Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,279,849 A→G intergenic (+21/‑131) ghxP → / → yjcE guanine/hypoxanthine permease, high affinity, guanine/hypoxanthine:H+ symporter/putative cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,279,8490AG100.0% 235.1 / NA 66intergenic (+21/‑131)ghxP/yjcEguanine/hypoxanthine permease, high affinity, guanine/hypoxanthine:H+ symporter/putative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/35);  total (31/35)

TCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAAC  >  NC_000913/4279713‑4279977
                                                                                                                                        |                                                                                                                                
tCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCg                                                                                                                                >  1:1134544/1‑139 (MQ=255)
       tctGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCa                                                                                                                         <  1:586555/139‑1 (MQ=255)
       tctGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCa                                                                                                                         >  1:1733369/1‑139 (MQ=255)
               ttGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGc                                                                                                                 <  2:406597/139‑1 (MQ=255)
                tGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGcc                                                                                                                <  1:1828738/139‑1 (MQ=255)
                   tcgtcTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCgg                                                                                                                               >  1:72906/1‑121 (MQ=255)
                   tcgtcTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCgg                                                                                                                               <  2:72906/121‑1 (MQ=255)
                   tcgtcTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGt                                                                                                             >  2:246235/1‑139 (MQ=255)
                     gtcTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGcc                                                                                                                <  2:1809581/134‑1 (MQ=255)
                     gtcTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGcc                                                                                                                >  1:1809581/1‑134 (MQ=255)
                         ggTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTAt                                                                                                       <  2:1342933/139‑1 (MQ=255)
                               cGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTtctc                                                                                                 <  2:498900/139‑1 (MQ=255)
                                 gCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCg                                                                                               <  1:1685850/139‑1 (MQ=255)
                                    aaTGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcac                                                                                            >  1:1475505/1‑139 (MQ=255)
                                            aaGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGTTGCCCGTTTTTATTTTCTCCGCACATtgtgtg                                                                                    <  2:1134544/139‑1 (MQ=255)
                                                 gAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCtt                                                                               <  2:418460/139‑1 (MQ=255)
                                                       cGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGa                                                                         >  1:1146737/1‑139 (MQ=255)
                                                         gTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGcc                                                                                                                <  1:546028/98‑1 (MQ=255)
                                                         gTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGcc                                                                                                                >  2:546028/1‑98 (MQ=255)
                                                         gTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGata                                                                       >  2:1492009/1‑139 (MQ=255)
                                                               tCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCtt                                                                               >  1:1434398/1‑125 (MQ=255)
                                                               tCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCtt                                                                               <  2:1434398/125‑1 (MQ=255)
                                                                 gTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca                                                                                           <  1:168690/111‑1 (MQ=255)
                                                                 gTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca                                                                                           >  2:168690/1‑111 (MQ=255)
                                                                         cGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTATCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATgag                                                       <  1:1711860/139‑1 (MQ=255)
                                                                             gcgcTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAAtt                                                   <  2:1475505/139‑1 (MQ=255)
                                                                                       ccTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCg                                                                          <  1:933454/106‑1 (MQ=255)
                                                                                       ccTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCg                                                                          >  2:933454/1‑106 (MQ=255)
                                                                                        cTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCg                                                                                               >  1:1450681/1‑84 (MQ=255)
                                                                                        cTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCg                                                                                               <  2:1450681/84‑1 (MQ=255)
                                                                                        cTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACg                                        >  2:767526/1‑139 (MQ=255)
                                                                                             aTGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCttt                                   >  2:237313/1‑139 (MQ=255)
                                                                                                  ggTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCgg                                                                                                                               >  1:1342235/1‑42 (MQ=255)
                                                                                                  ggTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCgg                                                                                                                               <  2:1342235/42‑1 (MQ=255)
                                                                                                  ggTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAg                              >  2:961055/1‑139 (MQ=255)
                                                                                                  ggTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAg                              >  2:1469661/1‑139 (MQ=255)
                                                                                                  ggTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAg                              >  1:779919/1‑139 (MQ=255)
                                                                                                   gTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGa                             <  2:779919/139‑1 (MQ=255)
                                                                                                   gTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGa                             <  1:237313/139‑1 (MQ=255)
                                                                                                     ggCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTtct                                 <  1:1479869/133‑1 (MQ=255)
                                                                                                     ggCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTtct                                 >  2:1479869/1‑133 (MQ=255)
                                                                                                      gCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTg                                                                                                                  >  2:1417528/1‑51 (MQ=255)
                                                                                                      gCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTg                                                                                                                  <  1:1417528/51‑1 (MQ=255)
                                                                                                      gCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCa                          >  2:1646388/1‑139 (MQ=255)
                                                                                                               tCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCa                               <  2:1716608/125‑1 (MQ=255)
                                                                                                               tCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCa                               >  1:1716608/1‑125 (MQ=255)
                                                                                                                  aaTCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCTGATCATAAAAAACATCg              <  2:1146737/139‑1 (MQ=255)
                                                                                                                  aaTCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCg              <  1:1469661/139‑1 (MQ=255)
                                                                                                                     cTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCaa           <  1:961055/139‑1 (MQ=255)
                                                                                                                      tttCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCtt                                                                               <  1:172129/70‑1 (MQ=255)
                                                                                                                      tttCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCtt                                                                               >  2:172129/1‑70 (MQ=255)
                                                                                                                         cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTAt                                                                                                       >  1:1660818/1‑43 (MQ=255)
                                                                                                                         cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTAt                                                                                                       <  2:1660818/43‑1 (MQ=255)
                                                                                                                         cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca                                                                                           >  1:1151804/1‑55 (MQ=255)
                                                                                                                         cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca                                                                                           <  2:1151804/55‑1 (MQ=255)
                                                                                                                         cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGca                                                                                             >  1:1484357/1‑53 (MQ=255)
                                                                                                                         cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGca                                                                                             <  2:1484357/53‑1 (MQ=255)
                                                                                                                         cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATa                                           <  2:1569014/103‑1 (MQ=255)
                                                                                                                         cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATa                                           >  1:1569014/1‑103 (MQ=255)
                                                                                                                         cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAg       >  1:448732/1‑139 (MQ=255)
                                                                                                                           tCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGa                             >  2:1267536/1‑115 (MQ=255)
                                                                                                                           tCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGa                             <  1:1267536/115‑1 (MQ=255)
                                                                                                                            cGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGa    <  1:1492009/139‑1 (MQ=255)
                                                                                                                              gATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAAc  <  2:448732/139‑1 (MQ=255)
                                                                                                                                   tGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATtgtgt                                                                                     >  1:1329591/1‑51 (MQ=255)
                                                                                                                                   tGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATtgtgt                                                                                     <  2:1329591/51‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                
TCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAAC  >  NC_000913/4279713‑4279977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: