Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	372,966	A→G	T16A (ACC→GCC)	mhpF →	acetaldehyde‑CoA dehydrogenase II, NAD‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	372,966	0	A	G	100.0%	234.8 / NA	65	T16A (ACC→GCC)	mhpF	acetaldehyde‑CoA dehydrogenase II, NAD‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/31);  total (34/31)

TGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTACCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGAT  >  NC_000913/372830‑373099                                                                                                                                         |                                                                                                                                      tGAATGCGGGCGATCGTTTTGAAGGCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGcc                                                                                                                                     >  2:1121550/1‑139 (MQ=255)     tGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATc                                                                                                                                 >  2:515502/1‑139 (MQ=255)     tGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATc                                                                                                                                 >  2:1807798/1‑139 (MQ=255)         ggCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCtgat                                                                                                                             <  1:1121550/139‑1 (MQ=255)          gCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCtgatg                                                                                                                            >  2:1730640/1‑139 (MQ=255)                         cccATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGt                                                                                                             <  1:1807798/139‑1 (MQ=255)                          ccATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTc                                                                                                            <  1:1730640/139‑1 (MQ=255)                             tatTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACg                                                                                                         <  1:515502/139‑1 (MQ=255)                                tGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTc                                                                                                      <  1:323774/139‑1 (MQ=255)                                    ggCATAGGTTCGGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCa                                                                                                  >  1:538763/1‑139 (MQ=255)                                    ggCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCa                                                                                                  >  2:486373/1‑139 (MQ=255)                                       aTAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCt                                                                                               >  2:1195682/1‑139 (MQ=255)                                        tAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTg                                                                                              >  1:1525843/1‑139 (MQ=255)                                         aGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTc                                                                                                      >  1:933900/1‑130 (MQ=255)                                         aGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTc                                                                                                      <  2:933900/130‑1 (MQ=255)                                         aGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTgg                                                                                             >  1:174318/1‑139 (MQ=255)                                             tcggTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGAt                                                                                         <  2:538763/136‑1 (MQ=255)                                              cAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATg                                                                                        <  2:1525843/139‑1 (MQ=255)                                                          cATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATgg                                                                                       >  2:783291/1‑128 (MQ=255)                                                          cATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATgg                                                                                       <  1:783291/128‑1 (MQ=255)                                                          cATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGtt                                                                            <  2:455869/139‑1 (MQ=255)                                                              ttCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTc                                                                                                      >  1:200597/1‑109 (MQ=255)                                                              ttCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTc                                                                                                      <  2:200597/109‑1 (MQ=255)                                                                    cgcAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATc                                                                  >  2:137467/1‑139 (MQ=255)                                                                    cgcAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATc                                                                  >  2:124710/1‑139 (MQ=255)                                                                        gCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCa                                                              >  2:1039445/1‑139 (MQ=255)                                                                        gCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCa                                                              <  1:1039445/139‑1 (MQ=255)                                                                         ccccAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCtg                                                                                                                               >  2:363489/1‑73 (MQ=255)                                                                         ccccAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCtg                                                                                                                               <  1:363489/73‑1 (MQ=255)                                                                         ccccAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGga                                                                                            <  1:1076328/108‑1 (MQ=255)                                                                         ccccAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGga                                                                                            >  2:1076328/1‑108 (MQ=255)                                                                         ccccAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAg                                                             >  1:1645083/1‑139 (MQ=255)                                                                         ccccAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAg                                                             >  1:1472511/1‑139 (MQ=255)                                                                            cAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTcc                                                          >  2:524171/1‑139 (MQ=255)                                                                             aaaaGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCg                                                         <  1:246220/139‑1 (MQ=255)                                                                                   aaGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCa                                                                                                           >  1:697858/1‑83 (MQ=255)                                                                                   aaGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCa                                                                                                           <  2:697858/83‑1 (MQ=255)                                                                                   aaGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTc                                                   >  1:794966/1‑139 (MQ=255)                                                                                      tCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTg                                                                                  >  1:223098/1‑105 (MQ=255)                                                                                      tCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTg                                                                                  <  2:223098/105‑1 (MQ=255)                                                                                        tGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATc                                                                  <  1:1485348/119‑1 (MQ=255)                                                                                        tGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATc                                                                  >  2:1485348/1‑119 (MQ=255)                                                                                          tCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGgcgcg                                            <  2:174318/139‑1 (MQ=255)                                                                                                    gCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGAt                                                                                         <  2:1465969/84‑1 (MQ=255)                                                                                                    gCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGAt                                                                                         >  1:1465969/1‑84 (MQ=255)                                                                                                         aaGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATggg                             >  2:1564577/1‑139 (MQ=255)                                                                                                                 aTTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGcca                     <  1:124710/139‑1 (MQ=255)                                                                                                                  ttATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGccac                    <  1:1133409/139‑1 (MQ=255)                                                                                                                        gTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGgcg                                              >  2:1566746/1‑107 (MQ=255)                                                                                                                        gTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGgcg                                              <  1:1566746/107‑1 (MQ=255)                                                                                                                          tCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATg            <  1:1195682/139‑1 (MQ=255)                                                                                                                            tGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGAt                                                                                         <  1:45679/60‑1 (MQ=255)                                                                                                                            tGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGAt                                                                                         >  2:45679/1‑60 (MQ=255)                                                                                                                             gtcAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCCCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAg         >  2:733202/3‑139 (MQ=255)                                                                                                                             ggCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAg         >  1:150711/1‑139 (MQ=255)                                                                                                                              gCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCa                                                                                                           >  2:1683395/1‑40 (MQ=255)                                                                                                                              gCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCa                                                                                                           <  1:1683395/40‑1 (MQ=255)                                                                                                                              gCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGcca                     <  1:376937/126‑1 (MQ=255)                                                                                                                              gCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGcca                     >  2:376937/1‑126 (MQ=255)                                                                                                                              gCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAgg        <  1:486373/139‑1 (MQ=255)                                                                                                                                 aCATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTgg                                                                          <  2:684287/70‑1 (MQ=255)                                                                                                                                 aCATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTgg                                                                          >  1:684287/1‑70 (MQ=255)                                                                                                                                   aTTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGtt                                                                            <  2:1509470/66‑1 (MQ=255)                                                                                                                                   aTTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGtt                                                                            >  1:1509470/1‑66 (MQ=255)                                                                                                                                    ttGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGAt  <  2:794966/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      TGAATGCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTACCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGAT  >  NC_000913/372830‑373099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑