCCACAGTTGAAGGGTTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATACGAGAGCGCGCCAATCAACGCGAcggcgg                                                                         <  2:1138241/98‑1 (MQ=255)
                                                                                                       tggtgTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                         >  1:1138241/1‑98 (MQ=255)
                                                                                                       tggtgTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGcc                                >  1:1835249/1‑139 (MQ=255)
                                                                                                          tgtgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                               <  1:324357/57‑1 (MQ=255)
                                                                                                          tgtgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                               >  2:324357/1‑57 (MQ=255)
                                                                                                            tgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAgg                                                                                                           >  1:373087/1‑59 (MQ=255)
                                                                                                            tgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAgg                                                                                                           <  2:373087/59‑1 (MQ=255)
                                                                                                            tgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                         <  1:1638498/93‑1 (MQ=255)
                                                                                                            tgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                         >  2:1638498/1‑93 (MQ=255)
                                                                                                                 caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACAt                                                                                                                         >  2:131041/1‑40 (MQ=255)
                                                                                                                 caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACAt                                                                                                                         <  1:131041/40‑1 (MQ=255)
                                                                                                                   tatTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                         >  2:760551/1‑86 (MQ=255)
                                                                                                                   tatTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                         <  1:760551/86‑1 (MQ=255)
                                                                                                                   tatTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGc                                                         <  1:1812382/102‑1 (MQ=255)
                                                                                                                   tatTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGc                                                         >  2:1812382/1‑102 (MQ=255)
                                                                                                                   tatTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCccacc                    >  2:1529689/1‑139 (MQ=255)
                                                                                                                    atTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGccc                                                                                                                    >  2:886831/1‑42 (MQ=255)
                                                                                                                    atTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGccc                                                                                                                    <  1:886831/42‑1 (MQ=255)
                                                                                                                        cacaGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAgg                                                                                                           <  2:1726133/47‑1 (MQ=255)
                                                                                                                        cacaGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAgg                                                                                                           >  1:1726133/1‑47 (MQ=255)
                                                                                                                         acaGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGata                                                                    >  1:126455/1‑85 (MQ=255)
                                                                                                                         acaGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGata                                                                    <  2:126455/85‑1 (MQ=255)
                                                                                                                            gTTGAAGGATTAACCAACGCGCTTCACATCGCccacc                                                                                                                 >  2:994369/1‑37 (MQ=255)
                                                                                                                            gTTGAAGGATTAACCAACGCGCTTCACATCGCccacc                                                                                                                 <  1:994369/37‑1 (MQ=255)
                                                                                                                            gTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                               >  2:140974/1‑39 (MQ=255)
                                                                                                                            gTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                               <  1:140974/39‑1 (MQ=255)
                                                                                                                             ttGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                               >  1:935/1‑38 (MQ=255)
                                                                                                                             ttGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                               <  2:935/38‑1 (MQ=255)
                                                                                                                             ttGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGaa          >  1:651198/1‑139 (MQ=255)
                                                                                                                             ttGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGaa          >  1:508645/1‑139 (MQ=255)
                                                                                                                                aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcag                                                                         <  2:344767/73‑3 (MQ=255)
                                                                                                                                aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcag                                                                         >  1:344767/1‑71 (MQ=255)
                                                                                                                                aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCg                                                  <  2:1259462/96‑1 (MQ=255)
                                                                                                                                aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCg                                                  >  1:1259462/1‑96 (MQ=255)
                                                                                                                                aaGGATTAACCAACGCGCTTCACATCGCCCACCAGAAGGATGTAAGAGAGGGCGCCAATCAACGCGACGGCGGAGATATAAACGAGTGCAGGTGCGAAACTGTAACCCTGCGCCAGGTTCCCCACGACCagcggagcgg       >  1:576698/1‑139 (MQ=255)
                                                                                                                                  ggATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCa                                                                                         <  2:352158/55‑1 (MQ=255)
                                                                                                                                  ggATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCa                                                                                         >  1:352158/1‑55 (MQ=255)
                                                                                                                                     ttAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATg  <  1:413023/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
CTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATG  >  NC_000913/3870305‑3870576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑