Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,075,454	G→A	*262* (TAG→TAA)	yihW →	putative transcriptional regulator for sulphoquinovose utilization

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,075,454	0	G	A	100.0%	218.2 / NA	67	*262* (TAG→TAA)	yihW	putative transcriptional regulator for sulphoquinovose utilization
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/37);  total (30/37)

ATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATT  >  NC_000913/4075318‑4075585                                                                                                                                         |                                                                                                                                    aTGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAAt                                                                                                                                   >  1:1752700/1‑139 (MQ=255)  tGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAAtt                                                                                                                                  >  2:543321/1‑139 (MQ=255)     ttCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAAttttt                                                                                                                               >  1:236859/1‑139 (MQ=255)     ttCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAAttttt                                                                                                                               >  1:1781299/1‑139 (MQ=255)      tCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTtg                                                                                                                              <  2:1752700/139‑1 (MQ=255)       cggcggcggTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTtgt                                                                                                                             >  1:841551/1‑139 (MQ=255)                  gAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTATCGACGAGCTGCCGCCCGCTGCGGTAAAATCACGCTTACAAGACATCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                                  <  1:1125349/139‑1 (MQ=255)                  gAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                                  <  2:1172054/139‑1 (MQ=255)                  gAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                                  <  1:1413533/139‑1 (MQ=255)                  gAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                                  <  2:213242/139‑1 (MQ=255)                  gAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                                  <  2:265558/139‑1 (MQ=255)                         gTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTtgt                                                                                                                             >  2:149632/1‑121 (MQ=255)                         gTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTtgt                                                                                                                             <  1:149632/121‑1 (MQ=255)                                      ggTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCcaca                                                                                              <  2:108290/139‑1 (MQ=255)                                      ggTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCcaca                                                                                              <  2:841551/139‑1 (MQ=255)                                        tCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATa                                                                                            >  1:1456557/1‑139 (MQ=255)                                          aCTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGc                                                                                          <  1:1708506/139‑1 (MQ=255)                                                ctctTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAAcc                                                                                    <  1:1234536/139‑1 (MQ=255)                                                     tACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                                  >  2:629113/1‑104 (MQ=255)                                                     tACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                                  <  1:629113/104‑1 (MQ=255)                                                           cgaGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTccc                                                                         >  1:751869/1‑139 (MQ=255)                                                             aGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                                  <  1:1729892/96‑1 (MQ=255)                                                             aGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                                  >  2:1729892/1‑96 (MQ=255)                                                             aGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCt                                                                                                                 <  1:1596253/97‑1 (MQ=255)                                                             aGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCt                                                                                                                 >  2:1596253/1‑97 (MQ=255)                                                             aGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGc                                                                       >  2:732423/1‑139 (MQ=255)                                                             aGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGc                                                                       >  2:281756/1‑139 (MQ=255)                                                             aGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGc                                                                       >  2:929837/1‑139 (MQ=255)                                                             aGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGc                                                                       >  1:1383104/1‑139 (MQ=255)                                                               cTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTa                                                                     <  2:1781299/139‑1 (MQ=255)                                                               cTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTa                                                                     <  1:929837/139‑1 (MQ=255)                                                                   cgcccgcTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGt                                                                 <  2:991748/139‑1 (MQ=255)                                                                       cgcTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                                  <  1:1721862/86‑1 (MQ=255)                                                                       cgcTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                                  >  2:1721862/1‑86 (MQ=255)                                                                              cTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTtgt                                                      <  2:236859/139‑1 (MQ=255)                                                                               tAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTtgtg                                                     >  2:1536000/1‑139 (MQ=255)                                                                                       cGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGaaa                                             <  1:1522358/139‑1 (MQ=255)                                                                                         cTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAagg                                           >  2:1369269/1‑139 (MQ=255)                                                                                            aCAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCca                                                                                                <  1:1791394/83‑1 (MQ=255)                                                                                            aCAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCca                                                                                                >  2:1791394/1‑83 (MQ=255)                                                                                                  cAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCaaaaa                                  <  2:751869/139‑1 (MQ=255)                                                                                                    gCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATg                                <  2:1383104/139‑1 (MQ=255)                                                                                                      cAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCaa                                     <  1:665300/132‑1 (MQ=255)                                                                                                      cAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCaa                                     >  2:665300/1‑132 (MQ=255)                                                                                                      cAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGct                              <  1:543321/139‑1 (MQ=255)                                                                                                        aaTTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAaggagg                                        <  1:725852/127‑1 (MQ=255)                                                                                                        aaTTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAaggagg                                        >  2:725852/1‑127 (MQ=255)                                                                                                        aaTTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGctct                            >  2:1357173/1‑139 (MQ=255)                                                                                                          ttGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCtat                          <  1:1357173/139‑1 (MQ=255)                                                                                                          ttGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCtat                          <  2:592777/139‑1 (MQ=255)                                                                                                          ttGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCtat                          <  1:1369269/139‑1 (MQ=255)                                                                                                                   ttcttcCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGAt                 <  2:559526/139‑1 (MQ=255)                                                                                                                        ccccAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTg                                                                                                   <  2:795713/52‑1 (MQ=255)                                                                                                                        ccccAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTg                                                                                                   >  1:795713/1‑52 (MQ=255)                                                                                                                              gAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGt                                                                 >  2:878796/1‑80 (MQ=255)                                                                                                                              gAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGt                                                                 <  1:878796/80‑1 (MQ=255)                                                                                                                              gAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAAtgt      >  2:756682/1‑139 (MQ=255)                                                                                                                                 gACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTAc                                                                    <  1:1379099/74‑1 (MQ=255)                                                                                                                                 gACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTAc                                                                    >  2:1379099/1‑74 (MQ=255)                                                                                                                                 gACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGAt   <  1:732423/139‑1 (MQ=255)                                                                                                                                  aCGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAaggagg                                        <  2:491288/101‑1 (MQ=255)                                                                                                                                  aCGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAaggagg                                        >  1:491288/1‑101 (MQ=255)                                                                                                                                  aCGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGctct                            <  2:1134645/113‑1 (MQ=255)                                                                                                                                  aCGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGctct                            >  1:1134645/1‑113 (MQ=255)                                                                                                                                  aCGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGAtt  >  1:1447032/1‑139 (MQ=255)                                                                                                                                    gcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGc                                                                                          >  2:494580/1‑49 (MQ=255)                                                                                                                                    gcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGc                                                                                          <  1:494580/49‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    ATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATT  >  NC_000913/4075318‑4075585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑